################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AvrII BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 102 CGCG CG^CG AcyI 334 GGCGTC G[GA]^CG[CT]C AfaI 281 GTAC GT^AC AlwI 513 GATCC GGATC(4/5) AviII 371 TGCGCA TGC^GCA BalI 797 TGGCCA TGG^CCA BbrPI 444 CACGTG CAC^GTG BbsI 831 GAAGAC GAAGAC(2/6) BcnI 519 CCCGG CC^[GC]GG BglI 395 GCCTAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 420 GCTGAGC GC^T[ACGT]AGC Bpu1102I 420 GCTGAGC GC^T[ACGT]AGC BpuAI 831 GAAGAC GAAGAC(2/6) BsaAI 444 CACGTG [CT]AC^GT[GA] BsaHI 334 GGCGTC G[GA]^CG[CT]C BsaI 659 GAGACC GGTCTC(1/5) BsmAI 659 GAGAC GTCTC(1/5) BspMI 21 GCAGGT ACCTGC(4/8) BsrBI 3 GAGCGG CCGCTC(-3/-3) BsrDI 294 GCAATG GCAATG(2/0) BsrFI 331 ACCGGC [GA]^CCGG[CT] BssSI 156 CTCGTG CACGAG(-5/-1) BstAPI 697 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 726 GGTCACC G^GT[ACGT]ACC BstPI 726 GGTCACC G^GT[ACGT]ACC BstUI 102 CGCG CG^CG BstYI 512 AGATCC [GA]^GATC[CT] CelII 420 GCTGAGC GC^T[ACGT]AGC Cfr10I 331 ACCGGC [GA]^CCGG[CT] DpnII 513 GATC ^GATC EcoNI 572 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 726 GGTCACC G^GT[ACGT]ACC FauI 108 CCCGC CCCGC(4/6) FspI 371 TGCGCA TGC^GCA HgaI 335 GCGTC GACGC(5/10) Hin1I 334 GGCGTC G[GA]^CG[CT]C MaeII 445 ACGT A^CGT MboI 513 GATC ^GATC MboII 831 GAAGA GAAGA(8/7) MflI 512 AGATCC [GA]^GATC[CT] MluNI 797 TGGCCA TGG^CCA MscI 797 TGGCCA TGG^CCA MspA1I 698 CAGCTG C[AC]G^C[GT]G MvnI 102 CGCG CG^CG NciI 519 CCCGG CC^[GC]GG NdeII 513 GATC ^GATC PmaCI 444 CACGTG CAC^GTG PmlI 444 CACGTG CAC^GTG PvuII 698 CAGCTG CAG^CTG RsaI 281 GTAC GT^AC Sau3AI 513 GATC ^GATC SfaNI 529 GATGC GCATC(5/9) SgrAI 330 CACCGGCG C[GA]^CCGG[CT]G StuI 87 AGGCCT AGG^CCT XhoII 512 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwNI 339 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 698 CAGCTGCTG ApaI 544 GGGCCC GGGCC^C 598 GGGCCC BfaI 85 CTAG C^TAG 397 CTAG BsgI 327 CTGCAC GTGCAG(16/14) 264 GTGCAG DsaI 539 CCATGG C^C[GA][CT]GG 593 CCATGG EaeI 459 CGGCCA [CT]^GGCC[GA] 797 TGGCCA EcoT14I 539 CCATGG C^C[AT][AT]GG 593 CCATGG HinfI 478 GACTC G^A[ACGT]TC 808 GAGTC MaeI 85 CTAG C^TAG 397 CTAG NcoI 539 CCATGG C^CATGG 593 CCATGG PleI 478 GACTC GAGTC(4/5) 808 GAGTC PstI 237 CTGCAG CTGCA^G 378 CTGCAG SacI 406 GAGCTC GAGCT^C 490 GAGCTC SfcI 237 CTGCAG C^T[GA][CT]AG 378 CTGCAG StyI 539 CCATGG C^C[AT][AT]GG 593 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AluI AspHI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MnlI MslI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI Tsp45I TspRI