(Linear) (Six-base) MAPSORT of: KIEE2375.seq Check: 8797 from: 1 to: 2190 KIEE2375.orf With 182 enzymes: SgfI * October 20, 2011 13:24 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 341 679 1159 2190 Size: 341 338 480 1031 Fragments arranged by size: 1031 480 341 338 ApaI G_GGCC'C Cuts at: 0 106 1055 1375 1558 1636 1780 1792 2190 Size: 106 949 320 183 78 144 12 398 Fragments arranged by size: 949 398 320 183 144 106 78 12 ApoI r'AATT_y Cuts at: 0 352 2014 2190 Size: 352 1662 176 Fragments arranged by size: 1662 352 176 AscI GG'CGCG_CC Cuts at: 0 68 2190 Size: 68 2122 AvaI C'yCGr_G Cuts at: 0 1231 1726 1765 2190 Size: 1231 495 39 425 Fragments arranged by size: 1231 495 425 39 BanI G'GyrC_C Cuts at: 0 1237 1459 1596 1675 1714 1753 1846 2190 Size: 1237 222 137 79 39 39 93 344 Fragments arranged by size: 1237 344 222 137 93 79 39 39 BanII G_rGCy'C Cuts at: 0 99 106 308 1055 1294 1336 1354 1375 Size: 99 7 202 747 239 42 18 21 Cuts at: 1375 1537 1558 1636 1780 1792 2190 Size: 162 21 78 144 12 398 Fragments arranged by size: 747 398 239 202 162 144 99 78 42 21 21 18 12 7 BbeI G_GCGC'C Cuts at: 0 1679 1718 2190 Size: 1679 39 472 Fragments arranged by size: 1679 472 39 BbsI GAAGACnn'nnnn_ Cuts at: 0 423 606 2190 Size: 423 183 1584 Fragments arranged by size: 1584 423 183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1455 1489 2190 Size: 1455 34 701 Fragments arranged by size: 1455 701 34 BciVI GTATCCnnnnn_n' Cuts at: 0 556 2190 Size: 556 1634 BfrBI ATG'CAT Cuts at: 0 586 2190 Size: 586 1604 BglI GCCn_nnn'nGGC Cuts at: 0 1379 1951 2190 Size: 1379 572 239 Fragments arranged by size: 1379 572 239 Bme1580I G_kGCm'C Cuts at: 0 106 1055 1375 1558 1601 1636 1780 1792 Size: 106 949 320 183 43 35 144 12 Cuts at: 1792 2190 Size: 398 Fragments arranged by size: 949 398 320 183 144 106 43 35 12 BmgBI CAC'GTC Cuts at: 0 825 2190 Size: 825 1365 BmrI ACTGGGnnnn_n' Cuts at: 0 536 2190 Size: 536 1654 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1383 1642 1684 1708 1723 1762 1943 2190 Size: 1383 259 42 24 15 39 181 247 Fragments arranged by size: 1383 259 247 181 42 39 24 15 Bpu10I CC'TnA_GC Cuts at: 0 872 1579 2190 Size: 872 707 611 Fragments arranged by size: 872 707 611 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1501 2190 Size: 1501 689 BsaAI yAC'GTr Cuts at: 0 300 2034 2190 Size: 300 1734 156 Fragments arranged by size: 1734 300 156 BsaBI GATnn'nnATC Cuts at: 0 532 950 1089 2190 Size: 532 418 139 1101 Fragments arranged by size: 1101 532 418 139 BsaHI Gr'CG_yC Cuts at: 0 1676 1715 2190 Size: 1676 39 475 Fragments arranged by size: 1676 475 39 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1175 1205 1381 1411 2190 Size: 1175 30 176 30 779 Fragments arranged by size: 1175 779 176 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 182 1160 1184 2190 Size: 182 978 24 1006 Fragments arranged by size: 1006 978 182 24 BseYI C'CCAG_C Cuts at: 0 1377 2190 Size: 1377 813 BsiEI CG_ry'CG Cuts at: 0 1506 2190 Size: 1506 684 BsiHKAI G_wGCw'C Cuts at: 0 308 2190 Size: 308 1882 Bsp1286I G_dGCh'C Cuts at: 0 99 106 308 1055 1294 1336 1354 1375 Size: 99 7 202 747 239 42 18 21 Cuts at: 1375 1537 1558 1601 1636 1780 1792 2190 Size: 162 21 43 35 144 12 398 Fragments arranged by size: 747 398 239 202 162 144 99 43 42 35 21 21 18 12 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 135 1594 1894 2190 Size: 135 1459 300 296 Fragments arranged by size: 1459 300 296 135 BsrBI CCG'CTC Cuts at: 0 65 86 413 1495 1692 2190 Size: 65 21 327 1082 197 498 Fragments arranged by size: 1082 498 327 197 65 21 BsrDI GCAATG_nn' Cuts at: 0 473 1154 2190 Size: 473 681 1036 Fragments arranged by size: 1036 681 473 BsrFI r'CCGG_y Cuts at: 0 1240 1462 1657 2190 Size: 1240 222 195 533 Fragments arranged by size: 1240 533 222 195 BssHII G'CGCG_C Cuts at: 0 68 2190 Size: 68 2122 BstBI TT'CG_AA Cuts at: 0 2172 2190 Size: 2172 18 BstXI CCAn_nnnn'nTGG Cuts at: 0 901 1662 1686 1872 1936 2190 Size: 901 761 24 186 64 254 Fragments arranged by size: 901 761 254 186 64 24 BstYI r'GATC_y Cuts at: 0 438 1090 2190 Size: 438 652 1100 Fragments arranged by size: 1100 652 438 Bsu36I CC'TnA_GG Cuts at: 0 107 2190 Size: 107 2083 BtgI C'CryG_G Cuts at: 0 894 1272 1337 1424 1784 1829 2190 Size: 894 378 65 87 360 45 361 Fragments arranged by size: 894 378 361 360 87 65 45 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2061 2096 2190 Size: 2061 35 94 Fragments arranged by size: 2061 94 35 EaeI y'GGCC_r Cuts at: 0 795 1490 1652 1826 1943 2028 2111 2154 Size: 795 695 162 174 117 85 83 43 Cuts at: 2154 2190 Size: 36 Fragments arranged by size: 795 695 174 162 117 85 83 43 36 EarI CTCTTCn'nnn_ Cuts at: 0 53 136 359 1135 2190 Size: 53 83 223 776 1055 Fragments arranged by size: 1055 776 223 83 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 40 2190 Size: 40 2150 EcoICRI GAG'CTC Cuts at: 0 306 2190 Size: 306 1884 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 341 679 1159 1383 1642 1684 1708 1723 Size: 341 338 480 224 259 42 24 15 Cuts at: 1723 1762 1943 2190 Size: 39 181 247 Fragments arranged by size: 480 341 338 259 247 224 181 42 39 24 15 EcoO109I rG'GnC_Cy Cuts at: 0 103 842 988 1051 1052 1213 1555 1633 Size: 103 739 146 63 1 161 342 78 Cuts at: 1633 1777 2190 Size: 144 413 Fragments arranged by size: 739 413 342 161 146 144 103 78 63 1 EcoRI G'AATT_C Cuts at: 0 352 2190 Size: 352 1838 HaeII r_GCGC'y Cuts at: 0 1679 1718 1805 2190 Size: 1679 39 87 385 Fragments arranged by size: 1679 385 87 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1136 1168 1175 1197 1207 1229 2190 Size: 1136 32 7 22 10 22 961 Fragments arranged by size: 1136 961 32 22 22 10 7 HindIII A'AGCT_T Cuts at: 0 1896 2190 Size: 1896 294 KasI G'GCGC_C Cuts at: 0 1675 1714 2190 Size: 1675 39 476 Fragments arranged by size: 1675 476 39 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 223 2190 Size: 223 1967 MscI TGG'CCA Cuts at: 0 797 1654 1828 1945 2190 Size: 797 857 174 117 245 Fragments arranged by size: 857 797 245 174 117 MslI CAynn'nnrTG Cuts at: 0 973 1870 2053 2190 Size: 973 897 183 137 Fragments arranged by size: 973 897 183 137 MspA1I CmG'CkG Cuts at: 0 801 1377 2190 Size: 801 576 813 Fragments arranged by size: 813 801 576 NaeI GCC'GGC Cuts at: 0 1242 1464 2190 Size: 1242 222 726 Fragments arranged by size: 1242 726 222 NarI GG'CG_CC Cuts at: 0 1676 1715 2190 Size: 1676 39 475 Fragments arranged by size: 1676 475 39 NcoI C'CATG_G Cuts at: 0 894 1424 1784 1829 2190 Size: 894 530 360 45 361 Fragments arranged by size: 894 530 361 360 45 NdeI CA'TA_TG Cuts at: 0 189 2190 Size: 189 2001 NgoMIV G'CCGG_C Cuts at: 0 1240 1462 2190 Size: 1240 222 728 Fragments arranged by size: 1240 728 222 NsiI A_TGCA'T Cuts at: 0 588 2190 Size: 588 1602 NspI r_CATG'y Cuts at: 0 457 2190 Size: 457 1733 PasI CC'CwG_GG Cuts at: 0 1195 1797 2190 Size: 1195 602 393 Fragments arranged by size: 1195 602 393 PflMI CCAn_nnn'nTGG Cuts at: 0 1278 1425 2190 Size: 1278 147 765 Fragments arranged by size: 1278 765 147 PmlI CAC'GTG Cuts at: 0 2034 2190 Size: 2034 156 PpuMI rG'GwC_Cy Cuts at: 0 988 1213 2190 Size: 988 225 977 Fragments arranged by size: 988 977 225 PsiI TTA'TAA Cuts at: 0 638 2190 Size: 638 1552 PspOMI G'GGCC_C Cuts at: 0 102 1051 1371 1554 1632 1776 1788 2190 Size: 102 949 320 183 78 144 12 402 Fragments arranged by size: 949 402 320 183 144 102 78 12 PspXI vC'TCGA_Gb Cuts at: 0 1726 1765 2190 Size: 1726 39 425 Fragments arranged by size: 1726 425 39 PvuII CAG'CTG Cuts at: 0 801 2190 Size: 801 1389 SacI G_AGCT'C Cuts at: 0 308 2190 Size: 308 1882 SexAI A'CCwGG_T Cuts at: 0 266 510 2190 Size: 266 244 1680 Fragments arranged by size: 1680 266 244 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1379 1951 2190 Size: 1379 572 239 Fragments arranged by size: 1379 572 239 SfoI GGC'GCC Cuts at: 0 1677 1716 2190 Size: 1677 39 474 Fragments arranged by size: 1677 474 39 SmlI C'TyrA_G Cuts at: 0 1480 1726 1765 2190 Size: 1480 246 39 425 Fragments arranged by size: 1480 425 246 39 StuI AGG'CCT Cuts at: 0 1954 2190 Size: 1954 236 StyI C'CwwG_G Cuts at: 0 894 900 1424 1784 1829 2072 2190 Size: 894 6 524 360 45 243 118 Fragments arranged by size: 894 524 360 243 118 45 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 555 1447 1741 1822 2190 Size: 555 892 294 81 368 Fragments arranged by size: 892 555 368 294 81 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 530 562 1381 1413 2190 Size: 530 32 819 32 777 Fragments arranged by size: 819 777 530 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 901 1506 1527 1569 2190 Size: 901 605 21 42 621 Fragments arranged by size: 901 621 605 42 21 XhoI C'TCGA_G Cuts at: 0 1726 1765 2190 Size: 1726 39 425 Fragments arranged by size: 1726 425 39 Enzymes that do cut: AcuI ApaI ApoI AscI AvaI BanI BanII BbeI BbsI BcgI BciVI BfrBI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstBI BstXI BstYI Bsu36I BtgI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PflMI PmlI PpuMI PsiI PspOMI PspXI PvuII SacI SexAI SfiI SfoI SmlI StuI StyI TaqII TstI XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AseI AsiSI AvrII BaeI BamHI BbvCI BclI BglII BlpI BmtI BplI BsaI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstAPI BstEII BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI EagI EcoNI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI NheI NotI NruI PacI PciI PfoI PmeI PpiI PshAI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XmaI XmnI ZraI