################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AflII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I AspEI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI HincII HindII HindIII HpaI Ksp632I KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 659 ACGTGT A^C[GA][CT]GT Alw44I 322 GTGCAC G^TGCAC Aor51HI 389 AGCGCT AGC^GCT ApaI 822 GGGCCC GGGCC^C ApaLI 322 GTGCAC G^TGCAC AspHI 322 GTGCAC G[AT]GC[AT]^C AviII 1058 TGCGCA TGC^GCA AvrII 133 CCTAGG C^CTAGG BalI 31 TGGCCA TGG^CCA BbeI 38 GGCGCC GGCGC^C BbrPI 874 CACGTG CAC^GTG BfaI 134 CTAG C^TAG BlnI 133 CCTAGG C^CTAGG BsaAI 874 CACGTG [CT]AC^GT[GA] BsgI 14 GTGCAG GTGCAG(16/14) BsiEI 285 CGACCG CG[GA][CT]^CG BsiHKAI 322 GTGCAC G[AT]GC[AT]^C BsrBI 547 CCGCTC CCGCTC(-3/-3) BstAPI 1013 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 896 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eco47III 389 AGCGCT AGC^GCT EcoNI 684 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 334 GATATC GAT^ATC FspI 1058 TGCGCA TGC^GCA KasI 38 GGCGCC G^GCGCC MaeI 134 CTAG C^TAG MaeIII 354 GTCAC ^GT[ACGT]AC MluNI 31 TGGCCA TGG^CCA MscI 31 TGGCCA TGG^CCA NarI 38 GGCGCC GG^CGCC NcoI 34 CCATGG C^CATGG PflMI 429 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 874 CACGTG CAC^GTG PmlI 874 CACGTG CAC^GTG SfaNI 118 GATGC GCATC(5/9) SfoI 38 GGCGCC GGC^GCC SmlI 649 CTCAAG C^T[CT][GA]AG TaqI 740 TCGA T^CGA TfiI 1039 GAATC G^A[AT]TC Tsp45I 354 GTCAC ^GT[GC]AC TthHB8I 740 TCGA T^CGA Van91I 429 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 911 GGTACC G^GTACC 1046 GGTACC AcyI 38 GGCGCC G[GA]^CG[CT]C 862 GACGCC Asp718I 911 GGTACC G^GTACC 1046 GGTACC AspI 310 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 708 GACGCTGTC AvaI 110 CCCGAG C^[CT]CG[GA]G 372 CCCGAG BamHI 3 GGATCC G^GATCC 127 GGATCC BpmI 514 CTGGAG CTGGAG(16/14) 793 CTGGAG BsaHI 38 GGCGCC G[GA]^CG[CT]C 862 GACGCC BsoBI 110 CCCGAG C^[CT]CG[GA]G 372 CCCGAG BspMI 98 ACCTGC ACCTGC(4/8) 914 ACCTGC BstF5I 219 CATCC GGATG(2/0) 870 CATCC FokI 219 CATCC GGATG(9/13) 870 CATCC HaeII 38 GGCGCC [GA]GCGC^[CT] 389 AGCGCT Hin1I 38 GGCGCC G[GA]^CG[CT]C 862 GACGCC KpnI 911 GGTACC GGTAC^C 1046 GGTACC MaeII 659 ACGT A^CGT 875 ACGT MboII 89 TCTTC GAAGA(8/7) 494 TCTTC MslI 219 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 870 CATCCACGTG PflFI 310 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 708 GACGCTGTC PleI 484 GACTC GAGTC(4/5) 607 GACTC PpuMI 148 GGGTCCT [GA]G^G[AT]CC[CT] 899 AGGACCT PvuII 67 CAGCTG CAG^CTG 993 CAGCTG SbfI 636 CCTGCAGG CCTGCA^GG 915 CCTGCAGG Sse8387I 636 CCTGCAGG CCTGCA^GG 915 CCTGCAGG StuI 797 AGGCCT AGG^CCT 1016 AGGCCT Tth111I 310 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC 708 GACGCTGTC XcmI 25 CCACAATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 563 CCAAGACCGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BanII BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrI BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MboI MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI TspRI XhoII