Restriction map for
pFN21AE4035
(Circular) (Six-base) MAPSORT of: pFN21AE4035.seq Check: 5971 from: 1 to: 5443
pFN21AE4035 5443 bp
With 182 enzymes: SgfI *
March 28, 2012 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2402 2672 2735 3506 4890 5158 1632
Size: 770 270 63 771 1384 268 1917
Fragments arranged by size:
1917 1384 771 770 270 268 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5443
AlwNI CAG_nnn'CTG
Cuts at: 2319 3186 4758 2319
Size: 867 1572 3004
Fragments arranged by size:
3004 1572 867
ApaI G_GGCC'C
Cuts at: 2494 2684 2494
Size: 190 5253
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2752 2978 3130 5239 1493
Size: 1259 226 152 2109 1697
Fragments arranged by size:
2109 1697 1259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2389 2675 2768 1870
Size: 73 446 286 93 4545
Fragments arranged by size:
4545 446 286 93 73
BamHI G'GATC_C
Cuts at: 2773 3140 2773
Size: 367 5076
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2264 2764 4219 618
Size: 325 217 627 477 500 1455 1842
Fragments arranged by size:
1842 1455 627 500 477 325 217
BanII G_rGCy'C
Cuts at: 1391 2494 2684 2762 1391
Size: 1103 190 78 4072
Fragments arranged by size:
4072 1103 190 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3634 3668 1903
Size: 34 1697 34 3678
Fragments arranged by size:
3678 1697 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2679 4054 136
Size: 107 122 71 631 605 1007 1375 1525
Fragments arranged by size:
1525 1375 1007 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 2494 2684 3504 4660 1790
Size: 704 190 820 1156 2573
Fragments arranged by size:
2573 1156 820 704 190
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 3174 4132
Size: 416 53 83 399 24 684 1073 958
Cuts at: 4132 442
Size: 1753
Fragments arranged by size:
1753 1073 958 684 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2543 4103 1239
Size: 1067 237 1560 2579
Fragments arranged by size:
2579 1560 1067 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4433 4731 3568
Size: 865 298 4280
Fragments arranged by size:
4280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 2593 4106 882
Size: 617 1041 53 1513 2219
Fragments arranged by size:
2219 1513 1041 617 53
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3250 3632 4267 275
Size: 53 83 186 936 1717 382 635 1451
Fragments arranged by size:
1717 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3876 4274 4548 4695 5106 3876
Size: 398 274 147 411 4213
Fragments arranged by size:
4213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5218
Fragments arranged by size:
5218 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2744 1186
Size: 949 609 3885
Fragments arranged by size:
3885 949 609
BseYI C'CCAG_C
Cuts at: 2260 2685 4646 5142 2260
Size: 425 1961 496 2561
Fragments arranged by size:
2561 1961 496 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2233 1163
Size: 573 497 4373
Fragments arranged by size:
4373 573 497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3504 3589 4660 3504
Size: 85 1071 4287
Fragments arranged by size:
4287 1071 85
BsmI GAATG_Cn'
Cuts at: 2689 2954 3047 2689
Size: 265 93 5085
Fragments arranged by size:
5085 265 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2494 2684 2762 3504 3589 4660 1391
Size: 399 704 190 78 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 704 399 190 85 78
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 844
Size: 1954 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 1394
Size: 429 1507 3507
Fragments arranged by size:
3507 1507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2538 3938 4112 65
Size: 1183 1290 1400 174 1396
Fragments arranged by size:
1400 1396 1290 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2139 2252 4087 4274 1700
Size: 110 329 113 1835 187 2869
Fragments arranged by size:
2869 1835 329 187 113 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 2080
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2199 2655 2773 3140 3525 3542 4994 5080
Size: 227 456 118 367 385 17 1452 86
Cuts at: 5080 5092 5438 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 456 385 367 346 227 118
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2396 2529 513
Size: 552 1331 133 3427
Fragments arranged by size:
3427 1331 552 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2536 487
Size: 683 56 1310 3394
Fragments arranged by size:
3394 1310 683 56
BtsI GCAGTG_nn'
Cuts at: 2352 2602 2959 3753 3773 2352
Size: 250 357 794 20 4022
Fragments arranged by size:
4022 794 357 250 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DraIII CAC_nnn'GTG
Cuts at: 2180 2376 2180
Size: 196 5247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2457 2890
Size: 54 1425 50 275 18 307 320 433
Cuts at: 2890 3779 5408 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 433 320 307 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2564 3373 1353
Size: 1211 809 3423
Fragments arranged by size:
3423 1211 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2681 4030 4404 4550 387
Size: 1064 138 1092 1349 374 146 1280
Fragments arranged by size:
1349 1280 1092 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2402 2543 2672 2735 3506 4103
Size: 393 674 96 141 129 63 771 597
Cuts at: 4103 4890 5158 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 771 674 597 393 268 141
129 96 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2717 2717
Size: 5443
EcoO109I rG'GnC_Cy
Cuts at: 2386 2490 2680 2681 2386
Size: 104 190 1 5148
Fragments arranged by size:
5148 190 104 1
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 4590 1678
Size: 2912 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2170 2202 4084 4116 4158
Size: 32 135 32 598 32 1882 32 42
Cuts at: 4158 4190 1373
Size: 32 2626
Fragments arranged by size:
2626 1882 598 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3033 669
Size: 988 281 849 246 3079
Fragments arranged by size:
3079 988 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4557 4741 1305
Size: 3252 184 2007
Fragments arranged by size:
3252 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3621
Fragments arranged by size:
3621 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 3760 3919 518
Size: 648 2235 359 159 2042
Fragments arranged by size:
2235 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2398 2455 2521 2611 2705 2859 3536 4684
Size: 792 57 66 90 94 154 677 1148
Cuts at: 4684 4929 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 792 677 245 154 94 90
66 57
NaeI GCC'GGC
Cuts at: 2141 2141
Size: 5443
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4891
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NgoMIV G'CCGG_C
Cuts at: 2139 2139
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2801 4346 1132
Size: 97 1572 1545 2229
Fragments arranged by size:
2229 1572 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5443
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 1108
Size: 1005 4438
PfoI T'CCnGG_A
Cuts at: 2658 2658
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 2386
Size: 5443
PshAI GACnn'nnGTC
Cuts at: 1613 2585 1613
Size: 972 4471
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PspOMI G'GGCC_C
Cuts at: 2490 2680 2490
Size: 190 5253
PstI C_TGCA'G
Cuts at: 830 2795 830
Size: 1965 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
PvuII CAG'CTG
Cuts at: 2521 2611 2705 2521
Size: 90 94 5259
Fragments arranged by size:
5259 94 90
RsrII CG'GwC_CG
Cuts at: 2099 2099
Size: 5443
SacII CC_GC'GG
Cuts at: 2399 2399
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SfcI C'TryA_G
Cuts at: 826 1045 2791 3926 4607 4798 826
Size: 219 1746 1135 681 191 1471
Fragments arranged by size:
1746 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2391 2770 1872
Size: 519 379 4545
Fragments arranged by size:
4545 519 379
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3547 4448 4710 820
Size: 197 926 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2073 2166 2882 513
Size: 552 1008 93 716 3074
Fragments arranged by size:
3074 1008 716 552 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 3470 3487 3640 3825 4270 325
Size: 1255 672 1218 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1218 672 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2613 3689 5401
Size: 274 80 33 51 1443 636 1076 1712
Cuts at: 5401 96
Size: 138
Fragments arranged by size:
1712 1443 1076 636 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5403 5435 5403
Size: 32 5411
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2389 2768 1870
Size: 519 379 4545
Fragments arranged by size:
4545 519 379
XmnI GAAnn'nnTTC
Cuts at: 3572 3572
Size: 5443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI