(Circular) (Six-base) MAPSORT of: pFN21AE2375.seq Check: 6779 from: 1 to: 6895 pFN21AE2375 6895 bp With 182 enzymes: SgfI * October 20, 2011 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4705 278 Size: 53 83 186 936 3169 2468 Fragments arranged by size: 3169 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4238 1937 Size: 2301 4594 Acc65I G'GTAC_C Cuts at: 4216 4216 Size: 6895 AclI AA'CG_TT Cuts at: 1563 5022 5395 1563 Size: 3459 373 3063 Fragments arranged by size: 3459 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2346 2684 3164 4958 6342 6610 1632 Size: 714 338 480 1794 1384 268 1917 Fragments arranged by size: 1917 1794 1384 714 480 338 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6698 AflIII A'CryG_T Cuts at: 1203 5794 6771 1203 Size: 4591 977 1327 Fragments arranged by size: 4591 1327 977 AgeI A'CCGG_T Cuts at: 5726 5726 Size: 6895 AhdI GACnn_n'nnGTC Cuts at: 5624 5624 Size: 6895 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6895 AlwNI CAG_nnn'CTG Cuts at: 4638 6210 4638 Size: 1572 5323 ApaI G_GGCC'C Cuts at: 2111 3060 3380 3563 3641 3785 3797 2111 Size: 949 320 183 78 144 12 5209 Fragments arranged by size: 5209 949 320 183 144 78 12 ApaLI G'TGCA_C Cuts at: 4952 6108 4952 Size: 1156 5739 ApoI r'AATT_y Cuts at: 1493 2357 4019 4204 4430 4582 6691 1493 Size: 864 1662 185 226 152 2109 1697 Fragments arranged by size: 2109 1697 1662 864 226 185 152 AscI GG'CGCG_CC Cuts at: 2073 2073 Size: 6895 AseI AT'TA_AT Cuts at: 160 1032 5449 160 Size: 872 4417 1606 Fragments arranged by size: 4417 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6895 AvaI C'yCGr_G Cuts at: 1870 1943 3236 3731 3770 4220 1870 Size: 73 1293 495 39 450 4545 Fragments arranged by size: 4545 1293 495 450 73 39 BamHI G'GATC_C Cuts at: 4225 4592 4225 Size: 367 6528 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3242 3464 3601 3680 3719 Size: 325 217 627 1455 222 137 79 39 Cuts at: 3719 3758 3851 4216 5671 618 Size: 39 93 365 1455 1842 Fragments arranged by size: 1842 1455 1455 627 365 325 222 217 137 93 79 39 39 BanII G_rGCy'C Cuts at: 1391 2104 2111 2313 3060 3299 3341 3359 3380 Size: 713 7 202 747 239 42 18 21 Cuts at: 3380 3542 3563 3641 3785 3797 4214 1391 Size: 162 21 78 144 12 417 4072 Fragments arranged by size: 4072 747 713 417 239 202 162 144 78 42 21 21 18 12 7 BbeI G_GCGC'C Cuts at: 3684 3723 3684 Size: 39 6856 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2428 2611 928 Size: 971 529 183 5212 Fragments arranged by size: 5212 971 529 183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3460 3494 5086 5120 1903 Size: 34 1523 34 1592 34 3678 Fragments arranged by size: 3678 1592 1523 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2561 4787 5997 2561 Size: 2226 1210 3459 Fragments arranged by size: 3459 2226 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6895 BfrBI ATG'CAT Cuts at: 2591 2591 Size: 6895 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3384 3956 Size: 107 122 71 631 605 337 1375 572 Cuts at: 3956 5506 136 Size: 1550 1525 Fragments arranged by size: 1550 1525 1375 631 605 572 337 122 107 71 BglII A'GATC_T Cuts at: 6890 6890 Size: 6895 BlpI GC'TnA_GC Cuts at: 4312 4312 Size: 6895 Bme1580I G_kGCm'C Cuts at: 1790 2111 3060 3380 3563 3606 3641 3785 3797 Size: 321 949 320 183 43 35 144 12 Cuts at: 3797 4956 6112 1790 Size: 1159 1156 2573 Fragments arranged by size: 2573 1159 1156 949 321 320 183 144 43 35 12 BmgBI CAC'GTC Cuts at: 1318 2830 1318 Size: 1512 5383 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2541 4626 5584 Size: 416 53 83 399 24 1124 2085 958 Cuts at: 5584 442 Size: 1753 Fragments arranged by size: 2085 1753 1124 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6895 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3388 3647 3689 3713 3728 3767 3948 5555 Size: 2149 259 42 24 15 39 181 1607 Cuts at: 5555 1239 Size: 2579 Fragments arranged by size: 2579 2149 1607 259 181 42 39 24 15 Bpu10I CC'TnA_GC Cuts at: 2877 3584 2877 Size: 707 6188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3506 5020 5885 6183 3506 Size: 1514 865 298 4218 Fragments arranged by size: 4218 1514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5558 882 Size: 617 4059 2219 Fragments arranged by size: 4059 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2305 4039 4686 493 Size: 711 1101 1734 647 2702 Fragments arranged by size: 2702 1734 1101 711 647 BsaBI GATnn'nnATC Cuts at: 2537 2955 3094 6889 2537 Size: 418 139 3795 2543 Fragments arranged by size: 3795 2543 418 139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3681 3720 4702 5084 Size: 53 83 186 936 2148 39 982 382 Cuts at: 5084 5719 275 Size: 635 1451 Fragments arranged by size: 2148 1451 982 936 635 382 186 83 53 39 BsaWI w'CCGG_w Cuts at: 5328 5726 6000 6147 6558 5328 Size: 398 274 147 411 5665 Fragments arranged by size: 5665 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3180 3210 3386 3416 1178 Size: 30 165 30 1777 30 176 30 4657 Fragments arranged by size: 4657 1777 176 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2187 3165 3189 1186 Size: 1001 978 24 4892 Fragments arranged by size: 4892 1001 978 24 BseYI C'CCAG_C Cuts at: 3382 6098 6594 3382 Size: 2716 496 3683 Fragments arranged by size: 3683 2716 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6322 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4345 5106 6134 1540 Size: 275 186 1510 834 761 1028 2301 Fragments arranged by size: 2301 1510 1028 834 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 4956 5041 6112 2313 Size: 2643 85 1071 3096 Fragments arranged by size: 3096 2643 1071 85 BsmI GAATG_Cn' Cuts at: 4406 4499 4406 Size: 93 6802 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2111 2313 3060 3299 3341 3359 Size: 399 314 7 202 747 239 42 18 Cuts at: 3359 3380 3542 3563 3606 3641 3785 3797 4214 Size: 21 162 21 43 35 144 12 417 Cuts at: 4214 4956 5041 6112 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 747 742 417 399 314 239 202 162 144 85 43 42 35 21 21 18 12 7 BspEI T'CCGG_A Cuts at: 6558 6558 Size: 6895 BspHI T'CATG_A Cuts at: 4784 6514 4784 Size: 1730 5165 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2140 3599 3899 4250 844 Size: 1296 1459 300 351 3489 Fragments arranged by size: 3489 1459 1296 351 300 BsrBI CCG'CTC Cuts at: 1394 1823 2070 2091 2418 3500 3697 4782 1394 Size: 429 247 21 327 1082 197 1085 3507 Fragments arranged by size: 3507 1085 1082 429 327 247 197 21 BsrDI GCAATG_nn' Cuts at: 65 1248 2478 3159 5390 5564 65 Size: 1183 1230 681 2231 174 1396 Fragments arranged by size: 2231 1396 1230 1183 681 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3245 3467 3662 5539 5726 1700 Size: 110 1435 222 195 1877 187 2869 Fragments arranged by size: 2869 1877 1435 222 195 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6895 BssHII G'CGCG_C Cuts at: 1925 2073 1925 Size: 148 6747 BssSI C'ACGA_G Cuts at: 4955 5967 4955 Size: 1012 5883 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6895 BstBI TT'CG_AA Cuts at: 4177 5699 4177 Size: 1522 5373 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6895 BstXI CCAn_nnnn'nTGG Cuts at: 2906 3667 3691 3877 3941 2906 Size: 761 24 186 64 5860 Fragments arranged by size: 5860 761 186 64 24 BstYI r'GATC_y Cuts at: 1972 2443 3095 4225 4592 4977 4994 6446 6532 Size: 471 652 1130 367 385 17 1452 86 Cuts at: 6532 6544 6890 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1130 652 471 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2112 2112 Size: 6895 BtgI C'CryG_G Cuts at: 513 1065 2004 2899 3277 3342 3429 3789 3834 Size: 552 939 895 378 65 87 360 45 Cuts at: 3834 513 Size: 3574 Fragments arranged by size: 3574 939 895 552 378 360 87 65 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6156 Fragments arranged by size: 6156 683 56 BtsI GCAGTG_nn' Cuts at: 4411 5205 5225 4411 Size: 794 20 6081 Fragments arranged by size: 6081 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6895 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4066 4101 529 Size: 35 3502 35 3323 Fragments arranged by size: 3502 3323 35 35 DraI TTT'AAA Cuts at: 4199 4199 Size: 6895 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5902 809 Size: 507 4586 1802 Fragments arranged by size: 4586 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2800 3495 3657 Size: 54 1425 50 275 18 970 695 162 Cuts at: 3657 3831 3948 4033 4116 4159 4342 5231 6860 Size: 174 117 85 83 43 183 889 1629 Cuts at: 6860 8 Size: 43 Fragments arranged by size: 1629 1425 970 889 695 275 183 174 162 117 85 83 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4342 1537 Size: 275 2530 4090 Fragments arranged by size: 4090 2530 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2141 2364 3140 4825 1353 Size: 705 83 223 776 1685 3423 Fragments arranged by size: 3423 1685 776 705 223 83 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 5482 5856 6002 387 Size: 1064 138 456 3437 374 146 1280 Fragments arranged by size: 3437 1280 1064 456 374 146 138 EcoICRI GAG'CTC Cuts at: 2311 2311 Size: 6895 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2346 2684 3164 3388 3647 3689 3713 Size: 393 714 338 480 224 259 42 24 Cuts at: 3713 3728 3767 3948 4958 5555 6342 6610 1239 Size: 15 39 181 1010 597 787 268 1524 Fragments arranged by size: 1524 1010 787 714 597 480 393 338 268 259 224 181 42 39 24 15 EcoO109I rG'GnC_Cy Cuts at: 2108 2847 2993 3056 3057 3218 3560 3638 3782 Size: 739 146 63 1 161 342 78 144 Cuts at: 3782 2108 Size: 5221 Fragments arranged by size: 5221 739 342 161 146 144 78 63 1 EcoRI G'AATT_C Cuts at: 2357 4204 4582 2357 Size: 1847 378 4670 Fragments arranged by size: 4670 1847 378 FspI TGC'GCA Cuts at: 5401 5401 Size: 6895 HaeII r_GCGC'y Cuts at: 1678 3684 3723 3810 6042 1678 Size: 2006 39 87 2232 2531 Fragments arranged by size: 2531 2232 2006 87 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3141 3173 3180 3202 3212 Size: 32 135 32 1569 32 7 22 10 Cuts at: 3212 3234 5536 5568 5610 5642 1373 Size: 22 2302 32 42 32 2626 Fragments arranged by size: 2626 2302 1569 135 42 32 32 32 32 32 22 22 10 7 HincII GTy'rAC Cuts at: 669 1657 1938 4239 4485 669 Size: 988 281 2301 246 3079 Fragments arranged by size: 3079 2301 988 281 246 HindIII A'AGCT_T Cuts at: 748 3901 748 Size: 3153 3742 HpaI GTT'AAC Cuts at: 4485 4485 Size: 6895 KasI G'GCGC_C Cuts at: 3680 3719 3680 Size: 39 6856 KpnI G_GTAC'C Cuts at: 4220 4220 Size: 6895 MfeI C'AATT_G Cuts at: 4494 4494 Size: 6895 MluI A'CGCG_T Cuts at: 6771 6771 Size: 6895 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2228 6009 6193 1305 Size: 923 3781 184 2007 Fragments arranged by size: 3781 2007 923 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2802 3659 3833 3950 10 Size: 54 1425 343 970 857 174 117 2955 Fragments arranged by size: 2955 1425 970 857 343 174 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 2978 3875 4058 4853 5212 5371 518 Size: 648 1812 897 183 795 359 159 2042 Fragments arranged by size: 2042 1812 897 795 648 359 183 159 MspA1I CmG'CkG Cuts at: 1606 2806 3382 4311 4988 6136 6381 1606 Size: 1200 576 929 677 1148 245 2120 Fragments arranged by size: 2120 1200 1148 929 677 576 245 NaeI GCC'GGC Cuts at: 3247 3469 3247 Size: 222 6673 NarI GG'CG_CC Cuts at: 3681 3720 3681 Size: 39 6856 NcoI C'CATG_G Cuts at: 513 1065 2004 2899 3429 3789 3834 513 Size: 552 939 895 530 360 45 3574 Fragments arranged by size: 3574 939 895 552 530 360 45 NdeI CA'TA_TG Cuts at: 387 2194 387 Size: 1807 5088 NgoMIV G'CCGG_C Cuts at: 3245 3467 3245 Size: 222 6673 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6895 NotI GC'GGCC_GC Cuts at: 4342 4342 Size: 6895 NsiI A_TGCA'T Cuts at: 2593 2593 Size: 6895 NspI r_CATG'y Cuts at: 1132 1229 2462 4253 5798 1132 Size: 97 1233 1791 1545 2229 Fragments arranged by size: 2229 1791 1545 1233 97 PasI CC'CwG_GG Cuts at: 1348 3200 3802 1348 Size: 1852 602 4441 Fragments arranged by size: 4441 1852 602 PciI A'CATG_T Cuts at: 5794 5794 Size: 6895 PflMI CCAn_nnn'nTGG Cuts at: 1108 3283 3430 1108 Size: 2175 147 4573 Fragments arranged by size: 4573 2175 147 PmeI GTTT'AAAC Cuts at: 4199 4199 Size: 6895 PmlI CAC'GTG Cuts at: 4039 4039 Size: 6895 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4964 4996 6477 6509 4964 Size: 32 1481 32 5350 Fragments arranged by size: 5350 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2993 3218 2993 Size: 225 6670 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6895 PsiI TTA'TAA Cuts at: 2643 4465 2643 Size: 1822 5073 PspOMI G'GGCC_C Cuts at: 2107 3056 3376 3559 3637 3781 3793 2107 Size: 949 320 183 78 144 12 5209 Fragments arranged by size: 5209 949 320 183 144 78 12 PspXI vC'TCGA_Gb Cuts at: 3731 3770 3731 Size: 39 6856 PstI C_TGCA'G Cuts at: 830 4247 830 Size: 3417 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6895 PvuII CAG'CTG Cuts at: 2806 2806 Size: 6895 SacI G_AGCT'C Cuts at: 2313 2313 Size: 6895 SalI G'TCGA_C Cuts at: 1936 4237 1936 Size: 2301 4594 SbfI CC_TGCA'GG Cuts at: 4247 4247 Size: 6895 ScaI AGT'ACT Cuts at: 5143 6855 5143 Size: 1712 5183 SexAI A'CCwGG_T Cuts at: 2271 2515 2271 Size: 244 6651 SfcI C'TryA_G Cuts at: 826 1045 4243 5378 6059 6250 826 Size: 219 3198 1135 681 191 1471 Fragments arranged by size: 3198 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3384 3956 3384 Size: 572 6323 SfoI GGC'GCC Cuts at: 3682 3721 3682 Size: 39 6856 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6895 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6895 SmaI CCC'GGG Cuts at: 1872 4222 1872 Size: 2350 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3485 3731 3770 4999 5900 6162 Size: 197 926 1542 246 39 1229 901 262 Cuts at: 6162 820 Size: 1553 Fragments arranged by size: 1553 1542 1229 926 901 262 246 197 39 SnaBI TAC'GTA Cuts at: 493 4686 493 Size: 4193 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6895 SphI G_CATG'C Cuts at: 1132 4253 1132 Size: 3121 3774 SspI AAT'ATT Cuts at: 5 52 4819 5 Size: 47 4767 2081 Fragments arranged by size: 4767 2081 47 StuI AGG'CCT Cuts at: 1517 3959 1517 Size: 2442 4453 StyI C'CwwG_G Cuts at: 513 1065 2004 2899 2905 3429 3789 3834 4077 Size: 552 939 895 6 524 360 45 243 Cuts at: 4077 4334 513 Size: 257 3074 Fragments arranged by size: 3074 939 895 552 524 360 257 243 45 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2560 3452 3746 3827 4922 4939 5092 Size: 1255 980 892 294 81 1095 17 153 Cuts at: 5092 5277 5722 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1095 980 892 445 294 185 153 81 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5141 6853 96 Size: 274 80 33 51 1443 3164 1712 138 Fragments arranged by size: 3164 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2535 2567 3386 3418 6855 6887 2535 Size: 32 819 32 3437 32 2543 Fragments arranged by size: 3437 2543 819 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6895 XbaI T'CTAG_A Cuts at: 4231 4231 Size: 6895 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2906 3511 3532 3574 2906 Size: 605 21 42 6227 Fragments arranged by size: 6227 605 42 21 XhoI C'TCGA_G Cuts at: 1943 3731 3770 1943 Size: 1788 39 5068 Fragments arranged by size: 5068 1788 39 XmaI C'CCGG_G Cuts at: 1870 4220 1870 Size: 2350 4545 XmnI GAAnn'nnTTC Cuts at: 5024 5024 Size: 6895 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4703 276 Size: 53 83 186 936 3169 2468 Fragments arranged by size: 3169 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI PacI PfoI PsrI RsrII SacII SanDI SapI SrfI SwaI