(Circular) (Six-base) MAPSORT of: pFN21AB7153.seq Check: 1252 from: 1 to: 5764 pFN21AB7153 5764 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2350 2737 3827 5211 5479 1632 Size: 718 387 1090 1384 268 1917 Fragments arranged by size: 1917 1384 1090 718 387 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 2868 4663 5640 1203 Size: 1665 1795 977 1327 Fragments arranged by size: 1795 1665 1327 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 2714 1166 Size: 1548 4216 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2337 2468 3038 1184 Size: 1153 131 570 3910 Fragments arranged by size: 3910 1153 570 131 AlwNI CAG_nnn'CTG Cuts at: 3507 5079 3507 Size: 1572 4192 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 2842 3073 3299 3451 5560 1493 Size: 1349 231 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 231 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 2325 2531 2965 3089 1870 Size: 73 382 206 434 124 4545 Fragments arranged by size: 4545 434 382 206 124 73 BamHI G'GATC_C Cuts at: 3094 3461 3094 Size: 367 5397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3085 4540 618 Size: 325 217 627 1298 1455 1842 Fragments arranged by size: 1842 1455 1298 627 325 217 BanII G_rGCy'C Cuts at: 1391 2336 3083 1391 Size: 945 747 4072 Fragments arranged by size: 4072 945 747 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2356 928 Size: 971 457 4336 Fragments arranged by size: 4336 971 457 BbvCI CC'TCA_GC Cuts at: 2297 2297 Size: 5764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3955 3989 1903 Size: 34 2018 34 3678 Fragments arranged by size: 3678 2018 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2621 4375 136 Size: 107 122 71 631 605 949 1754 1525 Fragments arranged by size: 1754 1525 949 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 3825 4981 1790 Size: 2035 1156 2573 Fragments arranged by size: 2573 2035 1156 BmgBI CAC'GTC Cuts at: 1318 3030 1318 Size: 1712 4052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3495 4453 442 Size: 416 53 83 399 24 2078 958 1753 Fragments arranged by size: 2078 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2693 1056 Size: 1637 4127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2113 2557 2113 Size: 444 5320 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2980 3026 3065 4424 1239 Size: 1741 46 39 1359 2579 Fragments arranged by size: 2579 1741 1359 46 39 Bpu10I CC'TnA_GC Cuts at: 2297 2297 Size: 5764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2641 2684 3889 4754 5052 2641 Size: 43 1205 865 298 3353 Fragments arranged by size: 3353 1205 865 298 43 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2398 2632 2871 3555 493 Size: 711 1194 234 239 684 2702 Fragments arranged by size: 2702 1194 711 684 239 234 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2306 2724 3571 3953 Size: 53 83 186 936 773 418 847 382 Cuts at: 3953 4588 275 Size: 635 1451 Fragments arranged by size: 1451 936 847 773 635 418 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4197 4595 4869 5016 5427 4197 Size: 398 274 147 411 4534 Fragments arranged by size: 4534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5539 Fragments arranged by size: 5539 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5764 BseYI C'CCAG_C Cuts at: 4967 5463 4967 Size: 496 5268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 1163 Size: 573 597 4594 Fragments arranged by size: 4594 597 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2909 2927 3214 3975 5003 1540 Size: 275 186 908 18 287 761 1028 2301 Fragments arranged by size: 2301 1028 908 761 287 275 186 18 BsiHKAI G_wGCw'C Cuts at: 2169 3825 3910 4981 2169 Size: 1656 85 1071 2952 Fragments arranged by size: 2952 1656 1071 85 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 BsmBI CGTCTCn'nnnn_ Cuts at: 2066 2084 2836 2066 Size: 18 752 4994 Fragments arranged by size: 4994 752 18 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2336 3083 3825 3910 4981 1391 Size: 399 379 167 747 742 85 1071 2174 Fragments arranged by size: 2174 1071 747 742 399 379 167 85 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2895 3119 844 Size: 2051 224 3489 Fragments arranged by size: 3489 2051 224 BsrBI CCG'CTC Cuts at: 1394 1823 2655 3651 1394 Size: 429 832 996 3507 Fragments arranged by size: 3507 996 832 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2169 2211 2403 4259 4433 65 Size: 1183 921 42 192 1856 174 1396 Fragments arranged by size: 1856 1396 1183 921 192 174 42 BsrFI r'CCGG_y Cuts at: 1700 1810 4408 4595 1700 Size: 110 2598 187 2869 Fragments arranged by size: 2869 2598 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 2467 3824 4836 2467 Size: 1357 1012 3395 Fragments arranged by size: 3395 1357 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2575 2643 794 Size: 1781 68 3915 Fragments arranged by size: 3915 1781 68 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 2587 2707 1184 Size: 1403 120 4241 Fragments arranged by size: 4241 1403 120 BstXI CCAn_nnnn'nTGG Cuts at: 2149 3004 3043 2149 Size: 855 39 4870 Fragments arranged by size: 4870 855 39 BstYI r'GATC_y Cuts at: 1972 3094 3461 3846 3863 5315 5401 5413 5759 Size: 1122 367 385 17 1452 86 12 346 Cuts at: 5759 1972 Size: 1977 Fragments arranged by size: 1977 1452 1122 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2365 487 Size: 683 56 1139 3886 Fragments arranged by size: 3886 1139 683 56 BtsI GCAGTG_nn' Cuts at: 2321 2921 3280 4074 4094 2321 Size: 600 359 794 20 3991 Fragments arranged by size: 3991 794 600 359 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2376 2400 Size: 54 1425 50 275 18 399 147 24 Cuts at: 2400 2613 2673 2906 2924 3211 4100 5729 8 Size: 213 60 233 18 287 889 1629 43 Fragments arranged by size: 1629 1425 889 399 287 275 233 213 147 60 54 50 43 24 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2906 2924 3211 1537 Size: 275 1094 18 287 4090 Fragments arranged by size: 4090 1094 287 275 18 EarI CTCTTCn'nnn_ Cuts at: 1353 3694 1353 Size: 2341 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2350 2737 2980 3026 3065 3827 4424 Size: 393 718 387 243 46 39 762 597 Cuts at: 4424 5211 5479 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 762 718 597 393 387 268 243 46 39 EcoO109I rG'GnC_Cy Cuts at: 2463 2884 2961 2463 Size: 421 77 5266 Fragments arranged by size: 5266 421 77 EcoRI G'AATT_C Cuts at: 2842 3073 3451 2842 Size: 231 378 5155 Fragments arranged by size: 5155 378 231 FspI TGC'GCA Cuts at: 2636 4270 2636 Size: 1634 4130 FspAI rTGC'GCAy Cuts at: 2636 2636 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 2395 4911 1678 Size: 717 2516 2531 Fragments arranged by size: 2531 2516 717 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4405 4437 4479 4511 1373 Size: 32 135 32 2833 32 42 32 2626 Fragments arranged by size: 2833 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5764 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4878 5062 1305 Size: 3573 184 2007 Fragments arranged by size: 3573 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 2402 2615 10 Size: 54 1425 343 546 24 213 3159 Fragments arranged by size: 3159 1425 546 343 213 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2174 2714 3020 3722 4081 4240 Size: 648 981 27 540 306 702 359 159 Cuts at: 4240 518 Size: 2042 Fragments arranged by size: 2042 981 702 648 540 359 306 159 27 MspA1I CmG'CkG Cuts at: 1606 2911 3180 3857 5005 5250 1606 Size: 1305 269 677 1148 245 2120 Fragments arranged by size: 2120 1305 1148 677 269 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NheI G'CTAG_C Cuts at: 1052 2689 1052 Size: 1637 4127 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 2449 3019 3025 3122 4667 1132 Size: 97 1220 570 6 97 1545 2229 Fragments arranged by size: 2229 1545 1220 570 97 97 6 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5764 PfoI T'CCnGG_A Cuts at: 2046 2046 Size: 5764 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PmlI CAC'GTG Cuts at: 2398 2871 2398 Size: 473 5291 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2884 2961 2884 Size: 77 5687 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PspXI vC'TCGA_Gb Cuts at: 2531 2531 Size: 5764 PstI C_TGCA'G Cuts at: 830 2916 3116 830 Size: 2086 200 3478 Fragments arranged by size: 3478 2086 200 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SanDI GG'GwC_CC Cuts at: 2961 2961 Size: 5764 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SfcI C'TryA_G Cuts at: 826 1045 2912 3112 4247 4928 5119 826 Size: 219 1867 200 1135 681 191 1471 Fragments arranged by size: 1867 1471 1135 681 219 200 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2621 2621 Size: 5764 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 2327 2967 3091 1872 Size: 455 640 124 4545 Fragments arranged by size: 4545 640 455 124 SmlI C'TyrA_G Cuts at: 820 1017 1943 2531 2656 2699 3868 4769 5031 Size: 197 926 588 125 43 1169 901 262 Cuts at: 5031 820 Size: 1553 Fragments arranged by size: 1553 1169 926 901 588 262 197 125 43 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 2449 3019 3122 1132 Size: 1317 570 103 3774 Fragments arranged by size: 3774 1317 570 103 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 2537 1517 Size: 1020 4744 StyI C'CwwG_G Cuts at: 513 1065 2004 2067 2409 2506 3203 513 Size: 552 939 63 342 97 697 3074 Fragments arranged by size: 3074 939 697 552 342 97 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3049 3791 3808 3961 4146 4591 325 Size: 1255 1469 742 17 153 185 445 1498 Fragments arranged by size: 1498 1469 1255 742 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2016 4010 5722 Size: 274 80 33 51 1443 39 1994 1712 Cuts at: 5722 96 Size: 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5724 5756 5724 Size: 32 5732 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 2608 2074 Size: 534 5230 XhoI C'TCGA_G Cuts at: 1943 2531 1943 Size: 588 5176 XmaI C'CCGG_G Cuts at: 1870 2325 2965 3089 1870 Size: 455 640 124 4545 Fragments arranged by size: 4545 640 455 124 XmnI GAAnn'nnTTC Cuts at: 2157 3893 2157 Size: 1736 4028 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PsrI PvuII RsrII SacI SacII SapI SexAI SfoI SrfI SwaI