################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AvrII BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BlnI BpuAI BsaAI BsaHI BsaI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI Hin1I HindIII HpaI KasI KspI MaeI MaeII MfeI MluI MroI MseI MslI MunI NarI NdeI NheI NotI NruI PacI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SapI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp509I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 553 GGTACC G^GTACC AccI 139 GTCGAC GT^[AC][GT]AC AflIII 135 ACATGT A^C[GA][CT]GT AgeI 119 ACCGGT A^CCGGT AhdI 164 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 553 GGTACC G^GTACC AspEI 164 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 37 CTCGAG C^[CT]CG[GA]G AviII 689 TGCGCA TGC^GCA BalI 662 TGGCCA TGG^CCA BamHI 71 GGATCC G^GATCC BcgI 180 GCACCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 600 GCTCAGC GC^T[ACGT]AGC Bpu1102I 600 GCTCAGC GC^T[ACGT]AGC BsaBI 72 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 825 CTCCTC GAGGAG(10/8) BsiEI 117 CGACCG CG[GA][CT]^CG BsmAI 571 GAGAC GTCTC(1/5) BsoBI 37 CTCGAG C^[CT]CG[GA]G BspLU11I 135 ACATGT A^CATGT BspMI 503 ACCTGC ACCTGC(4/8) BstBI 188 TTCGAA TT^CGAA CelII 600 GCTCAGC GC^T[ACGT]AGC DsaI 410 CCATGG C^C[GA][CT]GG Eam1105I 164 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 102 GAAGAG CTCTTC(1/4) EcoNI 906 CCTGTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 410 CCATGG C^C[AT][AT]GG EcoT22I 462 ATGCAT ATGCA^T FauI 484 CCCGC CCCGC(4/6) FspI 689 TGCGCA TGC^GCA HaeII 314 AGCGCC [GA]GCGC^[CT] HincII 139 GTCGAC GT[CT]^[GA]AC HindII 139 GTCGAC GT[CT]^[GA]AC KpnI 553 GGTACC GGTAC^C Ksp632I 102 GAAGAG CTCTTC(1/4) MamI 72 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 662 TGGCCA TGG^CCA MscI 662 TGGCCA TGG^CCA NaeI 753 GCCGGC GCC^GGC NcoI 410 CCATGG C^CATGG NgoMIV 753 GCCGGC G^CCGGC NsiI 462 ATGCAT ATGCA^T NspV 188 TTCGAA TT^CGAA PaeR7I 37 CTCGAG C^TCGAG PinAI 119 ACCGGT A^CCGGT PshAI 6 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 396 CAGCTG CAG^CTG SacI 598 GAGCTC GAGCT^C SalI 139 GTCGAC G^TCGAC SfuI 188 TTCGAA TT^CGAA StyI 410 CCATGG C^C[AT][AT]GG XcmI 953 CCAAAGAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 37 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 478 CGCG CG^CG 518 CGCG AspHI 499 GAGCAC G[AT]GC[AT]^C 598 GAGCTC AspI 25 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 475 GACCGCGTC BanII 284 GAGCCC G[GA]GC[CT]^C 598 GAGCTC BglII 41 AGATCT A^GATCT 404 AGATCT BsaWI 68 ACCGGA [AT]^CCGG[AT] 119 ACCGGT BsgI 391 CTGCAC GTGCAG(16/14) 633 CTGCAC BsiHKAI 499 GAGCAC G[AT]GC[AT]^C 598 GAGCTC BsmI 460 GAATGC GAATGC(1/-1) 749 GAATGC BsrFI 119 ACCGGT [GA]^CCGG[CT] 753 GCCGGC BsrI 968 ACTGG ACTGG(1/-1) 261 CCAGT BstUI 478 CGCG CG^CG 518 CGCG Cfr10I 119 ACCGGT [GA]^CCGG[CT] 753 GCCGGC EaeI 332 TGGCCG [CT]^GGCC[GA] 662 TGGCCA MspA1I 96 CCGCTG C[AC]G^C[GT]G 396 CAGCTG MvnI 478 CGCG CG^CG 518 CGCG NspI 135 ACATGT [GA]CATG^[CT] 327 GCATGT PflFI 25 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 475 GACCGCGTC PflMI 410 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 924 CCACTGTATGG TspRI 813 CACTG CA[GC]TG[ACGT][ACGT]^ 925 CACTG Tth111I 25 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 475 GACCGCGTC Van91I 410 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 924 CCACTGTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I TaqI TseI Tsp45I TthHB8I XhoII