(Circular) (Six-base) MAPSORT of: pFN21AE1715.seq Check: 9216 from: 1 to: 5359 pFN21AE1715 5359 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2403 2403 Size: 5359 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3169 278 Size: 53 83 186 936 1633 2468 Fragments arranged by size: 2468 1633 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2702 1937 Size: 765 4594 Acc65I G'GTAC_C Cuts at: 2680 2680 Size: 5359 AclI AA'CG_TT Cuts at: 1563 3486 3859 1563 Size: 1923 373 3063 Fragments arranged by size: 3063 1923 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 3422 4806 5074 1632 Size: 710 1080 1384 268 1917 Fragments arranged by size: 1917 1384 1080 710 268 AfeI AGC'GCT Cuts at: 2219 2315 2219 Size: 96 5263 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5162 AflIII A'CryG_T Cuts at: 1203 4258 5235 1203 Size: 3055 977 1327 Fragments arranged by size: 3055 1327 977 AgeI A'CCGG_T Cuts at: 4190 4190 Size: 5359 AhdI GACnn_n'nnGTC Cuts at: 4088 4088 Size: 5359 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5359 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5359 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2432 2464 2432 Size: 32 5327 AlwNI CAG_nnn'CTG Cuts at: 2656 3102 4674 2656 Size: 446 1572 3341 Fragments arranged by size: 3341 1572 446 ApaLI G'TGCA_C Cuts at: 3416 4572 3416 Size: 1156 4203 ApoI r'AATT_y Cuts at: 1493 2668 2894 3046 5155 1493 Size: 1175 226 152 2109 1697 Fragments arranged by size: 2109 1697 1175 226 152 AseI AT'TA_AT Cuts at: 160 1032 3913 160 Size: 872 2881 1606 Fragments arranged by size: 2881 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2575 2684 1870 Size: 73 70 562 109 4545 Fragments arranged by size: 4545 562 109 73 70 BamHI G'GATC_C Cuts at: 2689 3056 2689 Size: 367 4992 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2261 2680 4135 618 Size: 325 217 627 474 419 1455 1842 Fragments arranged by size: 1842 1455 627 474 419 325 217 BanII G_rGCy'C Cuts at: 1391 2678 1391 Size: 1287 4072 BbeI G_GCGC'C Cuts at: 2265 2265 Size: 5359 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4388 BbvCI CC'TCA_GC Cuts at: 2464 2464 Size: 5359 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3550 3584 1903 Size: 34 1613 34 3678 Fragments arranged by size: 3678 1613 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3251 4461 3251 Size: 1210 4149 BclI T'GATC_A Cuts at: 1548 1548 Size: 5359 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3970 136 Size: 107 122 71 631 605 2298 1525 Fragments arranged by size: 2298 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2193 2517 5354 2193 Size: 324 2837 2198 Fragments arranged by size: 2837 2198 324 BlpI GC'TnA_GC Cuts at: 2776 2776 Size: 5359 Bme1580I G_kGCm'C Cuts at: 1790 2409 3420 4576 1790 Size: 619 1011 1156 2573 Fragments arranged by size: 2573 1156 1011 619 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5359 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3090 4048 442 Size: 416 53 83 399 24 1673 958 1753 Fragments arranged by size: 1753 1673 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 4019 1239 Size: 899 1881 2579 Fragments arranged by size: 2579 1881 899 Bpu10I CC'TnA_GC Cuts at: 2464 2464 Size: 5359 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3484 4349 4647 3484 Size: 865 298 4196 Fragments arranged by size: 4196 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 4022 882 Size: 617 954 1569 2219 Fragments arranged by size: 2219 1569 954 617 BsaAI yAC'GTr Cuts at: 493 1204 3150 493 Size: 711 1946 2702 Fragments arranged by size: 2702 1946 711 BsaBI GATnn'nnATC Cuts at: 5353 5353 Size: 5359 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 3166 3548 4183 Size: 53 83 186 936 729 904 382 635 Cuts at: 4183 275 Size: 1451 Fragments arranged by size: 1451 936 904 729 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3792 4190 4464 4611 5022 3792 Size: 398 274 147 411 4129 Fragments arranged by size: 4129 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5134 Fragments arranged by size: 5134 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5359 BseYI C'CCAG_C Cuts at: 4562 5058 4562 Size: 496 4863 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2274 1163 Size: 573 538 4248 Fragments arranged by size: 4248 573 538 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2809 3570 4598 1540 Size: 275 186 808 761 1028 2301 Fragments arranged by size: 2301 1028 808 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2052 2132 3420 3505 4576 2052 Size: 80 1288 85 1071 2835 Fragments arranged by size: 2835 1288 1071 85 80 BsmI GAATG_Cn' Cuts at: 2495 2870 2963 2495 Size: 375 93 4891 Fragments arranged by size: 4891 375 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2132 2409 2678 3420 3505 4576 Size: 399 262 80 277 269 742 85 1071 Cuts at: 4576 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 277 269 262 85 80 BspEI T'CCGG_A Cuts at: 5022 5022 Size: 5359 BspHI T'CATG_A Cuts at: 3248 4978 3248 Size: 1730 3629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2091 2394 2403 2714 844 Size: 1247 303 9 311 3489 Fragments arranged by size: 3489 1247 311 303 9 BsrBI CCG'CTC Cuts at: 1394 1823 3246 1394 Size: 429 1423 3507 Fragments arranged by size: 3507 1423 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3854 4028 65 Size: 1183 2606 174 1396 Fragments arranged by size: 2606 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4003 4190 1700 Size: 110 2193 187 2869 Fragments arranged by size: 2869 2193 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5359 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5359 BssSI C'ACGA_G Cuts at: 3419 4431 3419 Size: 1012 4347 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5359 BstBI TT'CG_AA Cuts at: 4163 4163 Size: 5359 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5359 BstXI CCAn_nnnn'nTGG Cuts at: 2140 2140 Size: 5359 BstYI r'GATC_y Cuts at: 1972 2193 2517 2689 3056 3441 3458 4910 4996 Size: 221 324 172 367 385 17 1452 86 Cuts at: 4996 5008 5354 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 324 221 172 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2248 2613 513 Size: 552 939 244 365 3259 Fragments arranged by size: 3259 939 552 365 244 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2266 487 Size: 683 56 1040 3580 Fragments arranged by size: 3580 1040 683 56 BtsI GCAGTG_nn' Cuts at: 2262 2648 2875 3669 3689 2262 Size: 386 227 794 20 3932 Fragments arranged by size: 3932 794 386 227 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5359 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5324 DraI TTT'AAA Cuts at: 2663 2663 Size: 5359 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4366 809 Size: 507 3050 1802 Fragments arranged by size: 3050 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 2806 3695 Size: 54 1425 50 275 18 379 597 889 Cuts at: 3695 5324 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 597 379 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2806 1537 Size: 275 994 4090 Fragments arranged by size: 4090 994 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3289 1353 Size: 1936 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3946 4320 4466 387 Size: 1064 138 2357 374 146 1280 Fragments arranged by size: 2357 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2342 3422 4019 4806 5074 1239 Size: 393 506 204 1080 597 787 268 1524 Fragments arranged by size: 1524 1080 787 597 506 393 268 204 EcoO109I rG'GnC_Cy Cuts at: 2010 2010 Size: 5359 EcoRI G'AATT_C Cuts at: 2668 3046 2668 Size: 378 4981 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2461 Size: 5359 FspI TGC'GCA Cuts at: 3865 3865 Size: 5359 HaeII r_GCGC'y Cuts at: 1678 2221 2265 2317 4506 1678 Size: 543 44 52 2189 2531 Fragments arranged by size: 2531 2189 543 52 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4000 4032 4074 4106 1373 Size: 32 135 32 2428 32 42 32 2626 Fragments arranged by size: 2626 2428 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2703 2949 669 Size: 988 281 765 246 3079 Fragments arranged by size: 3079 988 765 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5359 HpaI GTT'AAC Cuts at: 2949 2949 Size: 5359 KasI G'GCGC_C Cuts at: 2261 2261 Size: 5359 KpnI G_GTAC'C Cuts at: 2684 2684 Size: 5359 MfeI C'AATT_G Cuts at: 2958 2958 Size: 5359 MluI A'CGCG_T Cuts at: 5235 5235 Size: 5359 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4473 4657 1305 Size: 3168 184 2007 Fragments arranged by size: 3168 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2211 10 Size: 54 1425 343 379 3158 Fragments arranged by size: 3158 1425 379 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 2165 2366 2600 3317 3676 3835 Size: 648 972 27 201 234 717 359 159 Cuts at: 3835 518 Size: 2042 Fragments arranged by size: 2042 972 717 648 359 234 201 159 27 MspA1I CmG'CkG Cuts at: 1606 2207 2775 3452 4600 4845 1606 Size: 601 568 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 601 568 245 NarI GG'CG_CC Cuts at: 2262 2262 Size: 5359 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3868 Fragments arranged by size: 3868 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5359 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5359 NotI GC'GGCC_GC Cuts at: 2806 2806 Size: 5359 NspI r_CATG'y Cuts at: 1132 1229 2717 4262 1132 Size: 97 1488 1545 2229 Fragments arranged by size: 2229 1545 1488 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5359 PciI A'CATG_T Cuts at: 4258 4258 Size: 5359 PflMI CCAn_nnn'nTGG Cuts at: 1108 2656 1108 Size: 1548 3811 PmeI GTTT'AAAC Cuts at: 2663 2663 Size: 5359 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3428 3460 4941 4973 3428 Size: 32 1481 32 3814 Fragments arranged by size: 3814 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2010 2010 Size: 5359 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5359 PsiI TTA'TAA Cuts at: 2929 2929 Size: 5359 PstI C_TGCA'G Cuts at: 830 2643 2711 830 Size: 1813 68 3478 Fragments arranged by size: 3478 1813 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5359 SalI G'TCGA_C Cuts at: 1936 2701 1936 Size: 765 4594 SanDI GG'GwC_CC Cuts at: 2010 2010 Size: 5359 SbfI CC_TGCA'GG Cuts at: 2711 2711 Size: 5359 ScaI AGT'ACT Cuts at: 3607 5319 3607 Size: 1712 3647 SexAI A'CCwGG_T Cuts at: 2655 2655 Size: 5359 SfcI C'TryA_G Cuts at: 826 1045 2639 2707 3842 4523 4714 826 Size: 219 1594 68 1135 681 191 1471 Fragments arranged by size: 1594 1471 1135 681 219 191 68 SfoI GGC'GCC Cuts at: 2263 2263 Size: 5359 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5359 SmaI CCC'GGG Cuts at: 1872 2015 2577 2686 1872 Size: 143 562 109 4545 Fragments arranged by size: 4545 562 143 109 SmlI C'TyrA_G Cuts at: 820 1017 1943 3463 4364 4626 820 Size: 197 926 1520 901 262 1553 Fragments arranged by size: 1553 1520 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3150 493 Size: 2657 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5359 SphI G_CATG'C Cuts at: 1132 2717 1132 Size: 1585 3774 SspI AAT'ATT Cuts at: 5 52 3283 5 Size: 47 3231 2081 Fragments arranged by size: 3231 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5359 StyI C'CwwG_G Cuts at: 513 1065 2004 2469 2798 513 Size: 552 939 465 329 3074 Fragments arranged by size: 3074 939 552 465 329 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3386 3403 3556 3741 4186 325 Size: 1255 1806 17 153 185 445 1498 Fragments arranged by size: 1806 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3605 5317 96 Size: 274 80 33 51 1443 1628 1712 138 Fragments arranged by size: 1712 1628 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2555 2587 5319 5351 2555 Size: 32 2732 32 2563 Fragments arranged by size: 2732 2563 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5359 XbaI T'CTAG_A Cuts at: 2695 2695 Size: 5359 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5359 XmaI C'CCGG_G Cuts at: 1870 2013 2575 2684 1870 Size: 143 562 109 4545 Fragments arranged by size: 4545 562 143 109 XmnI GAAnn'nnTTC Cuts at: 3488 3488 Size: 5359 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3167 276 Size: 53 83 186 936 1633 2468 Fragments arranged by size: 2468 1633 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SrfI SwaI XcmI