(Circular) (Six-base) MAPSORT of: pFN21AE4386.seq Check: 5494 from: 1 to: 5923 pFN21AE4386 5923 bp With 182 enzymes: SgfI * March 28, 2012 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2415 3071 2415 Size: 656 5267 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3986 5370 5638 1632 Size: 2354 1384 268 1917 Fragments arranged by size: 2354 1917 1384 268 AfeI AGC'GCT Cuts at: 2071 2071 Size: 5923 AflII C'TTAA_G Cuts at: 820 1017 3125 820 Size: 197 2108 3618 Fragments arranged by size: 3618 2108 197 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 2057 2883 1166 Size: 891 826 4206 Fragments arranged by size: 4206 891 826 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2934 1184 Size: 1750 4173 AlwNI CAG_nnn'CTG Cuts at: 2167 2407 2422 2575 3666 5238 2167 Size: 240 15 153 1091 1572 2852 Fragments arranged by size: 2852 1572 1091 240 153 15 ApaLI G'TGCA_C Cuts at: 2876 3980 5136 2876 Size: 1104 1156 3663 Fragments arranged by size: 3663 1156 1104 ApoI r'AATT_y Cuts at: 1493 2412 3232 3458 3610 5719 1493 Size: 919 820 226 152 2109 1697 Fragments arranged by size: 2109 1697 919 820 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2501 3248 1870 Size: 73 558 747 4545 Fragments arranged by size: 4545 747 558 73 BamHI G'GATC_C Cuts at: 2349 2752 3253 3620 2349 Size: 403 501 367 4652 Fragments arranged by size: 4652 501 403 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2217 2388 2691 2942 3059 Size: 325 217 627 430 171 303 251 117 Cuts at: 3059 3244 4699 618 Size: 185 1455 1842 Fragments arranged by size: 1842 1455 627 430 325 303 251 217 185 171 117 BanII G_rGCy'C Cuts at: 1391 3242 1391 Size: 1851 4072 BbeI G_GCGC'C Cuts at: 2221 2695 2946 2221 Size: 474 251 5198 Fragments arranged by size: 5198 474 251 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3152 928 Size: 971 1253 3699 Fragments arranged by size: 3699 1253 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2436 2700 2738 Size: 107 122 71 631 605 764 264 38 Cuts at: 2738 2940 4534 136 Size: 202 1594 1525 Fragments arranged by size: 1594 1525 764 631 605 264 202 122 107 71 38 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 2201 3340 2201 Size: 1139 4784 Bme1580I G_kGCm'C Cuts at: 1790 2880 3984 5140 1790 Size: 1090 1104 1156 2573 Fragments arranged by size: 2573 1156 1104 1090 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2851 3654 4612 Size: 416 53 83 399 24 1434 803 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1434 958 803 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 3235 2031 Size: 1204 4719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2414 2801 3063 4583 1239 Size: 992 183 387 262 1520 2579 Fragments arranged by size: 2579 1520 992 387 262 183 Bpu10I CC'TnA_GC Cuts at: 2073 2222 2073 Size: 149 5774 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2315 4048 4913 5211 2315 Size: 1733 865 298 3027 Fragments arranged by size: 3027 1733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2634 4586 882 Size: 617 1135 1952 2219 Fragments arranged by size: 2219 1952 1135 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2218 2692 2943 3730 Size: 53 83 186 936 685 474 251 787 Cuts at: 3730 4112 4747 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 787 685 635 474 382 251 186 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3083 3113 1178 Size: 30 165 30 1680 30 3988 Fragments arranged by size: 3988 1680 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5923 BseYI C'CCAG_C Cuts at: 2376 2511 2773 2938 2960 5126 5622 2376 Size: 135 262 165 22 2166 496 2677 Fragments arranged by size: 2677 2166 496 262 165 135 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 2531 2849 2904 1163 Size: 573 372 423 318 55 4182 Fragments arranged by size: 4182 573 423 372 318 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 3373 4134 5162 1540 Size: 275 186 24 1348 761 1028 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 24 BsiHKAI G_wGCw'C Cuts at: 2017 2880 3984 4069 5140 2017 Size: 863 1104 85 1071 2800 Fragments arranged by size: 2800 1104 1071 863 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2880 3242 3984 4069 5140 1391 Size: 399 227 863 362 742 85 1071 2174 Fragments arranged by size: 2174 1071 863 742 399 362 227 85 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2415 3071 3278 844 Size: 1571 656 207 3489 Fragments arranged by size: 3489 1571 656 207 BsrBI CCG'CTC Cuts at: 1394 1823 2010 2215 3099 3810 1394 Size: 429 187 205 884 711 3507 Fragments arranged by size: 3507 884 711 429 205 187 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2550 2718 2930 4567 4754 1700 Size: 110 740 168 212 1637 187 2869 Fragments arranged by size: 2869 1637 740 212 187 168 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 2205 1925 Size: 280 5643 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 2407 2554 794 Size: 1613 147 4163 Fragments arranged by size: 4163 1613 147 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2349 2752 3253 3620 4005 4022 5474 5560 Size: 377 403 501 367 385 17 1452 86 Cuts at: 5560 5572 5918 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 501 403 385 377 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2301 2622 2301 Size: 321 5602 BtgI C'CryG_G Cuts at: 513 1065 2758 2925 3201 513 Size: 552 1693 167 276 3235 Fragments arranged by size: 3235 1693 552 276 167 