################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AvrII BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BlnI BsaAI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI HaeII HindIII HpaI KasI KpnI Ksp632I MaeI MamI MfeI MluI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 1279 GACGCC G[GA]^CG[CT]C AflIII 143 ACGTGT A^C[GA][CT]GT ApaI 845 GGGCCC GGGCC^C Asp700I 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 749 TGCGCA TGC^GCA BalI 276 TGGCCA TGG^CCA BamHI 1031 GGATCC G^GATCC BbsI 1047 GAAGAC GAAGAC(2/6) BglII 248 AGATCT A^GATCT BlpI 990 GCTGAGC GC^T[ACGT]AGC BpmI 130 CTGGAG CTGGAG(16/14) Bpu1102I 990 GCTGAGC GC^T[ACGT]AGC BpuAI 1047 GAAGAC GAAGAC(2/6) BsaHI 1279 GACGCC G[GA]^CG[CT]C BsaI 592 GAGACC GGTCTC(1/5) BsmAI 592 GAGAC GTCTC(1/5) BspHI 488 TCATGA T^CATGA BstEII 705 GGTCACC G^GT[ACGT]ACC BstPI 705 GGTCACC G^GT[ACGT]ACC CelII 990 GCTGAGC GC^T[ACGT]AGC DraIII 679 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 705 GGTCACC G^GT[ACGT]ACC FseI 24 GGCCGGCC GGCCGG^CC FspI 749 TGCGCA TGC^GCA Hin1I 1279 GACGCC G[GA]^CG[CT]C HincII 763 GTCAAC GT[CT]^[GA]AC HindII 763 GTCAAC GT[CT]^[GA]AC HinfI 220 GACTC G^A[ACGT]TC KspI 1074 CCGCGG CCGC^GG MluNI 276 TGGCCA TGG^CCA MscI 276 TGGCCA TGG^CCA NspI 959 GCATGT [GA]CATG^[CT] PleI 220 GACTC GAGTC(4/5) PpuMI 668 GGGACCT [GA]G^G[AT]CC[CT] PstI 19 CTGCAG CTGCA^G RcaI 488 TCATGA T^CATGA SacI 862 GAGCTC GAGCT^C SacII 1074 CCGCGG CCGC^GG SbfI 18 CCTGCAGG CCTGCA^GG SfcI 19 CTGCAG C^T[GA][CT]AG SmaI 726 CCCGGG CCC^GGG SmlI 643 CTCAAG C^T[CT][GA]AG Sse8387I 18 CCTGCAGG CCTGCA^GG StuI 1175 AGGCCT AGG^CCT Tsp509I 172 AATT ^AATT XmaCI 726 CCCGGG C^CCGGG XmaI 726 CCCGGG C^CCGGG XmnI 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 13 GTGCAC G^TGCAC 565 GTGCAC AlwNI 985 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 1123 CAGGAGCTG ApaLI 13 GTGCAC G^TGCAC 565 GTGCAC AvaI 726 CCCGGG C^[CT]CG[GA]G 859 CCCGAG BcgI 38 GCAACCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1013 GCAGCTACTTCG BglI 29 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 386 GCCTTCAGGGC BsgI 787 CTGCAC GTGCAG(16/14) 997 GTGCAG BsoBI 726 CCCGGG C^[CT]CG[GA]G 859 CCCGAG BsrBI 185 GAGCGG CCGCTC(-3/-3) 699 GAGCGG BsrFI 25 GCCGGC [GA]^CCGG[CT] 289 GCCGGC Cfr10I 25 GCCGGC [GA]^CCGG[CT] 289 GCCGGC DdeI 991 CTGAG C^T[ACGT]AG 1259 CTGAG DraII 668 GGGACCT [GA]G^G[ACGT]CC[CT] 845 GGGCCCT EcoO109I 668 GGGACCT [GA]G^G[ACGT]CC[CT] 845 GGGCCCT EcoT14I 108 CCATGG C^C[AT][AT]GG 215 CCATGG HgaI 340 GACGC GACGC(5/10) 1279 GACGC NaeI 25 GCCGGC GCC^GGC 289 GCCGGC NcoI 108 CCATGG C^CATGG 215 CCATGG NgoMIV 25 GCCGGC G^CCGGC 289 GCCGGC SexAI 680 ACCTGGT A^CC[AT]GGT 1250 ACCTGGT StyI 108 CCATGG C^C[AT][AT]GG 215 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AccIII AciI AfaI AluI AlwI AspHI AspI AvaII BanI BanII BbvI BcnI BmyI BsaJI BsaWI BseAI BseRI BsiEI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspEI BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DpnII DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MroI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PflMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoII