(Linear) (Six-base) MAPSORT of: KIEE1678.seq Check: 1001 from: 1 to: 579 KIEE1678.orf With 182 enzymes: SgfI * July 15, 2010 18:44 .. AfeI AGC'GCT Cuts at: 0 118 579 Size: 118 461 AleI CACnn'nnGTG Cuts at: 0 159 579 Size: 159 420 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 439 579 Size: 439 140 AlwNI CAG_nnn'CTG Cuts at: 0 141 471 579 Size: 141 330 108 Fragments arranged by size: 330 141 108 ApaI G_GGCC'C Cuts at: 0 235 579 Size: 235 344 AvaI C'yCGr_G Cuts at: 0 148 485 579 Size: 148 337 94 Fragments arranged by size: 337 148 94 BanI G'GyrC_C Cuts at: 0 365 579 Size: 365 214 BanII G_rGCy'C Cuts at: 0 235 579 Size: 235 344 BbvCI CC'TCA_GC Cuts at: 0 200 579 Size: 200 379 BciVI GTATCCnnnnn_n' Cuts at: 0 301 579 Size: 301 278 BlpI GC'TnA_GC Cuts at: 0 131 173 579 Size: 131 42 406 Fragments arranged by size: 406 131 42 Bme1580I G_kGCm'C Cuts at: 0 235 579 Size: 235 344 BmrI ACTGGGnnnn_n' Cuts at: 0 250 524 579 Size: 250 274 55 Fragments arranged by size: 274 250 55 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 345 579 Size: 345 234 Bpu10I CC'TnA_GC Cuts at: 0 200 579 Size: 200 379 BsaI GGTCTCn'nnnn_ Cuts at: 0 541 579 Size: 541 38 BsaAI yAC'GTr Cuts at: 0 289 579 Size: 289 290 BsaHI Gr'CG_yC Cuts at: 0 475 579 Size: 475 104 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 22 336 579 Size: 22 314 243 Fragments arranged by size: 314 243 22 BseYI C'CCAG_C Cuts at: 0 234 464 579 Size: 234 230 115 Fragments arranged by size: 234 230 115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 486 579 Size: 486 93 BsmBI CGTCTCn'nnnn_ Cuts at: 0 436 579 Size: 436 143 Bsp1286I G_dGCh'C Cuts at: 0 235 579 Size: 235 344 BsrBI CCG'CTC Cuts at: 0 147 248 579 Size: 147 101 331 Fragments arranged by size: 331 147 101 BsrDI GCAATG_nn' Cuts at: 0 455 579 Size: 455 124 BssSI C'ACGA_G Cuts at: 0 100 579 Size: 100 479 BstAPI GCAn_nnn'nTGC Cuts at: 0 141 570 579 Size: 141 429 9 Fragments arranged by size: 429 141 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 301 381 579 Size: 301 80 198 Fragments arranged by size: 301 198 80 BtgI C'CryG_G Cuts at: 0 258 386 579 Size: 258 128 193 Fragments arranged by size: 258 193 128 BtsI GCAGTG_nn' Cuts at: 0 571 579 Size: 571 8 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 430 465 579 Size: 430 35 114 Fragments arranged by size: 430 114 35 EaeI y'GGCC_r Cuts at: 0 335 383 579 Size: 335 48 196 Fragments arranged by size: 335 196 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 345 579 Size: 345 234 EcoO109I rG'GnC_Cy Cuts at: 0 38 196 207 231 579 Size: 38 158 11 24 348 Fragments arranged by size: 348 158 38 24 11 HaeII r_GCGC'y Cuts at: 0 120 471 579 Size: 120 351 108 Fragments arranged by size: 351 120 108 MscI TGG'CCA Cuts at: 0 337 579 Size: 337 242 MslI CAynn'nnrTG Cuts at: 0 159 299 310 325 579 Size: 159 140 11 15 254 Fragments arranged by size: 254 159 140 15 11 MspA1I CmG'CkG Cuts at: 0 141 183 579 Size: 141 42 396 Fragments arranged by size: 396 141 42 NcoI C'CATG_G Cuts at: 0 258 579 Size: 258 321 PpuMI rG'GwC_Cy Cuts at: 0 196 207 579 Size: 196 11 372 Fragments arranged by size: 372 196 11 PspOMI G'GGCC_C Cuts at: 0 231 579 Size: 231 348 PspXI vC'TCGA_Gb Cuts at: 0 148 579 Size: 148 431 PstI C_TGCA'G Cuts at: 0 182 579 Size: 182 397 PvuII CAG'CTG Cuts at: 0 141 183 579 Size: 141 42 396 Fragments arranged by size: 396 141 42 SfcI C'TryA_G Cuts at: 0 178 579 Size: 178 401 SmlI C'TyrA_G Cuts at: 0 148 579 Size: 148 431 SnaBI TAC'GTA Cuts at: 0 289 579 Size: 289 290 StyI C'CwwG_G Cuts at: 0 258 444 579 Size: 258 186 135 Fragments arranged by size: 258 186 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 35 149 558 579 Size: 35 114 409 21 Fragments arranged by size: 409 114 35 21 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 384 579 Size: 384 195 XhoI C'TCGA_G Cuts at: 0 148 579 Size: 148 431 Enzymes that do cut: AfeI AleI AlfI AlwNI ApaI AvaI BanI BanII BbvCI BciVI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsmBI Bsp1286I BsrBI BsrDI BssSI BstAPI BstXI BtgI BtsI CspCI EaeI Eco57MI EcoO109I HaeII MscI MslI MspA1I NcoI PpuMI PspOMI PspXI PstI PvuII SfcI SmlI SnaBI StyI TaqII XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BclI BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrFI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XmaI XmnI ZraI