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2916 3170 3439 4233 4253 2916 Size: 254 269 794 20 4586 Fragments arranged by size: 4586 794 269 254 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DraIII CAC_nnn'GTG Cuts at: 2371 2371 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2303 4930 809 Size: 507 987 2627 1802 Fragments arranged by size: 2627 1802 987 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2158 2472 Size: 54 1425 50 275 18 192 136 314 Cuts at: 2472 2823 2928 2932 3085 3370 4259 5888 8 Size: 351 105 4 153 285 889 1629 43 Fragments arranged by size: 1629 1425 889 351 314 285 275 192 153 136 105 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2022 3370 1537 Size: 275 210 1348 4090 Fragments arranged by size: 4090 1348 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 2366 3853 1353 Size: 1013 1487 3423 Fragments arranged by size: 3423 1487 1013 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2094 2227 2622 4510 4884 5030 Size: 1064 138 505 133 395 1888 374 146 Cuts at: 5030 387 Size: 1280 Fragments arranged by size: 1888 1280 1064 505 395 374 146 138 133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2414 2801 3063 3986 4583 5370 Size: 393 599 183 387 262 923 597 787 Cuts at: 5370 5638 1239 Size: 268 1524 Fragments arranged by size: 1524 923 787 599 597 393 387 268 262 183 EcoO109I rG'GnC_Cy Cuts at: 2305 2305 Size: 5923 EcoRI G'AATT_C Cuts at: 2412 3232 3610 2412 Size: 820 378 4725 Fragments arranged by size: 4725 820 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3154 3154 Size: 5923 FseI GG_CCGG'CC Cuts at: 2934 2934 Size: 5923 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2073 2221 2695 2946 5070 1678 Size: 395 148 474 251 2124 2531 Fragments arranged by size: 2531 2124 474 395 251 148 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373 Size: 32 135 32 2992 32 42 32 2626 Fragments arranged by size: 2992 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2521 3267 3513 669 Size: 988 281 583 746 246 3079 Fragments arranged by size: 3079 988 746 583 281 246 HindIII A'AGCT_T Cuts at: 748 3122 748 Size: 2374 3549 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KasI G'GCGC_C Cuts at: 2217 2691 2942 2217 Size: 474 251 5198 Fragments arranged by size: 5198 474 251 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2042 3099 5037 5221 1305 Size: 737 1057 1938 184 2007 Fragments arranged by size: 2007 1938 1057 737 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2474 10 Size: 54 1425 343 642 3459 Fragments arranged by size: 3459 1425 642 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2883 3185 3881 4240 4399 518 Size: 648 891 826 302 696 359 159 2042 Fragments arranged by size: 2042 891 826 696 648 359 302 159 MspA1I CmG'CkG Cuts at: 1606 2200 2386 2833 2938 2964 3115 3339 4016 Size: 594 186 447 105 26 151 224 677 Cuts at: 4016 5164 5409 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 594 447 245 224 186 151 105 26 NaeI GCC'GGC Cuts at: 2932 2932 Size: 5923 NarI GG'CG_CC Cuts at: 2218 2692 2943 2218 Size: 474 251 5198 Fragments arranged by size: 5198 474 251 NcoI C'CATG_G Cuts at: 513 1065 3201 513 Size: 552 2136 3235 Fragments arranged by size: 3235 2136 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NgoMIV G'CCGG_C Cuts at: 2930 2930 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3281 4826 1132 Size: 97 2052 1545 2229 Fragments arranged by size: 2229 2052 1545 97 PasI CC'CwG_GG Cuts at: 1348 2559 2724 1348 Size: 1211 165 4547 Fragments arranged by size: 4547 1211 165 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2371 2470 3202 1108 Size: 1263 99 732 3829 Fragments arranged by size: 3829 1263 732 99 PfoI T'CCnGG_A Cuts at: 2442 2442 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 2305 Size: 5923 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PstI C_TGCA'G Cuts at: 830 2154 2322 2868 2913 3068 3177 3275 830 Size: 1324 168 546 45 155 109 98 3478 Fragments arranged by size: 3478 1324 546 168 155 109 98 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2200 2833 2938 2200 Size: 633 105 5185 Fragments arranged by size: 5185 633 105 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 3092 4171 5883 3092 Size: 1079 1712 3132 Fragments arranged by size: 3132 1712 1079 SfcI C'TryA_G Cuts at: 826 1045 2150 2318 2864 2902 2909 3064 3173 Size: 219 1105 168 546 38 7 155 109 Cuts at: 3173 3271 4406 5087 5278 826 Size: 98 1135 681 191 1471 Fragments arranged by size: 1471 1135 1105 681 546 219 191 168 155 109 98 38 7 SfoI GGC'GCC Cuts at: 2219 2693 2944 2219 Size: 474 251 5198 Fragments arranged by size: 5198 474 251 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2330 3125 4027 4928 5190 820 Size: 197 926 387 795 902 901 262 1553 Fragments arranged by size: 1553 926 902 901 795 387 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 3201 3362 513 Size: 552 2136 161 3074 Fragments arranged by size: 3074 2136 552 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2873 2974 3950 3967 4120 4305 4750 Size: 1255 1293 101 976 17 153 185 445 Cuts at: 4750 325 Size: 1498 Fragments arranged by size: 1498 1293 1255 976 445 185 153 101 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3090 4169 5881 Size: 274 80 33 51 1443 1113 1079 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1712 1443 1113 1079 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI