################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI SacI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AlwNI 309 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG BanI 259 GGTGCC G^G[CT][GA]CC BglI 668 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 243 CTGCAC GTGCAG(16/14) BsmFI 231 GTCCC GGGAC(10/14) BsmI 684 GCATTC GAATGC(1/-1) BsrDI 534 CATTGC GCAATG(2/0) BstNI 489 CCAGG CC^[AT]GG BstXI 68 CCAAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 480 GCGC GCG^C CpoI 93 CGGACCG CG^G[AT]CCG DdeI 462 CTCAG C^T[ACGT]AG EarI 553 CTCTTC CTCTTC(1/4) EcoNI 708 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 489 CCAGG ^CC[AT]GG FauI 482 GCGGG CCCGC(4/6) HgaI 576 GACGC GACGC(5/10) HhaI 480 GCGC GCG^C HinP1I 480 GCGC G^CGC HinfI 704 GACTC G^A[ACGT]TC Ksp632I 553 CTCTTC CTCTTC(1/4) KspI 391 CCGCGG CCGC^GG MvaI 489 CCAGG CC^[AT]GG NaeI 134 GCCGGC GCC^GGC NgoMIV 134 GCCGGC G^CCGGC PflMI 102 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 704 GACTC GAGTC(4/5) PpuMI 380 AGGACCC [GA]G^G[AT]CC[CT] PshAI 576 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 93 CGGACCG CG^G[AT]CCG SacII 391 CCGCGG CCGC^GG SmaI 384 CCCGGG CCC^GGG TaqI 357 TCGA T^CGA TthHB8I 357 TCGA T^CGA Van91I 102 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 384 CCCGGG C^CCGGG XmaI 384 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 392 CGCG CG^CG 481 CGCG AfaI 158 GTAC GT^AC 609 GTAC AlwI 651 GATCC GGATC(4/5) 194 GGATC ApaI 485 GGGCCC GGGCC^C 675 GGGCCC AvaI 384 CCCGGG C^[CT]CG[GA]G 670 CCCGAG BanII 485 GGGCCC G[GA]GC[CT]^C 675 GGGCCC BmyI 485 GGGCCC G[AGT]GC[ACT]^C 675 GGGCCC BpmI 280 CTGGAG CTGGAG(16/14) 517 CTGGAG BsaI 529 GGTCTC GGTCTC(1/5) 582 GGTCTC BseRI 139 CTCCTC GAGGAG(10/8) 724 GAGGAG BsoBI 384 CCCGGG C^[CT]CG[GA]G 670 CCCGAG Bsp1286I 485 GGGCCC G[AGT]GC[ACT]^C 675 GGGCCC BsrFI 134 GCCGGC [GA]^CCGG[CT] 247 ACCGGC BstF5I 153 CATCC GGATG(2/0) 129 GGATG BstUI 392 CGCG CG^CG 481 CGCG BstYI 194 GGATCT [GA]^GATC[CT] 650 AGATCC Cfr10I 134 GCCGGC [GA]^CCGG[CT] 247 ACCGGC DpnII 195 GATC ^GATC 651 GATC DraIII 170 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 366 CACAGAGTG DsaI 391 CCGCGG C^C[GA][CT]GG 524 CCGTGG EaeI 132 TGGCCG [CT]^GGCC[GA] 452 TGGCCG EcoT14I 68 CCAAGG C^C[AT][AT]GG 161 CCTTGG FokI 153 CATCC GGATG(9/13) 129 GGATG MboI 195 GATC ^GATC 651 GATC MflI 194 GGATCT [GA]^GATC[CT] 650 AGATCC MvnI 392 CGCG CG^CG 481 CGCG NdeII 195 GATC ^GATC 651 GATC NlaIII 202 CATG CATG^ 423 CATG RsaI 158 GTAC GT^AC 609 GTAC Sau3AI 195 GATC ^GATC 651 GATC StyI 68 CCAAGG C^C[AT][AT]GG 161 CCTTGG XhoII 194 GGATCT [GA]^GATC[CT] 650 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI AvaII BbvI BcnI BsaJI BsiYI BslI BsmAI BsrI BssKI Cac8I Cfr13I DraII EcoO109I Fnu4HI HaeIII HapII HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MwoI NciI NlaIV PvuII Sau96I ScrFI TseI Tsp45I TspRI