(Circular) (Six-base) MAPSORT of: pFN21AE5135.seq Check: 2447 from: 1 to: 5029 pFN21AE5135 5029 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2839 278 Size: 53 83 186 936 1303 2468 Fragments arranged by size: 2468 1303 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2372 1937 Size: 435 4594 Acc65I G'GTAC_C Cuts at: 2350 2350 Size: 5029 AclI AA'CG_TT Cuts at: 1563 3156 3529 1563 Size: 1593 373 3063 Fragments arranged by size: 3063 1593 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3092 4476 4744 1632 Size: 1460 1384 268 1917 Fragments arranged by size: 1917 1460 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4832 AflIII A'CryG_T Cuts at: 1203 2267 3928 4905 1203 Size: 1064 1661 977 1327 Fragments arranged by size: 1661 1327 1064 977 AgeI A'CCGG_T Cuts at: 3860 3860 Size: 5029 AhdI GACnn_n'nnGTC Cuts at: 2268 3758 2268 Size: 1490 3539 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5029 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5029 AlwNI CAG_nnn'CTG Cuts at: 2772 4344 2772 Size: 1572 3457 ApaLI G'TGCA_C Cuts at: 3086 4242 3086 Size: 1156 3873 ApoI r'AATT_y Cuts at: 1493 2338 2564 2716 4825 1493 Size: 845 226 152 2109 1697 Fragments arranged by size: 2109 1697 845 226 152 AseI AT'TA_AT Cuts at: 160 1032 3583 160 Size: 872 2551 1606 Fragments arranged by size: 2551 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5029 AvaI C'yCGr_G Cuts at: 1870 1943 2196 2354 1870 Size: 73 253 158 4545 Fragments arranged by size: 4545 253 158 73 BamHI G'GATC_C Cuts at: 2097 2359 2726 2097 Size: 262 367 4400 Fragments arranged by size: 4400 367 262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2350 3805 618 Size: 325 217 627 563 1455 1842 Fragments arranged by size: 1842 1455 627 563 325 217 BanII G_rGCy'C Cuts at: 1391 2348 1391 Size: 957 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4058 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3220 3254 1903 Size: 34 1283 34 3678 Fragments arranged by size: 3678 1283 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2921 4131 2921 Size: 1210 3819 BclI T'GATC_A Cuts at: 1548 1548 Size: 5029 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2199 3640 136 Size: 107 122 71 631 605 527 1441 1525 Fragments arranged by size: 1525 1441 631 605 527 122 107 71 BglII A'GATC_T Cuts at: 5024 5024 Size: 5029 BlpI GC'TnA_GC Cuts at: 2446 2446 Size: 5029 Bme1580I G_kGCm'C Cuts at: 1790 3090 4246 1790 Size: 1300 1156 2573 Fragments arranged by size: 2573 1300 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5029 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2760 3718 442 Size: 416 53 83 399 24 1343 958 1753 Fragments arranged by size: 1753 1343 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5029 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2155 2171 3689 1239 Size: 916 16 1518 2579 Fragments arranged by size: 2579 1518 916 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3154 4019 4317 3154 Size: 865 298 3866 Fragments arranged by size: 3866 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2255 3692 882 Size: 617 756 1437 2219 Fragments arranged by size: 2219 1437 756 617 BsaAI yAC'GTr Cuts at: 493 1204 2820 493 Size: 711 1616 2702 Fragments arranged by size: 2702 1616 711 BsaBI GATnn'nnATC Cuts at: 5023 5023 Size: 5029 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2836 3218 3853 275 Size: 53 83 186 936 1303 382 635 1451 Fragments arranged by size: 1451 1303 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2300 3462 3860 4134 4281 4692 2300 Size: 1162 398 274 147 411 2637 Fragments arranged by size: 2637 1162 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4804 Fragments arranged by size: 4804 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2015 2018 1186 Size: 826 3 3 4197 Fragments arranged by size: 4197 826 3 3 BseYI C'CCAG_C Cuts at: 4232 4728 4232 Size: 496 4533 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2203 2342 1163 Size: 573 467 139 3850 Fragments arranged by size: 3850 573 467 139 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2479 3240 4268 1540 Size: 275 186 478 761 1028 2301 Fragments arranged by size: 2301 1028 761 478 275 186 BsiHKAI G_wGCw'C Cuts at: 2072 3090 3175 4246 2072 Size: 1018 85 1071 2855 Fragments arranged by size: 2855 1071 1018 85 BsmI GAATG_Cn' Cuts at: 2540 2633 2540 Size: 93 4936 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2348 3090 3175 4246 1391 Size: 399 282 276 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 276 85 BspEI T'CCGG_A Cuts at: 4692 4692 Size: 5029 BspHI T'CATG_A Cuts at: 2918 4648 2918 Size: 1730 3299 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2384 844 Size: 1540 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2916 1394 Size: 429 1093 3507 Fragments arranged by size: 3507 1093 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3524 3698 65 Size: 1183 2276 174 1396 Fragments arranged by size: 2276 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 2213 3673 3860 1700 Size: 110 301 102 1460 187 2869 Fragments arranged by size: 2869 1460 301 187 110 102 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5029 BssHII G'CGCG_C Cuts at: 1925 2254 2306 1925 Size: 329 52 4648 Fragments arranged by size: 4648 329 52 BssSI C'ACGA_G Cuts at: 2242 3089 4101 2242 Size: 847 1012 3170 Fragments arranged by size: 3170 1012 847 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5029 BstBI TT'CG_AA Cuts at: 3833 3833 Size: 5029 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5029 BstYI r'GATC_y Cuts at: 1972 2097 2359 2726 3111 3128 4580 4666 4678 Size: 125 262 367 385 17 1452 86 12 Cuts at: 4678 5024 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 262 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2073 513 Size: 552 1008 3469 Fragments arranged by size: 3469 1008 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4290 Fragments arranged by size: 4290 683 56 BtsI GCAGTG_nn' Cuts at: 2272 2545 3339 3359 2272 Size: 273 794 20 3942 Fragments arranged by size: 3942 794 273 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5029 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4994 DraI TTT'AAA Cuts at: 2333 2333 Size: 5029 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4036 809 Size: 507 2720 1802 Fragments arranged by size: 2720 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2476 3365 4994 Size: 54 1425 50 275 18 646 889 1629 Cuts at: 4994 8 Size: 43 Fragments arranged by size: 1629 1425 889 646 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2476 1537 Size: 275 664 4090 Fragments arranged by size: 4090 664 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2959 1353 Size: 1606 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2221 3616 3990 4136 387 Size: 1064 138 632 1395 374 146 1280 Fragments arranged by size: 1395 1280 1064 632 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2155 2171 3092 3689 4476 4744 1239 Size: 393 523 16 921 597 787 268 1524 Fragments arranged by size: 1524 921 787 597 523 393 268 16 EcoO109I rG'GnC_Cy Cuts at: 2179 2179 Size: 5029 EcoRI G'AATT_C Cuts at: 2338 2716 2338 Size: 378 4651 FspI TGC'GCA Cuts at: 3535 3535 Size: 5029 HaeII r_GCGC'y Cuts at: 1678 4176 1678 Size: 2498 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2254 2286 3670 3702 3744 Size: 32 135 32 682 32 1384 32 42 Cuts at: 3744 3776 1373 Size: 32 2626 Fragments arranged by size: 2626 1384 682 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2373 2619 669 Size: 988 281 435 246 3079 Fragments arranged by size: 3079 988 435 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5029 HpaI GTT'AAC Cuts at: 2619 2619 Size: 5029 KpnI G_GTAC'C Cuts at: 2354 2354 Size: 5029 MfeI C'AATT_G Cuts at: 2628 2628 Size: 5029 MluI A'CGCG_T Cuts at: 4905 4905 Size: 5029 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4143 4327 1305 Size: 2838 184 2007 Fragments arranged by size: 2838 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3207 Fragments arranged by size: 3207 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2987 3346 3505 518 Size: 648 1821 359 159 2042 Fragments arranged by size: 2042 1821 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2445 3122 4270 4515 1606 Size: 839 677 1148 245 2120 Fragments arranged by size: 2120 1148 839 677 245 NaeI GCC'GGC Cuts at: 2113 2113 Size: 5029 NcoI C'CATG_G Cuts at: 513 1065 2073 513 Size: 552 1008 3469 Fragments arranged by size: 3469 1008 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5029 NgoMIV G'CCGG_C Cuts at: 2111 2111 Size: 5029 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5029 NotI GC'GGCC_GC Cuts at: 2476 2476 Size: 5029 NspI r_CATG'y Cuts at: 1132 1229 2271 2387 3932 1132 Size: 97 1042 116 1545 2229 Fragments arranged by size: 2229 1545 1042 116 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5029 PciI A'CATG_T Cuts at: 2267 3928 2267 Size: 1661 3368 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5029 PfoI T'CCnGG_A Cuts at: 2100 2100 Size: 5029 PmeI GTTT'AAAC Cuts at: 2333 2333 Size: 5029 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3098 3130 4611 4643 3098 Size: 32 1481 32 3484 Fragments arranged by size: 3484 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2179 2179 Size: 5029 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5029 PsiI TTA'TAA Cuts at: 2599 2599 Size: 5029 PstI C_TGCA'G Cuts at: 830 2381 830 Size: 1551 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5029 SalI G'TCGA_C Cuts at: 1936 2371 1936 Size: 435 4594 SbfI CC_TGCA'GG Cuts at: 2381 2381 Size: 5029 ScaI AGT'ACT Cuts at: 3277 4989 3277 Size: 1712 3317 SfcI C'TryA_G Cuts at: 826 1045 2377 3512 4193 4384 826 Size: 219 1332 1135 681 191 1471 Fragments arranged by size: 1471 1332 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5029 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5029 SmaI CCC'GGG Cuts at: 1872 2356 1872 Size: 484 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3133 4034 4296 820 Size: 197 926 1190 901 262 1553 Fragments arranged by size: 1553 1190 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2820 493 Size: 2327 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5029 SphI G_CATG'C Cuts at: 1132 2387 1132 Size: 1255 3774 SspI AAT'ATT Cuts at: 5 52 2124 2953 5 Size: 47 2072 829 2081 Fragments arranged by size: 2081 2072 829 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5029 StyI C'CwwG_G Cuts at: 513 1065 2073 2468 513 Size: 552 1008 395 3074 Fragments arranged by size: 3074 1008 552 395 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3056 3073 3226 3411 3856 325 Size: 1255 1476 17 153 185 445 1498 Fragments arranged by size: 1498 1476 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3275 4987 96 Size: 274 80 33 51 1443 1298 1712 138 Fragments arranged by size: 1712 1443 1298 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4989 5021 4989 Size: 32 4997 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5029 XbaI T'CTAG_A Cuts at: 2365 2365 Size: 5029 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2179 Size: 5029 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5029 XmaI C'CCGG_G Cuts at: 1870 2354 1870 Size: 484 4545 XmnI GAAnn'nnTTC Cuts at: 3158 3158 Size: 5029 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2837 276 Size: 53 83 186 936 1303 2468 Fragments arranged by size: 2468 1303 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI