(Linear) (Six-base) MAPSORT of: KIEE4386.seq Check: 8991 from: 1 to: 1218 KIEE4386.orf With 182 enzymes: SgfI * March 22, 2012 17:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 410 1066 1218 Size: 410 656 152 Fragments arranged by size: 656 410 152 AfeI AGC'GCT Cuts at: 0 66 1218 Size: 66 1152 AflII C'TTAA_G Cuts at: 0 1120 1218 Size: 1120 98 AleI CACnn'nnGTG Cuts at: 0 52 878 1218 Size: 52 826 340 Fragments arranged by size: 826 340 52 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 929 1218 Size: 929 289 AlwNI CAG_nnn'CTG Cuts at: 0 162 402 417 570 1218 Size: 162 240 15 153 648 Fragments arranged by size: 648 240 162 153 15 ApaLI G'TGCA_C Cuts at: 0 871 1218 Size: 871 347 ApoI r'AATT_y Cuts at: 0 407 1218 Size: 407 811 AvaI C'yCGr_G Cuts at: 0 496 1218 Size: 496 722 BamHI G'GATC_C Cuts at: 0 344 747 1218 Size: 344 403 471 Fragments arranged by size: 471 403 344 BanI G'GyrC_C Cuts at: 0 212 383 686 937 1054 1218 Size: 212 171 303 251 117 164 Fragments arranged by size: 303 251 212 171 164 117 BbeI G_GCGC'C Cuts at: 0 216 690 941 1218 Size: 216 474 251 277 Fragments arranged by size: 474 277 251 216 BbsI GAAGACnn'nnnn_ Cuts at: 0 1147 1218 Size: 1147 71 BglI GCCn_nnn'nGGC Cuts at: 0 431 695 733 935 1218 Size: 431 264 38 202 283 Fragments arranged by size: 431 283 264 202 38 BlpI GC'TnA_GC Cuts at: 0 196 1218 Size: 196 1022 Bme1580I G_kGCm'C Cuts at: 0 875 1218 Size: 875 343 BmrI ACTGGGnnnn_n' Cuts at: 0 846 1218 Size: 846 372 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 26 1218 Size: 26 1192 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 409 796 1058 1218 Size: 226 183 387 262 160 Fragments arranged by size: 387 262 226 183 160 Bpu10I CC'TnA_GC Cuts at: 0 68 217 1218 Size: 68 149 1001 Fragments arranged by size: 1001 149 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 1218 Size: 310 908 BsaI GGTCTCn'nnnn_ Cuts at: 0 629 1218 Size: 629 589 BsaHI Gr'CG_yC Cuts at: 0 213 687 938 1218 Size: 213 474 251 280 Fragments arranged by size: 474 280 251 213 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1078 1108 1218 Size: 1078 30 110 Fragments arranged by size: 1078 110 30 BseYI C'CCAG_C Cuts at: 0 371 506 768 933 955 1218 Size: 371 135 262 165 22 263 Fragments arranged by size: 371 263 262 165 135 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 526 844 899 1218 Size: 103 423 318 55 319 Fragments arranged by size: 423 319 318 103 55 BsiEI CG_ry'CG Cuts at: 0 20 1218 Size: 20 1198 BsiHKAI G_wGCw'C Cuts at: 0 12 875 1218 Size: 12 863 343 Fragments arranged by size: 863 343 12 Bsp1286I G_dGCh'C Cuts at: 0 12 875 1218 Size: 12 863 343 Fragments arranged by size: 863 343 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 410 1066 1218 Size: 410 656 152 Fragments arranged by size: 656 410 152 BsrBI CCG'CTC Cuts at: 0 5 210 1094 1218 Size: 5 205 884 124 Fragments arranged by size: 884 205 124 5 BsrFI r'CCGG_y Cuts at: 0 545 713 925 1218 Size: 545 168 212 293 Fragments arranged by size: 545 293 212 168 BssHII G'CGCG_C Cuts at: 0 200 1218 Size: 200 1018 BstAPI GCAn_nnn'nTGC Cuts at: 0 402 549 1218 Size: 402 147 669 Fragments arranged by size: 669 402 147 BstYI r'GATC_y Cuts at: 0 344 747 1218 Size: 344 403 471 Fragments arranged by size: 471 403 344 Bsu36I CC'TnA_GG Cuts at: 0 296 617 1218 Size: 296 321 601 Fragments arranged by size: 601 321 296 BtgI C'CryG_G Cuts at: 0 753 920 1196 1218 Size: 753 167 276 22 Fragments arranged by size: 753 276 167 22 BtsI GCAGTG_nn' Cuts at: 0 911 1165 1218 Size: 911 254 53 Fragments arranged by size: 911 254 53 DraIII CAC_nnn'GTG Cuts at: 0 366 1218 Size: 366 852 DrdI GACnn_nn'nnGTC Cuts at: 0 298 1218 Size: 298 920 EaeI y'GGCC_r Cuts at: 0 17 153 467 818 923 927 1080 1218 Size: 17 136 314 351 105 4 153 138 Fragments arranged by size: 351 314 153 138 136 105 17 4 EagI C'GGCC_G Cuts at: 0 17 1218 Size: 17 1201 EarI CTCTTCn'nnn_ Cuts at: 0 361 1218 Size: 361 857 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 89 222 617 1218 Size: 89 133 395 601 Fragments arranged by size: 601 395 133 89 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 409 796 1058 1218 Size: 226 183 387 262 160 Fragments arranged by size: 387 262 226 183 160 EcoO109I rG'GnC_Cy Cuts at: 0 300 1218 Size: 300 918 EcoRI G'AATT_C Cuts at: 0 407 1218 Size: 407 811 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1149 1218 Size: 1149 69 FseI GG_CCGG'CC Cuts at: 0 929 1218 Size: 929 289 HaeII r_GCGC'y Cuts at: 0 68 216 690 941 1218 Size: 68 148 474 251 277 Fragments arranged by size: 474 277 251 148 68 HincII GTy'rAC Cuts at: 0 516 1218 Size: 516 702 HindIII A'AGCT_T Cuts at: 0 1117 1218 Size: 1117 101 KasI G'GCGC_C Cuts at: 0 212 686 937 1218 Size: 212 474 251 281 Fragments arranged by size: 474 281 251 212 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 37 1094 1218 Size: 37 1057 124 Fragments arranged by size: 1057 124 37 MscI TGG'CCA Cuts at: 0 469 1218 Size: 469 749 MslI CAynn'nnrTG Cuts at: 0 52 878 1180 1218 Size: 52 826 302 38 Fragments arranged by size: 826 302 52 38 MspA1I CmG'CkG Cuts at: 0 195 381 828 933 959 1110 1218 Size: 195 186 447 105 26 151 108 Fragments arranged by size: 447 195 186 151 108 105 26 NaeI GCC'GGC Cuts at: 0 927 1218 Size: 927 291 NarI GG'CG_CC Cuts at: 0 213 687 938 1218 Size: 213 474 251 280 Fragments arranged by size: 474 280 251 213 NcoI C'CATG_G Cuts at: 0 1196 1218 Size: 1196 22 NgoMIV G'CCGG_C Cuts at: 0 925 1218 Size: 925 293 PasI CC'CwG_GG Cuts at: 0 554 719 1218 Size: 554 165 499 Fragments arranged by size: 554 499 165 PflMI CCAn_nnn'nTGG Cuts at: 0 366 465 1197 1218 Size: 366 99 732 21 Fragments arranged by size: 732 366 99 21 PfoI T'CCnGG_A Cuts at: 0 437 1218 Size: 437 781 PpuMI rG'GwC_Cy Cuts at: 0 300 1218 Size: 300 918 PstI C_TGCA'G Cuts at: 0 149 317 863 908 1063 1172 1218 Size: 149 168 546 45 155 109 46 Fragments arranged by size: 546 168 155 149 109 46 45 PvuII CAG'CTG Cuts at: 0 195 828 933 1218 Size: 195 633 105 285 Fragments arranged by size: 633 285 195 105 ScaI AGT'ACT Cuts at: 0 1087 1218 Size: 1087 131 SfcI C'TryA_G Cuts at: 0 145 313 859 897 904 1059 1168 1218 Size: 145 168 546 38 7 155 109 50 Fragments arranged by size: 546 168 155 145 109 50 38 7 SfoI GGC'GCC Cuts at: 0 214 688 939 1218 Size: 214 474 251 279 Fragments arranged by size: 474 279 251 214 SmlI C'TyrA_G Cuts at: 0 325 1120 1218 Size: 325 795 98 Fragments arranged by size: 795 325 98 StyI C'CwwG_G Cuts at: 0 1196 1218 Size: 1196 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 868 969 1218 Size: 868 101 249 Fragments arranged by size: 868 249 101 TatI w'GTAC_w Cuts at: 0 1085 1218 Size: 1085 133 Enzymes that do cut: AarI AfeI AflII AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BbeI BbsI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI HaeII HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PpuMI PstI PvuII ScaI SfcI SfoI SmlI StyI TaqII TatI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BanII BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BsaAI BsaBI BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EcoICRI EcoNI EcoRV FspI FspAI Hin4I HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI