(Circular) (Six-base) MAPSORT of: pFN21AE3987.seq Check: 9215 from: 1 to: 5683 pFN21AE3987 5683 bp With 182 enzymes: SgfI * March 28, 2012 11:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2517 2517 Size: 5683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2145 3026 1937 Size: 208 881 4594 Fragments arranged by size: 4594 881 208 Acc65I G'GTAC_C Cuts at: 2558 3004 2558 Size: 446 5237 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 3746 5130 5398 1632 Size: 704 1410 1384 268 1917 Fragments arranged by size: 1917 1410 1384 704 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 2140 4582 5559 1203 Size: 937 2442 977 1327 Fragments arranged by size: 2442 1327 977 937 AgeI A'CCGG_T Cuts at: 2124 4514 2124 Size: 2390 3293 AhdI GACnn_n'nnGTC Cuts at: 2174 4412 2174 Size: 2238 3445 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5683 AlwNI CAG_nnn'CTG Cuts at: 2094 2518 2878 3426 4998 2094 Size: 424 360 548 1572 2779 Fragments arranged by size: 2779 1572 548 424 360 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2992 3218 3370 5479 1493 Size: 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2042 3008 1870 Size: 73 99 966 4545 Fragments arranged by size: 4545 966 99 73 BamHI G'GATC_C Cuts at: 2076 3013 3380 2076 Size: 937 367 4379 Fragments arranged by size: 4379 937 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2184 2353 2558 3004 4459 Size: 325 217 627 397 169 205 446 1455 Cuts at: 4459 618 Size: 1842 Fragments arranged by size: 1842 1455 627 446 397 325 217 205 169 BanII G_rGCy'C Cuts at: 1391 2293 2607 3002 1391 Size: 902 314 395 4072 Fragments arranged by size: 4072 902 395 314 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2174 2208 3874 3908 1903 Size: 34 237 34 1666 34 3678 Fragments arranged by size: 3678 1666 237 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 1548 Size: 5683 BfrBI ATG'CAT Cuts at: 2469 2469 Size: 5683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4294 136 Size: 107 122 71 631 605 2622 1525 Fragments arranged by size: 2622 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2046 2409 5678 2046 Size: 363 3269 2051 Fragments arranged by size: 3269 2051 363 BlpI GC'TnA_GC Cuts at: 2606 3100 2606 Size: 494 5189 Bme1580I G_kGCm'C Cuts at: 1790 2309 2358 3744 4900 1790 Size: 519 49 1386 1156 2573 Fragments arranged by size: 2573 1386 1156 519 49 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2260 3414 4372 Size: 416 53 83 399 24 843 1154 958 Cuts at: 4372 442 Size: 1753 Fragments arranged by size: 1753 1154 958 843 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2068 2068 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2071 2411 2768 2786 4343 1239 Size: 832 340 357 18 1557 2579 Fragments arranged by size: 2579 1557 832 357 340 18 Bpu10I CC'TnA_GC Cuts at: 2345 2345 Size: 5683 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2401 2645 2885 3808 4673 4971 2401 Size: 244 240 923 865 298 3113 Fragments arranged by size: 3113 923 865 298 244 240 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4346 882 Size: 617 2847 2219 Fragments arranged by size: 2847 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 2081 5677 2081 Size: 3596 2087 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3490 3872 4507 275 Size: 53 83 186 936 1957 382 635 1451 Fragments arranged by size: 1957 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2073 2124 4116 4514 4788 4935 5346 2073 Size: 51 1992 398 274 147 411 2410 Fragments arranged by size: 2410 1992 411 398 274 147 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2759 2789 1178 Size: 30 165 30 1356 30 4072 Fragments arranged by size: 4072 1356 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2821 1186 Size: 1635 4048 BseYI C'CCAG_C Cuts at: 2372 2551 2632 2878 4886 5382 2372 Size: 179 81 246 2008 496 2673 Fragments arranged by size: 2673 2008 496 246 179 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 2623 1163 Size: 573 645 242 4223 Fragments arranged by size: 4223 645 573 242 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2125 3133 3894 4922 1540 Size: 275 186 124 1008 761 1028 2301 Fragments arranged by size: 2301 1028 1008 761 275 186 124 BsiHKAI G_wGCw'C Cuts at: 2508 2607 3744 3829 4900 2508 Size: 99 1137 85 1071 3291 Fragments arranged by size: 3291 1137 1071 99 85 BsmI GAATG_Cn' Cuts at: 2471 2760 3194 3287 2471 Size: 289 434 93 4867 Fragments arranged by size: 4867 434 289 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2293 2309 2358 2508 2607 3002 3744 Size: 399 503 16 49 150 99 395 742 Cuts at: 3744 3829 4900 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 503 399 395 150 99 85 49 16 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 3038 844 Size: 1673 521 3489 Fragments arranged by size: 3489 1673 521 BsrBI CCG'CTC Cuts at: 1394 1823 3570 1394 Size: 429 1747 3507 Fragments arranged by size: 3507 1747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2124 2758 4327 4514 1700 Size: 110 314 634 1569 187 2869 Fragments arranged by size: 2869 1569 634 314 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 2194 4487 2194 Size: 2293 3390 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5683 BstYI r'GATC_y Cuts at: 1972 2046 2076 2409 2700 2907 3013 3380 3765 Size: 74 30 333 291 207 106 367 385 Cuts at: 3765 3782 5234 5320 5332 5678 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 333 291 207 106 86 74 30 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2415 513 Size: 552 939 411 3781 Fragments arranged by size: 3781 939 552 411 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 3199 3993 4013 3199 Size: 794 20 4869 Fragments arranged by size: 4869 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2337 2667 3130 Size: 54 1425 50 275 18 507 330 463 Cuts at: 3130 4019 5648 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 507 463 330 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 3613 1353 Size: 749 1511 3423 Fragments arranged by size: 3423 1511 749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2915 4270 4644 4790 387 Size: 1064 138 1326 1355 374 146 1280 Fragments arranged by size: 1355 1326 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2605 2605 Size: 5683 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2336 2411 2768 2786 3746 4343 Size: 393 439 265 75 357 18 960 597 Cuts at: 4343 5130 5398 1239 Size: 787 268 1524 Fragments arranged by size: 1524 960 787 597 439 393 357 268 265 75 18 EcoNI CCTnn'n_nnAGG Cuts at: 2915 2915 Size: 5683 EcoO109I rG'GnC_Cy Cuts at: 2260 2385 2440 2515 2719 2786 2260 Size: 125 55 75 204 67 5157 Fragments arranged by size: 5157 204 125 75 67 55 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FspI TGC'GCA Cuts at: 2696 4189 2696 Size: 1493 4190 HaeII r_GCGC'y Cuts at: 1678 2323 4830 1678 Size: 645 2507 2531 Fragments arranged by size: 2531 2507 645 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2160 2192 4324 4356 4398 Size: 32 135 32 588 32 2132 32 42 Cuts at: 4398 4430 1373 Size: 32 2626 Fragments arranged by size: 2626 2132 588 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 3027 3273 669 Size: 988 281 208 881 246 3079 Fragments arranged by size: 3079 988 881 281 246 208 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 2562 3008 2562 Size: 446 5237 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4797 4981 1305 Size: 3492 184 2007 Fragments arranged by size: 3492 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 10 Size: 54 1425 343 837 3024 Fragments arranged by size: 3024 1425 837 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3641 4000 4159 518 Size: 648 2475 359 159 2042 Fragments arranged by size: 2475 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2103 2403 3099 3776 4924 5169 1606 Size: 497 300 696 677 1148 245 2120 Fragments arranged by size: 2120 1148 696 677 497 300 245 NaeI GCC'GGC Cuts at: 2760 2760 Size: 5683 NcoI C'CATG_G Cuts at: 513 1065 2004 2415 513 Size: 552 939 411 3781 Fragments arranged by size: 3781 939 552 411 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NgoMIV G'CCGG_C Cuts at: 2758 2758 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NsiI A_TGCA'T Cuts at: 2471 2471 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 2144 2336 3041 4586 1132 Size: 97 915 192 705 1545 2229 Fragments arranged by size: 2229 1545 915 705 192 97 PasI CC'CwG_GG Cuts at: 1348 2293 1348 Size: 945 4738 PciI A'CATG_T Cuts at: 2140 4582 2140 Size: 2442 3241 PflMI CCAn_nnn'nTGG Cuts at: 1108 2421 2935 1108 Size: 1313 514 3856 Fragments arranged by size: 3856 1313 514 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2385 2440 2786 2385 Size: 55 346 5282 Fragments arranged by size: 5282 346 55 PshAI GACnn'nnGTC Cuts at: 1613 2015 1613 Size: 402 5281 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PstI C_TGCA'G Cuts at: 830 2514 2601 2619 2784 2874 3035 830 Size: 1684 87 18 165 90 161 3478 Fragments arranged by size: 3478 1684 165 161 90 87 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2403 2403 Size: 5683 SacI G_AGCT'C Cuts at: 2607 2607 Size: 5683 SalI G'TCGA_C Cuts at: 1936 2144 3025 1936 Size: 208 881 4594 Fragments arranged by size: 4594 881 208 SanDI GG'GwC_CC Cuts at: 2786 2786 Size: 5683 SbfI CC_TGCA'GG Cuts at: 2514 2601 2784 3035 2514 Size: 87 183 251 5162 Fragments arranged by size: 5162 251 183 87 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 2510 2597 2615 2780 2870 3031 4166 Size: 219 1465 87 18 165 90 161 1135 Cuts at: 4166 4847 5038 826 Size: 681 191 1471 Fragments arranged by size: 1471 1465 1135 681 219 191 165 161 90 87 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 3010 1872 Size: 1138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2042 2380 2624 2864 3787 4688 Size: 197 926 99 338 244 240 923 901 Cuts at: 4688 4950 820 Size: 262 1553 Fragments arranged by size: 1553 926 923 901 338 262 244 240 197 99 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5683 StyI C'CwwG_G Cuts at: 513 1065 2004 2415 3122 513 Size: 552 939 411 707 3074 Fragments arranged by size: 3074 939 707 552 411 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2023 2222 2543 2588 3710 3727 3880 Size: 1255 443 199 321 45 1122 17 153 Cuts at: 3880 4065 4510 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1122 445 443 321 199 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3929 5641 96 Size: 274 80 33 51 1443 1952 1712 138 Fragments arranged by size: 1952 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 2759 2791 5643 5675 2111 Size: 32 616 32 2852 32 2119 Fragments arranged by size: 2852 2119 616 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2033 2483 1316 Size: 717 450 4516 Fragments arranged by size: 4516 717 450 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2965 2011 Size: 954 4729 XhoI C'TCGA_G Cuts at: 1943 2042 1943 Size: 99 5584 XmaI C'CCGG_G Cuts at: 1870 3008 1870 Size: 1138 4545 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI