Restriction map for
pFN21AE6686
(Linear) (Six-base) MAPSORT of: KIEE6686.seq Check: 326 from: 1 to: 846
KIEE6686.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AleI CACnn'nnGTG
Cuts at: 0 444 846
Size: 444 402
AlwNI CAG_nnn'CTG
Cuts at: 0 344 703 846
Size: 344 359 143
Fragments arranged by size:
359 344 143
ApaI G_GGCC'C
Cuts at: 0 548 602 846
Size: 548 54 244
Fragments arranged by size:
548 244 54
BanII G_rGCy'C
Cuts at: 0 48 109 410 494 548 602 765 846
Size: 48 61 301 84 54 54 163 81
Fragments arranged by size:
301 163 84 81 61 54 54 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 838 846
Size: 838 8
BglI GCCn_nnn'nGGC
Cuts at: 0 401 846
Size: 401 445
BlpI GC'TnA_GC
Cuts at: 0 421 846
Size: 421 425
Bme1580I G_kGCm'C
Cuts at: 0 163 206 548 602 846
Size: 163 43 342 54 244
Fragments arranged by size:
342 244 163 54 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 793 846
Size: 793 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 465 580 634 846
Size: 112 353 115 54 212
Fragments arranged by size:
353 212 115 112 54
Bpu10I CC'TnA_GC
Cuts at: 0 150 846
Size: 150 696
BsaI GGTCTCn'nnnn_
Cuts at: 0 653 846
Size: 653 193
BsaAI yAC'GTr
Cuts at: 0 446 846
Size: 446 400
BsaHI Gr'CG_yC
Cuts at: 0 335 846
Size: 335 511
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 274 639 730 846
Size: 271 3 365 91 116
Fragments arranged by size:
365 271 116 91 3
BseYI C'CCAG_C
Cuts at: 0 145 846
Size: 145 701
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 312 846
Size: 285 27 534
Fragments arranged by size:
534 285 27
BsiHKAI G_wGCw'C
Cuts at: 0 410 494 713 846
Size: 410 84 219 133
Fragments arranged by size:
410 219 133 84
BsmI GAATG_Cn'
Cuts at: 0 434 588 642 846
Size: 434 154 54 204
Fragments arranged by size:
434 204 154 54
Bsp1286I G_dGCh'C
Cuts at: 0 48 109 163 206 410 494 548 602
Size: 48 61 54 43 204 84 54 54
Cuts at: 602 713 765 846
Size: 111 52 81
Fragments arranged by size:
204 111 84 81 61 54 54 54
52 48 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 12 846
Size: 12 834
BsrBI CCG'CTC
Cuts at: 0 5 846
Size: 5 841
BsrDI GCAATG_nn'
Cuts at: 0 301 846
Size: 301 545
BsrFI r'CCGG_y
Cuts at: 0 331 846
Size: 331 515
BssSI C'ACGA_G
Cuts at: 0 156 846
Size: 156 690
BstAPI GCAn_nnn'nTGC
Cuts at: 0 703 846
Size: 703 143
BstEII G'GTnAC_C
Cuts at: 0 726 846
Size: 726 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 446 540 594 846
Size: 446 94 54 252
Fragments arranged by size:
446 252 94 54
BstYI r'GATC_y
Cuts at: 0 512 846
Size: 512 334
BtgI C'CryG_G
Cuts at: 0 539 593 846
Size: 539 54 253
Fragments arranged by size:
539 253 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 257 266 440 846
Size: 257 9 174 406
Fragments arranged by size:
406 257 174 9
EaeI y'GGCC_r
Cuts at: 0 459 797 846
Size: 459 338 49
Fragments arranged by size:
459 338 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 766 846
Size: 766 80
EcoICRI GAG'CTC
Cuts at: 0 408 492 846
Size: 408 84 354
Fragments arranged by size:
408 354 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 465 580 634 846
Size: 112 353 115 54 212
Fragments arranged by size:
353 212 115 112 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 576 846
Size: 576 270
EcoO109I rG'GnC_Cy
Cuts at: 0 142 544 545 598 599 846
Size: 142 402 1 53 1 247
Fragments arranged by size:
402 247 142 53 1 1
FspI TGC'GCA
Cuts at: 0 373 846
Size: 373 473
HaeII r_GCGC'y
Cuts at: 0 61 219 453 774 846
Size: 61 158 234 321 72
Fragments arranged by size:
321 234 158 72 61
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 846
Size: 6 840
MscI TGG'CCA
Cuts at: 0 799 846
Size: 799 47
MslI CAynn'nnrTG
Cuts at: 0 444 538 592 846
Size: 444 94 54 254
Fragments arranged by size:
444 254 94 54
MspA1I CmG'CkG
Cuts at: 0 700 846
Size: 700 146
NcoI C'CATG_G
Cuts at: 0 539 593 846
Size: 539 54 253
Fragments arranged by size:
539 253 54
PfoI T'CCnGG_A
Cuts at: 0 518 846
Size: 518 328
PmlI CAC'GTG
Cuts at: 0 446 846
Size: 446 400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 62 846
Size: 30 32 784
Fragments arranged by size:
784 32 30
PspOMI G'GGCC_C
Cuts at: 0 544 598 846
Size: 544 54 248
Fragments arranged by size:
544 248 54
PstI C_TGCA'G
Cuts at: 0 241 382 846
Size: 241 141 464
Fragments arranged by size:
464 241 141
PvuII CAG'CTG
Cuts at: 0 700 846
Size: 700 146
SacI G_AGCT'C
Cuts at: 0 410 494 846
Size: 410 84 352
Fragments arranged by size:
410 352 84
SfcI C'TryA_G
Cuts at: 0 237 378 846
Size: 237 141 468
Fragments arranged by size:
468 237 141
SgrAI Cr'CCGG_yG
Cuts at: 0 331 846
Size: 331 515
StuI AGG'CCT
Cuts at: 0 89 846
Size: 89 757
StyI C'CwwG_G
Cuts at: 0 539 593 846
Size: 539 54 253
Fragments arranged by size:
539 253 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 180 479 846
Size: 180 299 367
Fragments arranged by size:
367 299 180
Enzymes that do cut:
AleI AlwNI ApaI BanII BbsI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI EaeI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII MmeI
MscI MslI MspA1I NcoI PfoI PmlI PpiI PspOMI
PstI PvuII SacI SfcI SgrAI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BpuEI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI