(Linear) (Six-base) MAPSORT of: KIEE1910.seq Check: 9557 from: 1 to: 1332 KIEE1910.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 26 721 1332 Size: 26 695 611 Fragments arranged by size: 695 611 26 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 373 483 1102 1332 Size: 373 110 619 230 Fragments arranged by size: 619 373 230 110 AflIII A'CryG_T Cuts at: 0 143 1332 Size: 143 1189 AleI CACnn'nnGTG Cuts at: 0 535 1332 Size: 535 797 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1285 1317 1332 Size: 1285 32 15 Fragments arranged by size: 1285 32 15 AlwNI CAG_nnn'CTG Cuts at: 0 990 1128 1332 Size: 990 138 204 Fragments arranged by size: 990 204 138 ApaI G_GGCC'C Cuts at: 0 849 1332 Size: 849 483 ApaLI G'TGCA_C Cuts at: 0 13 565 1332 Size: 13 552 767 Fragments arranged by size: 767 552 13 AvaI C'yCGr_G Cuts at: 0 726 859 1332 Size: 726 133 473 Fragments arranged by size: 726 473 133 BamHI G'GATC_C Cuts at: 0 1031 1332 Size: 1031 301 BanI G'GyrC_C Cuts at: 0 211 507 855 1332 Size: 211 296 348 477 Fragments arranged by size: 477 348 296 211 BanII G_rGCy'C Cuts at: 0 545 849 866 1332 Size: 545 304 17 466 Fragments arranged by size: 545 466 304 17 BbsI GAAGACnn'nnnn_ Cuts at: 0 1054 1332 Size: 1054 278 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 27 61 1002 1036 1332 Size: 27 34 941 34 296 Fragments arranged by size: 941 296 34 34 27 BglI GCCn_nnn'nGGC Cuts at: 0 35 392 1332 Size: 35 357 940 Fragments arranged by size: 940 357 35 BglII A'GATC_T Cuts at: 0 248 1332 Size: 248 1084 BlpI GC'TnA_GC Cuts at: 0 991 1332 Size: 991 341 Bme1580I G_kGCm'C Cuts at: 0 17 314 445 512 569 849 860 1332 Size: 17 297 131 67 57 280 11 472 Fragments arranged by size: 472 297 280 131 67 57 17 11 BmrI ACTGGGnnnn_n' Cuts at: 0 309 1332 Size: 309 1023 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 1332 Size: 151 1181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 628 1332 Size: 628 704 BsaI GGTCTCn'nnnn_ Cuts at: 0 586 1332 Size: 586 746 BsaHI Gr'CG_yC Cuts at: 0 1280 1332 Size: 1280 52 BsaWI w'CCGG_w Cuts at: 0 283 359 433 1332 Size: 283 76 74 899 Fragments arranged by size: 899 283 76 74 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1285 1315 1332 Size: 1285 30 17 Fragments arranged by size: 1285 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 484 1306 1321 1324 1332 Size: 484 822 15 3 8 Fragments arranged by size: 822 484 15 8 3 BseYI C'CCAG_C Cuts at: 0 414 869 1332 Size: 414 455 463 Fragments arranged by size: 463 455 414 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 772 1018 1332 Size: 772 246 314 Fragments arranged by size: 772 314 246 BsiEI CG_ry'CG Cuts at: 0 259 924 1055 1289 1332 Size: 259 665 131 234 43 Fragments arranged by size: 665 259 234 131 43 BsiHKAI G_wGCw'C Cuts at: 0 17 569 866 1332 Size: 17 552 297 466 Fragments arranged by size: 552 466 297 17 Bsp1286I G_dGCh'C Cuts at: 0 17 314 445 512 545 569 849 860 Size: 17 297 131 67 33 24 280 11 Cuts at: 860 866 1332 Size: 6 466 Fragments arranged by size: 466 297 280 131 67 33 24 17 11 6 BspEI T'CCGG_A Cuts at: 0 283 359 433 1332 Size: 283 76 74 899 Fragments arranged by size: 899 283 76 74 BspHI T'CATG_A Cuts at: 0 488 1332 Size: 488 844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 26 131 721 755 1332 Size: 26 105 590 34 577 Fragments arranged by size: 590 577 105 34 26 BsrBI CCG'CTC Cuts at: 0 187 701 1332 Size: 187 514 631 Fragments arranged by size: 631 514 187 BsrFI r'CCGG_y Cuts at: 0 25 289 1332 Size: 25 264 1043 Fragments arranged by size: 1043 264 25 BstEII G'GTnAC_C Cuts at: 0 705 1332 Size: 705 627 BstYI r'GATC_y Cuts at: 0 248 461 1031 1332 Size: 248 213 570 301 Fragments arranged by size: 570 301 248 213 BtgI C'CryG_G Cuts at: 0 108 215 918 935 1074 1283 1332 Size: 108 107 703 17 139 209 49 Fragments arranged by size: 703 209 139 108 107 49 17 DraIII CAC_nnn'GTG Cuts at: 0 684 1332 Size: 684 648 EaeI y'GGCC_r Cuts at: 0 27 256 276 689 758 921 938 1052 Size: 27 229 20 413 69 163 17 114 Cuts at: 1052 1088 1115 1157 1286 1332 Size: 36 27 42 129 46 Fragments arranged by size: 413 229 163 129 114 69 46 42 36 27 27 20 17 EagI C'GGCC_G Cuts at: 0 256 921 1052 1286 1332 Size: 256 665 131 234 46 Fragments arranged by size: 665 256 234 131 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1330 1332 Size: 1330 2 EcoICRI GAG'CTC Cuts at: 0 864 1332 Size: 864 468 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 373 483 1102 1332 Size: 151 222 110 619 230 Fragments arranged by size: 619 230 222 151 110 EcoO109I rG'GnC_Cy Cuts at: 0 669 846 1332 Size: 669 177 486 Fragments arranged by size: 669 486 177 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1197 1332 Size: 1197 135 FseI GG_CCGG'CC Cuts at: 0 29 1332 Size: 29 1303 FspI TGC'GCA Cuts at: 0 751 1332 Size: 751 581 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1240 1272 1332 Size: 1240 32 60 Fragments arranged by size: 1240 60 32 HincII GTy'rAC Cuts at: 0 765 1332 Size: 765 567 MscI TGG'CCA Cuts at: 0 278 1332 Size: 278 1054 MslI CAynn'nnrTG Cuts at: 0 535 964 1159 1332 Size: 535 429 195 173 Fragments arranged by size: 535 429 195 173 MspA1I CmG'CkG Cuts at: 0 402 573 735 1076 1332 Size: 402 171 162 341 256 Fragments arranged by size: 402 341 256 171 162 NaeI GCC'GGC Cuts at: 0 27 291 1332 Size: 27 264 1041 Fragments arranged by size: 1041 264 27 NcoI C'CATG_G Cuts at: 0 108 215 1332 Size: 108 107 1117 Fragments arranged by size: 1117 108 107 NgoMIV G'CCGG_C Cuts at: 0 25 289 1332 Size: 25 264 1043 Fragments arranged by size: 1043 264 25 NspI r_CATG'y Cuts at: 0 963 1332 Size: 963 369 PflMI CCAn_nnn'nTGG Cuts at: 0 59 414 576 1332 Size: 59 355 162 756 Fragments arranged by size: 756 355 162 59 PfoI T'CCnGG_A Cuts at: 0 347 1121 1332 Size: 347 774 211 Fragments arranged by size: 774 347 211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1204 1236 1285 1317 1332 Size: 1204 32 49 32 15 Fragments arranged by size: 1204 49 32 32 15 PpuMI rG'GwC_Cy Cuts at: 0 669 1332 Size: 669 663 PspOMI G'GGCC_C Cuts at: 0 845 1332 Size: 845 487 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1000 1032 1332 Size: 1000 32 300 Fragments arranged by size: 1000 300 32 PstI C_TGCA'G Cuts at: 0 23 1332 Size: 23 1309 PvuII CAG'CTG Cuts at: 0 402 573 735 1332 Size: 402 171 162 597 Fragments arranged by size: 597 402 171 162 SacI G_AGCT'C Cuts at: 0 866 1332 Size: 866 466 SacII CC_GC'GG Cuts at: 0 1077 1332 Size: 1077 255 SbfI CC_TGCA'GG Cuts at: 0 23 1332 Size: 23 1309 SexAI A'CCwGG_T Cuts at: 0 680 1250 1332 Size: 680 570 82 Fragments arranged by size: 680 570 82 SfcI C'TryA_G Cuts at: 0 19 1332 Size: 19 1313 SmaI CCC'GGG Cuts at: 0 728 1332 Size: 728 604 SmlI C'TyrA_G Cuts at: 0 643 1332 Size: 643 689 StuI AGG'CCT Cuts at: 0 1177 1332 Size: 1177 155 StyI C'CwwG_G Cuts at: 0 108 215 1332 Size: 108 107 1117 Fragments arranged by size: 1117 108 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 232 883 1332 Size: 232 651 449 Fragments arranged by size: 651 449 232 TatI w'GTAC_w Cuts at: 0 146 1332 Size: 146 1186 Tth111I GACn'n_nGTC Cuts at: 0 223 343 355 1332 Size: 223 120 12 977 Fragments arranged by size: 977 223 120 12 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 189 894 972 1332 Size: 189 705 78 360 Fragments arranged by size: 705 360 189 78 XmaI C'CCGG_G Cuts at: 0 726 1332 Size: 726 606 XmnI GAAnn'nnTTC Cuts at: 0 496 1332 Size: 496 836 Enzymes that do cut: AarI AcuI AflIII AleI AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI BcgI BglI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BstEII BstYI BtgI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoO109I FalI FseI FspI Hin4I HincII MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PpiI PpuMI PspOMI PsrI PstI PvuII SacI SacII SbfI SexAI SfcI SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BclI BfrBI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsiWI BsmI BsmBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EarI EcoNI EcoRI EcoRV FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PvuI RsrII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XhoI ZraI