(Linear) (Six-base) MAPSORT of: KIEE4035.seq Check: 2172 from: 1 to: 738 KIEE4035.orf With 182 enzymes: SgfI * March 22, 2012 17:13 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 667 730 738 Size: 397 270 63 8 Fragments arranged by size: 397 270 63 8 AlwNI CAG_nnn'CTG Cuts at: 0 314 738 Size: 314 424 ApaI G_GGCC'C Cuts at: 0 489 679 738 Size: 489 190 59 Fragments arranged by size: 489 190 59 AvaI C'yCGr_G Cuts at: 0 384 670 738 Size: 384 286 68 Fragments arranged by size: 384 286 68 BanI G'GyrC_C Cuts at: 0 259 738 Size: 259 479 BanII G_rGCy'C Cuts at: 0 489 679 738 Size: 489 190 59 Fragments arranged by size: 489 190 59 BglI GCCn_nnn'nGGC Cuts at: 0 674 738 Size: 674 64 Bme1580I G_kGCm'C Cuts at: 0 489 679 738 Size: 489 190 59 Fragments arranged by size: 489 190 59 BmrI ACTGGGnnnn_n' Cuts at: 0 96 738 Size: 96 642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 538 738 Size: 301 237 200 Fragments arranged by size: 301 237 200 BsaI GGTCTCn'nnnn_ Cuts at: 0 535 588 738 Size: 535 53 150 Fragments arranged by size: 535 150 53 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 130 738 Size: 130 608 BseYI C'CCAG_C Cuts at: 0 255 680 738 Size: 255 425 58 Fragments arranged by size: 425 255 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 228 738 Size: 228 510 BsmI GAATG_Cn' Cuts at: 0 684 738 Size: 684 54 Bsp1286I G_dGCh'C Cuts at: 0 489 679 738 Size: 489 190 59 Fragments arranged by size: 489 190 59 BsrDI GCAATG_nn' Cuts at: 0 533 738 Size: 533 205 BsrFI r'CCGG_y Cuts at: 0 134 247 738 Size: 134 113 491 Fragments arranged by size: 491 134 113 BstXI CCAn_nnnn'nTGG Cuts at: 0 75 738 Size: 75 663 BstYI r'GATC_y Cuts at: 0 194 650 738 Size: 194 456 88 Fragments arranged by size: 456 194 88 BtgI C'CryG_G Cuts at: 0 391 524 738 Size: 391 133 214 Fragments arranged by size: 391 214 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 531 738 Size: 531 207 BtsI GCAGTG_nn' Cuts at: 0 347 597 738 Size: 347 250 141 Fragments arranged by size: 347 250 141 DraIII CAC_nnn'GTG Cuts at: 0 175 371 738 Size: 175 196 367 Fragments arranged by size: 367 196 175 EaeI y'GGCC_r Cuts at: 0 132 452 738 Size: 132 320 286 Fragments arranged by size: 320 286 132 EarI CTCTTCn'nnn_ Cuts at: 0 559 738 Size: 559 179 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 676 738 Size: 676 62 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 301 397 538 667 730 738 Size: 301 96 141 129 63 8 Fragments arranged by size: 301 141 129 96 63 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 712 738 Size: 712 26 EcoO109I rG'GnC_Cy Cuts at: 0 381 485 675 676 738 Size: 381 104 190 1 62 Fragments arranged by size: 381 190 104 62 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 165 197 738 Size: 165 32 541 Fragments arranged by size: 541 165 32 MspA1I CmG'CkG Cuts at: 0 393 450 516 606 700 738 Size: 393 57 66 90 94 38 Fragments arranged by size: 393 94 90 66 57 38 NaeI GCC'GGC Cuts at: 0 136 738 Size: 136 602 NgoMIV G'CCGG_C Cuts at: 0 134 738 Size: 134 604 PflMI CCAn_nnn'nTGG Cuts at: 0 108 738 Size: 108 630 PfoI T'CCnGG_A Cuts at: 0 653 738 Size: 653 85 PpuMI rG'GwC_Cy Cuts at: 0 381 738 Size: 381 357 PshAI GACnn'nnGTC Cuts at: 0 580 738 Size: 580 158 PspOMI G'GGCC_C Cuts at: 0 485 675 738 Size: 485 190 63 Fragments arranged by size: 485 190 63 PvuII CAG'CTG Cuts at: 0 516 606 700 738 Size: 516 90 94 38 Fragments arranged by size: 516 94 90 38 RsrII CG'GwC_CG Cuts at: 0 94 738 Size: 94 644 SacII CC_GC'GG Cuts at: 0 394 738 Size: 394 344 SmaI CCC'GGG Cuts at: 0 386 738 Size: 386 352 StyI C'CwwG_G Cuts at: 0 68 161 738 Size: 68 93 577 Fragments arranged by size: 577 93 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 247 738 Size: 247 491 TatI w'GTAC_w Cuts at: 0 608 738 Size: 608 130 XmaI C'CCGG_G Cuts at: 0 384 738 Size: 384 354 Enzymes that do cut: AcuI AlwNI ApaI AvaI BanI BanII BglI Bme1580I BmrI BpmI BsaI BseRI BseYI BsgI BsmI Bsp1286I BsrDI BsrFI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I MspA1I NaeI NgoMIV PflMI PfoI PpuMI PshAI PspOMI PvuII RsrII SacII SmaI StyI TaqII TatI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI