(Linear) (Six-base) MAPSORT of: KIBB7153.seq Check: 7356 from: 1 to: 1059 KIBB7153.orf With 182 enzymes: SgfI * December 27, 2011 16:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 345 732 1059 Size: 345 387 327 Fragments arranged by size: 387 345 327 AflIII A'CryG_T Cuts at: 0 863 1059 Size: 863 196 AleI CACnn'nnGTG Cuts at: 0 709 1059 Size: 709 350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 332 463 1033 1059 Size: 332 131 570 26 Fragments arranged by size: 570 332 131 26 ApoI r'AATT_y Cuts at: 0 837 1059 Size: 837 222 AvaI C'yCGr_G Cuts at: 0 320 526 960 1059 Size: 320 206 434 99 Fragments arranged by size: 434 320 206 99 BanII G_rGCy'C Cuts at: 0 331 1059 Size: 331 728 BbsI GAAGACnn'nnnn_ Cuts at: 0 351 1059 Size: 351 708 BbvCI CC'TCA_GC Cuts at: 0 292 1059 Size: 292 767 BglI GCCn_nnn'nGGC Cuts at: 0 616 1059 Size: 616 443 BmgBI CAC'GTC Cuts at: 0 1025 1059 Size: 1025 34 BmtI G_CTAG'C Cuts at: 0 688 1059 Size: 688 371 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 108 552 1059 Size: 108 444 507 Fragments arranged by size: 507 444 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 975 1021 1059 Size: 975 46 38 Fragments arranged by size: 975 46 38 Bpu10I CC'TnA_GC Cuts at: 0 292 1059 Size: 292 767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 636 679 1059 Size: 636 43 380 Fragments arranged by size: 636 380 43 BsaAI yAC'GTr Cuts at: 0 393 627 866 1059 Size: 393 234 239 193 Fragments arranged by size: 393 239 234 193 BsaHI Gr'CG_yC Cuts at: 0 301 719 1059 Size: 301 418 340 Fragments arranged by size: 418 340 301 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 1059 Size: 328 731 BsiEI CG_ry'CG Cuts at: 0 904 922 1059 Size: 904 18 137 Fragments arranged by size: 904 137 18 BsiHKAI G_wGCw'C Cuts at: 0 164 1059 Size: 164 895 BsmBI CGTCTCn'nnnn_ Cuts at: 0 61 79 831 1059 Size: 61 18 752 228 Fragments arranged by size: 752 228 61 18 Bsp1286I G_dGCh'C Cuts at: 0 164 331 1059 Size: 164 167 728 Fragments arranged by size: 728 167 164 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 890 1059 Size: 890 169 BsrBI CCG'CTC Cuts at: 0 650 1059 Size: 650 409 BsrDI GCAATG_nn' Cuts at: 0 164 206 398 1059 Size: 164 42 192 661 Fragments arranged by size: 661 192 164 42 BssSI C'ACGA_G Cuts at: 0 462 1059 Size: 462 597 BstAPI GCAn_nnn'nTGC Cuts at: 0 570 638 1059 Size: 570 68 421 Fragments arranged by size: 570 421 68 BstEII G'GTnAC_C Cuts at: 0 582 702 1059 Size: 582 120 357 Fragments arranged by size: 582 357 120 BstXI CCAn_nnnn'nTGG Cuts at: 0 144 999 1038 1059 Size: 144 855 39 21 Fragments arranged by size: 855 144 39 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 360 1059 Size: 360 699 BtsI GCAGTG_nn' Cuts at: 0 316 916 1059 Size: 316 600 143 Fragments arranged by size: 600 316 143 EaeI y'GGCC_r Cuts at: 0 224 371 395 608 668 901 919 1059 Size: 224 147 24 213 60 233 18 140 Fragments arranged by size: 233 224 213 147 140 60 24 18 EagI C'GGCC_G Cuts at: 0 901 919 1059 Size: 901 18 140 Fragments arranged by size: 901 140 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 345 732 975 1021 1059 Size: 345 387 243 46 38 Fragments arranged by size: 387 345 243 46 38 EcoO109I rG'GnC_Cy Cuts at: 0 458 879 956 1059 Size: 458 421 77 103 Fragments arranged by size: 458 421 103 77 EcoRI G'AATT_C Cuts at: 0 837 1059 Size: 837 222 FspI TGC'GCA Cuts at: 0 631 1059 Size: 631 428 FspAI rTGC'GCAy Cuts at: 0 631 1059 Size: 631 428 HaeII r_GCGC'y Cuts at: 0 390 1059 Size: 390 669 MscI TGG'CCA Cuts at: 0 373 397 610 1059 Size: 373 24 213 449 Fragments arranged by size: 449 373 213 24 MslI CAynn'nnrTG Cuts at: 0 142 169 709 1015 1059 Size: 142 27 540 306 44 Fragments arranged by size: 540 306 142 44 27 MspA1I CmG'CkG Cuts at: 0 906 1059 Size: 906 153 NheI G'CTAG_C Cuts at: 0 684 1059 Size: 684 375 NspI r_CATG'y Cuts at: 0 444 1014 1020 1059 Size: 444 570 6 39 Fragments arranged by size: 570 444 39 6 PfoI T'CCnGG_A Cuts at: 0 41 1059 Size: 41 1018 PmlI CAC'GTG Cuts at: 0 393 866 1059 Size: 393 473 193 Fragments arranged by size: 473 393 193 PpuMI rG'GwC_Cy Cuts at: 0 879 956 1059 Size: 879 77 103 Fragments arranged by size: 879 103 77 PspXI vC'TCGA_Gb Cuts at: 0 526 1059 Size: 526 533 PstI C_TGCA'G Cuts at: 0 911 1059 Size: 911 148 SanDI GG'GwC_CC Cuts at: 0 956 1059 Size: 956 103 SfcI C'TryA_G Cuts at: 0 907 1059 Size: 907 152 SfiI GGCCn_nnn'nGGCC Cuts at: 0 616 1059 Size: 616 443 SmaI CCC'GGG Cuts at: 0 322 962 1059 Size: 322 640 97 Fragments arranged by size: 640 322 97 SmlI C'TyrA_G Cuts at: 0 526 651 694 1059 Size: 526 125 43 365 Fragments arranged by size: 526 365 125 43 SphI G_CATG'C Cuts at: 0 444 1014 1059 Size: 444 570 45 Fragments arranged by size: 570 444 45 StuI AGG'CCT Cuts at: 0 532 1059 Size: 532 527 StyI C'CwwG_G Cuts at: 0 62 404 501 1059 Size: 62 342 97 558 Fragments arranged by size: 558 342 97 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1044 1059 Size: 1044 15 TatI w'GTAC_w Cuts at: 0 11 1059 Size: 11 1048 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 69 603 1059 Size: 69 534 456 Fragments arranged by size: 534 456 69 XhoI C'TCGA_G Cuts at: 0 526 1059 Size: 526 533 XmaI C'CCGG_G Cuts at: 0 320 960 1059 Size: 320 640 99 Fragments arranged by size: 640 320 99 XmnI GAAnn'nnTTC Cuts at: 0 152 1059 Size: 152 907 Enzymes that do cut: AcuI AflIII AleI AlfI ApoI AvaI BanII BbsI BbvCI BglI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI BstEII BstXI BtgZI BtsI EaeI EagI Eco57MI EcoO109I EcoRI FspI FspAI HaeII MscI MslI MspA1I NheI NspI PfoI PmlI PpuMI PspXI PstI SanDI SfcI SfiI SmaI SmlI SphI StuI StyI TaqII TatI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsiWI BsmI BspEI BspHI BsrFI BsrGI BssHII BstBI BstYI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoRV FalI FseI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI ZraI