################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MunI NarI NciI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 262 ACATGT A^C[GA][CT]GT AhdI 258 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 6 AGCT AG^CT AspEI 258 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 63 GTGCTC G[AT]GC[AT]^C AvaI 191 CCCGAG C^[CT]CG[GA]G BamHI 92 GGATCC G^GATCC BfaI 50 CTAG C^TAG BglI 188 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 63 GTGCTC G[AGT]GC[ACT]^C BsaI 256 GAGACC GGTCTC(1/5) BsaWI 295 ACCGGA [AT]^CCGG[AT] BsiHKAI 63 GTGCTC G[AT]GC[AT]^C BsmFI 173 GGGAC GGGAC(10/14) BsoBI 191 CCCGAG C^[CT]CG[GA]G Bsp1286I 63 GTGCTC G[AGT]GC[ACT]^C BspLU11I 262 ACATGT A^CATGT BssSI 237 CTCGTG CACGAG(-5/-1) BstF5I 243 GGATG GGATG(2/0) BstYI 92 GGATCC [GA]^GATC[CT] DpnII 93 GATC ^GATC DraII 173 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 68 CCATGG C^C[GA][CT]GG Eam1105I 258 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 173 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 68 CCATGG C^C[AT][AT]GG FauI 280 GCGGG CCCGC(4/6) FokI 243 GGATG GGATG(9/13) HaeIII 142 GGCC GG^CC HgaI 158 GCGTC GACGC(5/10) MaeI 50 CTAG C^TAG MboI 93 GATC ^GATC MboII 73 GAAGA GAAGA(8/7) MflI 92 GGATCC [GA]^GATC[CT] NaeI 106 GCCGGC GCC^GGC NcoI 68 CCATGG C^CATGG NdeII 93 GATC ^GATC NgoMIV 106 GCCGGC G^CCGGC NspI 262 ACATGT [GA]CATG^[CT] PpuMI 173 GGGACCT [GA]G^G[AT]CC[CT] Sau3AI 93 GATC ^GATC SspI 117 AATATT AAT^ATT StyI 68 CCATGG C^C[AT][AT]GG XcmI 167 CCAGGGGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 92 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 227 CCGC CCGC(-3/-1) 280 GCGG AlwI 93 GATCC GGATC(4/5) 92 GGATC AvaII 174 GGACC G^G[AT]CC 293 GGACC BpmI 165 CTCCAG CTGGAG(16/14) 145 CTGGAG BsgI 213 CTGCAC GTGCAG(16/14) 316 GTGCAG BsmAI 256 GAGAC GTCTC(1/5) 14 GTCTC BsrFI 106 GCCGGC [GA]^CCGG[CT] 208 ACCGGC BsrI 290 ACTGG ACTGG(1/-1) 273 CCAGT BssHII 249 GCGCGC G^CGCGC 301 GCGCGC Cfr10I 106 GCCGGC [GA]^CCGG[CT] 208 ACCGGC Cfr13I 174 GGACC G^G[ACGT]CC 293 GGACC HphI 161 TCACC GGTGA(8/7) 206 TCACC Sau96I 174 GGACC G^G[ACGT]CC 293 GGACC TspRI 268 CACTG CA[GC]TG[ACGT][ACGT]^ 274 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AccII BbvI BsaJI BseRI BsiYI BslI BssKI BstNI BstUI Cac8I CfoI EcoRII Fnu4HI HapII HhaI HinP1I HpaII ItaI MaeIII MnlI MspI MvaI MvnI MwoI NlaIII NlaIV ScrFI TseI Tsp45I