(Circular) (Six-base) MAPSORT of: pFN21AE1150.seq Check: 1054 from: 1 to: 6163 pFN21AE1150 6163 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3330 3330 Size: 6163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3400 3506 1937 Size: 1463 106 4594 Fragments arranged by size: 4594 1463 106 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2368 4226 5610 5878 1632 Size: 635 101 1858 1384 268 1917 Fragments arranged by size: 1917 1858 1384 635 268 101 AflII C'TTAA_G Cuts at: 820 1017 2402 820 Size: 197 1385 4581 Fragments arranged by size: 4581 1385 197 AflIII A'CryG_T Cuts at: 1203 2983 5062 6039 1203 Size: 1780 2079 977 1327 Fragments arranged by size: 2079 1780 1327 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 4892 4892 Size: 6163 AleI CACnn'nnGTG Cuts at: 1166 2932 1166 Size: 1766 4397 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AlwNI CAG_nnn'CTG Cuts at: 3906 5478 3906 Size: 1572 4591 ApaLI G'TGCA_C Cuts at: 2477 4220 5376 2477 Size: 1743 1156 3264 Fragments arranged by size: 3264 1743 1156 ApoI r'AATT_y Cuts at: 1493 2513 2681 2878 2907 3159 3411 3472 3698 Size: 1020 168 197 29 252 252 61 226 Cuts at: 3698 3850 5959 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1020 252 252 226 197 168 152 61 29 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 3052 3488 1870 Size: 73 1109 436 4545 Fragments arranged by size: 4545 1109 436 73 BamHI G'GATC_C Cuts at: 3493 3860 3493 Size: 367 5796 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3484 4939 618 Size: 325 217 627 1697 1455 1842 Fragments arranged by size: 1842 1697 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3482 1391 Size: 2091 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2374 3209 928 Size: 971 475 835 3882 Fragments arranged by size: 3882 971 835 475 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4354 4388 1903 Size: 34 2417 34 3678 Fragments arranged by size: 3678 2417 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2313 4055 5265 2313 Size: 1742 1210 3211 Fragments arranged by size: 3211 1742 1210 BclI T'GATC_A Cuts at: 1548 2896 1548 Size: 1348 4815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4774 136 Size: 107 122 71 631 605 3102 1525 Fragments arranged by size: 3102 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 3580 3580 Size: 6163 Bme1580I G_kGCm'C Cuts at: 1790 2481 4224 5380 1790 Size: 691 1743 1156 2573 Fragments arranged by size: 2573 1743 1156 691 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3429 3894 4852 Size: 416 53 83 399 24 2012 465 958 Cuts at: 4852 442 Size: 1753 Fragments arranged by size: 2012 1753 958 465 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 2532 4823 1239 Size: 998 295 2291 2579 Fragments arranged by size: 2579 2291 998 295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4288 5153 5451 4288 Size: 865 298 5000 Fragments arranged by size: 5000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4826 882 Size: 617 3327 2219 Fragments arranged by size: 3327 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3954 493 Size: 711 2750 2702 Fragments arranged by size: 2750 2702 711 BsaBI GATnn'nnATC Cuts at: 6157 6157 Size: 6163 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3970 4352 4987 275 Size: 53 83 186 936 2437 382 635 1451 Fragments arranged by size: 2437 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3335 4596 4994 5268 5415 5826 3335 Size: 1261 398 274 147 411 3672 Fragments arranged by size: 3672 1261 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2530 2560 1178 Size: 30 165 30 1127 30 4781 Fragments arranged by size: 4781 1127 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 1186 Size: 991 5172 BseYI C'CCAG_C Cuts at: 5366 5862 5366 Size: 496 5667 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5590 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3613 4374 5402 1540 Size: 275 186 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2481 4224 4309 5380 2481 Size: 1743 85 1071 3264 Fragments arranged by size: 3264 1743 1071 85 BsmI GAATG_Cn' Cuts at: 3443 3674 3767 3443 Size: 231 93 5839 Fragments arranged by size: 5839 231 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2481 3482 4224 4309 5380 1391 Size: 399 691 1001 742 85 1071 2174 Fragments arranged by size: 2174 1071 1001 742 691 399 85 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3330 3518 844 Size: 2486 188 3489 Fragments arranged by size: 3489 2486 188 BsrBI CCG'CTC Cuts at: 1394 1823 4050 1394 Size: 429 2227 3507 Fragments arranged by size: 3507 2227 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4658 4832 65 Size: 1183 3410 174 1396 Fragments arranged by size: 3410 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4807 4994 1700 Size: 110 2997 187 2869 Fragments arranged by size: 2997 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2560 96 Size: 2464 3699 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6163 BssSI C'ACGA_G Cuts at: 4223 5235 4223 Size: 1012 5151 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6163 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstXI CCAn_nnnn'nTGG Cuts at: 2166 2166 Size: 6163 BstYI r'GATC_y Cuts at: 1972 2867 3493 3860 4245 4262 5714 5800 5812 Size: 895 626 367 385 17 1452 86 12 Cuts at: 5812 6158 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 895 626 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2026 513 Size: 552 939 22 4650 Fragments arranged by size: 4650 939 552 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5424 Fragments arranged by size: 5424 683 56 BtsI GCAGTG_nn' Cuts at: 2735 3679 4473 4493 2735 Size: 944 794 20 4405 Fragments arranged by size: 4405 944 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3262 3297 529 Size: 35 2698 35 3395 Fragments arranged by size: 3395 2698 35 35 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3031 3610 4499 Size: 54 1425 50 275 18 1201 579 889 Cuts at: 4499 6128 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1201 889 579 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3610 1537 Size: 275 1798 4090 Fragments arranged by size: 4090 1798 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4093 1353 Size: 2740 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4750 5124 5270 387 Size: 1064 138 3161 374 146 1280 Fragments arranged by size: 3161 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2267 2368 2532 4226 4823 5610 Size: 393 605 30 101 164 1694 597 787 Cuts at: 5610 5878 1239 Size: 268 1524 Fragments arranged by size: 1694 1524 787 605 597 393 268 164 101 30 EcoRI G'AATT_C Cuts at: 3159 3411 3472 3850 3159 Size: 252 61 378 5472 Fragments arranged by size: 5472 378 252 61 FspI TGC'GCA Cuts at: 4669 4669 Size: 6163 HaeII r_GCGC'y Cuts at: 1678 5310 1678 Size: 3632 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2337 2369 4804 4836 4878 Size: 32 135 32 765 32 2435 32 42 Cuts at: 4878 4910 1373 Size: 32 2626 Fragments arranged by size: 2626 2435 765 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3507 3753 669 Size: 988 281 1569 246 3079 Fragments arranged by size: 3079 1569 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6163 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5277 5461 1305 Size: 3972 184 2007 Fragments arranged by size: 3972 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3033 10 Size: 54 1425 343 1201 3140 Fragments arranged by size: 3140 1425 1201 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2664 2932 4121 4480 4639 518 Size: 648 1498 268 1189 359 159 2042 Fragments arranged by size: 2042 1498 1189 648 359 268 159 MspA1I CmG'CkG Cuts at: 1606 3579 4256 5404 5649 1606 Size: 1973 677 1148 245 2120 Fragments arranged by size: 2120 1973 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4672 Fragments arranged by size: 4672 939 552 NdeI CA'TA_TG Cuts at: 387 2649 387 Size: 2262 3901 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 2987 3521 5066 1132 Size: 97 1758 534 1545 2229 Fragments arranged by size: 2229 1758 1545 534 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6163 PciI A'CATG_T Cuts at: 2983 5062 2983 Size: 2079 4084 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6163 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2222 2254 4232 4264 5745 5777 2222 Size: 32 1978 32 1481 32 2608 Fragments arranged by size: 2608 1978 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PstI C_TGCA'G Cuts at: 830 3515 830 Size: 2685 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SbfI CC_TGCA'GG Cuts at: 3515 3515 Size: 6163 ScaI AGT'ACT Cuts at: 3050 4411 6123 3050 Size: 1361 1712 3090 Fragments arranged by size: 3090 1712 1361 SfcI C'TryA_G Cuts at: 826 1045 2200 2338 3511 4646 5327 5518 826 Size: 219 1155 138 1173 1135 681 191 1471 Fragments arranged by size: 1471 1173 1155 1135 681 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 3490 1872 Size: 1618 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2402 4267 5168 5430 820 Size: 197 926 459 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 459 262 197 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 2310 4087 5 Size: 47 2258 1777 2081 Fragments arranged by size: 2258 2081 1777 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6163 StyI C'CwwG_G Cuts at: 513 1065 2004 3602 513 Size: 552 939 1598 3074 Fragments arranged by size: 3074 1598 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4190 4207 4360 4545 4990 325 Size: 1255 2610 17 153 185 445 1498 Fragments arranged by size: 2610 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2560 2936 3048 Size: 274 80 33 51 1443 583 376 112 Cuts at: 3048 4409 6121 96 Size: 1361 1712 138 Fragments arranged by size: 1712 1443 1361 583 376 274 138 112 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2341 2373 6123 6155 2341 Size: 32 3750 32 2349 Fragments arranged by size: 3750 2349 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6163 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6163 XmaI C'CCGG_G Cuts at: 1870 3488 1870 Size: 1618 4545 XmnI GAAnn'nnTTC Cuts at: 2786 2954 3122 3206 3347 3411 4292 2786 Size: 168 168 84 141 64 881 4657 Fragments arranged by size: 4657 881 168 168 141 84 64 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI