(Linear) (Six-base) MAPSORT of: KIEE5135.seq Check: 5409 from: 1 to: 324 KIEE5135.orf With 182 enzymes: SgfI * January 29, 2014 11:47 .. AflIII A'CryG_T Cuts at: 0 262 324 Size: 262 62 AhdI GACnn_n'nnGTC Cuts at: 0 263 324 Size: 263 61 AvaI C'yCGr_G Cuts at: 0 191 324 Size: 191 133 BamHI G'GATC_C Cuts at: 0 92 324 Size: 92 232 BglI GCCn_nnn'nGGC Cuts at: 0 194 324 Size: 194 130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 150 166 324 Size: 150 16 158 Fragments arranged by size: 158 150 16 BsaI GGTCTCn'nnnn_ Cuts at: 0 250 324 Size: 250 74 BsaWI w'CCGG_w Cuts at: 0 295 324 Size: 295 29 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 10 13 324 Size: 7 3 3 311 Fragments arranged by size: 311 7 3 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 198 324 Size: 198 126 BsiHKAI G_wGCw'C Cuts at: 0 67 324 Size: 67 257 Bsp1286I G_dGCh'C Cuts at: 0 67 324 Size: 67 257 BsrFI r'CCGG_y Cuts at: 0 106 208 324 Size: 106 102 116 Fragments arranged by size: 116 106 102 BssHII G'CGCG_C Cuts at: 0 249 301 324 Size: 249 52 23 Fragments arranged by size: 249 52 23 BssSI C'ACGA_G Cuts at: 0 237 324 Size: 237 87 BstYI r'GATC_y Cuts at: 0 92 324 Size: 92 232 BtgI C'CryG_G Cuts at: 0 68 324 Size: 68 256 BtsI GCAGTG_nn' Cuts at: 0 267 324 Size: 267 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 216 324 Size: 216 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 150 166 324 Size: 150 16 158 Fragments arranged by size: 158 150 16 EcoO109I rG'GnC_Cy Cuts at: 0 174 324 Size: 174 150 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 249 281 324 Size: 249 32 43 Fragments arranged by size: 249 43 32 NaeI GCC'GGC Cuts at: 0 108 324 Size: 108 216 NcoI C'CATG_G Cuts at: 0 68 324 Size: 68 256 NgoMIV G'CCGG_C Cuts at: 0 106 324 Size: 106 218 NspI r_CATG'y Cuts at: 0 266 324 Size: 266 58 PciI A'CATG_T Cuts at: 0 262 324 Size: 262 62 PfoI T'CCnGG_A Cuts at: 0 95 324 Size: 95 229 PpuMI rG'GwC_Cy Cuts at: 0 174 324 Size: 174 150 SspI AAT'ATT Cuts at: 0 119 324 Size: 119 205 StyI C'CwwG_G Cuts at: 0 68 324 Size: 68 256 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 174 324 Size: 174 150 Enzymes that do cut: AflIII AhdI AvaI BamHI BglI BpmI BsaI BsaWI BseRI BsgI BsiHKAI Bsp1286I BsrFI BssHII BssSI BstYI BtgI BtsI EciI Eco57MI EcoO109I Hin4I NaeI NcoI NgoMIV NspI PciI PfoI PpuMI SspI StyI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI