################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 1394 GTAGAC GT^[AC][GT]AC AflII 397 CTTAAG C^TTAAG AflIII 978 ACATGT A^C[GA][CT]GT Alw44I 472 GTGCAC G^TGCAC AlwI 863 GATCC GGATC(4/5) ApaLI 472 GTGCAC G^TGCAC AspHI 472 GTGCAC G[AT]GC[AT]^C AvaI 1047 CTCGGG C^[CT]CG[GA]G BalI 1026 TGGCCA TGG^CCA BbvI 4 GCAGC GCAGC(8/12) BciVI 314 GGATAC GTATCC(6/5) BclI 891 TGATCA T^GATCA BfrI 397 CTTAAG C^TTAAG BmyI 472 GTGCAC G[AGT]GC[ACT]^C BsaWI 1330 ACCGGA [AT]^CCGG[AT] BseRI 157 GAGGAG GAGGAG(10/8) BsiHKAI 472 GTGCAC G[AT]GC[AT]^C BsmFI 18 GTCCC GGGAC(10/14) BsmI 1432 GAATGC GAATGC(1/-1) BsoBI 1047 CTCGGG C^[CT]CG[GA]G Bsp1286I 472 GTGCAC G[AGT]GC[ACT]^C Bsp1407I 555 TGTACA T^GTACA BspLU11I 978 ACATGT A^CATGT BspMI 1316 ACCTGC ACCTGC(4/8) BsrGI 555 TGTACA T^GTACA BstXI 154 CCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 862 AGATCC [GA]^GATC[CT] Cac8I 628 GCAAGC GC[ACGT]^[ACGT]GC Cfr13I 1050 GGGCC G^G[ACGT]CC DsaI 21 CCGTGG C^C[GA][CT]GG EaeI 1026 TGGCCA [CT]^GGCC[GA] FbaI 891 TGATCA T^GATCA Fnu4HI 4 GCAGC GC^[ACGT]GC HapII 1331 CCGG C^CGG HpaII 1331 CCGG C^CGG ItaI 4 GCAGC GC^[ACGT]GC MaeII 124 ACGT A^CGT MflI 862 AGATCC [GA]^GATC[CT] MluNI 1026 TGGCCA TGG^CCA MscI 1026 TGGCCA TGG^CCA MspI 1331 CCGG C^CGG MwoI 697 GCTGTCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 643 CATATG CA^TATG NlaIV 39 GGAACC GG[ACGT]^[ACGT]CC NspI 978 ACATGT [GA]CATG^[CT] Sau96I 1050 GGGCC G^G[ACGT]CC ScaI 1043 AGTACT AGT^ACT SfaNI 207 GATGC GCATC(5/9) SmlI 397 CTTAAG C^T[CT][GA]AG SspBI 555 TGTACA T^GTACA SspI 303 AATATT AAT^ATT TseI 4 GCAGC G^C[AT]GC XhoII 862 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AluI 696 AGCT AG^CT 705 AGCT BbsI 1197 GAAGAC GAAGAC(2/6) 376 GTCTTC BfaI 257 CTAG C^TAG 956 CTAG BpmI 542 CTCCAG CTGGAG(16/14) 211 CTGGAG BpuAI 1197 GAAGAC GAAGAC(2/6) 376 GTCTTC BsaJI 21 CCGTGG C^C[ACGT][ACGT]GG 35 CCAGGG EcoRI 1154 GAATTC G^AATTC 1406 GAATTC HphI 149 TCACC GGTGA(8/7) 1314 TCACC MaeI 257 CTAG C^TAG 956 CTAG MslI 655 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 923 CACACAAGTG SfcI 195 CTACAG C^T[GA][CT]AG 333 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AcsI AfaI ApoI Asp700I BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII FokI HaeIII HinfI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII RsaI Sau3AI ScrFI TfiI Tru9I Tsp45I Tsp509I TspRI XmnI