################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BanI BbeI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoII ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 837 GAATTC [GA]^AATT[CT] AflIII 863 ACACGT A^C[GA][CT]GT AlwI 795 GATCC GGATC(4/5) ApoI 837 GAATTC [GA]^AATT[CT] Asp700I 148 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 160 GTGCTC G[AT]GC[AT]^C AviII 629 TGCGCA TGC^GCA BanII 327 GGGCTC G[GA]GC[CT]^C BbsI 358 GTCTTC GAAGAC(2/6) BbvCI 291 GCTGAGG CCTCAGC(-5/-2) BglI 610 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 358 GTCTTC GAAGAC(2/6) BsgI 307 GTGCAG GTGCAG(16/14) BsiHKAI 160 GTGCTC G[AT]GC[AT]^C BspMI 881 ACCTGC ACCTGC(4/8) BsrBI 648 CCGCTC CCGCTC(-3/-3) BsrI 240 ACTGG ACTGG(1/-1) BssSI 462 CTCGTG CACGAG(-5/-1) DdeI 292 CTGAG C^T[ACGT]AG EcoRI 837 GAATTC G^AATTC FspI 629 TGCGCA TGC^GCA HaeII 386 AGCGCC [GA]GCGC^[CT] MspA1I 904 CCGCTG C[AC]G^C[GT]G NheI 684 GCTAGC G^CTAGC PaeR7I 526 CTCGAG C^TCGAG PstI 907 CTGCAG CTGCA^G SfcI 907 CTGCAG C^T[GA][CT]AG SfiI 609 GGCCATCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 530 AGGCCT AGG^CCT TaqI 527 TCGA T^CGA TthHB8I 527 TCGA T^CGA XhoI 526 CTCGAG C^TCGAG XmnI 148 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 300 GACGCC G[GA]^CG[CT]C 718 GGCGTC BbrPI 391 CACGTG CAC^GTG 864 CACGTG BfaI 685 CTAG C^TAG 715 CTAG BmyI 160 GTGCTC G[AGT]GC[ACT]^C 327 GGGCTC BsaHI 300 GACGCC G[GA]^CG[CT]C 718 GGCGTC BsiEI 901 CGGCCG CG[GA][CT]^CG 919 CGGCCG Bsp1286I 160 GTGCTC G[AGT]GC[ACT]^C 327 GGGCTC BstAPI 564 GCATTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 632 GCATCTACTGC BstEII 582 GGTGACC G^GT[ACGT]ACC 702 GGTCACC BstPI 582 GGTGACC G^GT[ACGT]ACC 702 GGTCACC DpnII 235 GATC ^GATC 795 GATC EagI 901 CGGCCG C^GGCCG 919 CGGCCG EclXI 901 CGGCCG C^GGCCG 919 CGGCCG Eco52I 901 CGGCCG C^GGCCG 919 CGGCCG EcoO65I 582 GGTGACC G^GT[ACGT]ACC 702 GGTCACC Hin1I 300 GACGCC G[GA]^CG[CT]C 718 GGCGTC MaeI 685 CTAG C^TAG 715 CTAG MboI 235 GATC ^GATC 795 GATC MboII 359 TCTTC GAAGA(8/7) 590 TCTTC NdeII 235 GATC ^GATC 795 GATC PmaCI 391 CACGTG CAC^GTG 864 CACGTG PmlI 391 CACGTG CAC^GTG 864 CACGTG PpuMI 878 GGGACCT [GA]G^G[AT]CC[CT] 955 GGGACCC Sau3AI 235 GATC ^GATC 795 GATC SfaNI 632 GCATC GCATC(5/9) 752 GCATC SmaI 320 CCCGGG CCC^GGG 960 CCCGGG SphI 440 GCATGC GCATG^C 1010 GCATGC Tsp509I 55 AATT ^AATT 838 AATT XcmI 62 CCAAGGAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 596 CCATCATCCTGTTGG XmaCI 320 CCCGGG C^CCGGG 960 CCCGGG XmaI 320 CCCGGG C^CCGGG 960 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AvaI AvaII BalI BbvI BcnI BpmI BsaAI BsaJI BsiYI BslI BsmAI BsmBI BsmFI BsoBI BsrDI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MluNI MnlI MscI MslI MspI MvaI MwoI NciI NlaIII NlaIV NspI RsaI Sau96I ScrFI SmlI StyI TseI Tsp45I TspRI