(Circular) (Six-base) MAPSORT of: pFN21AE6892.seq Check: 4860 from: 1 to: 5788 pFN21AE6892 5788 bp With 182 enzymes: SgfI * April 28, 2014 11:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 2916 3051 3109 2916 Size: 135 58 5595 Fragments arranged by size: 5595 135 58 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2980 3851 5235 5503 1632 Size: 1348 871 1384 268 1917 Fragments arranged by size: 1917 1384 1348 871 268 AfeI AGC'GCT Cuts at: 2396 2396 Size: 5788 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 2664 4687 5664 1203 Size: 1461 2023 977 1327 Fragments arranged by size: 2023 1461 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 2077 2440 2641 2929 3531 5103 2077 Size: 363 201 288 602 1572 2762 Fragments arranged by size: 2762 1572 602 363 288 201 ApaI G_GGCC'C Cuts at: 2831 2831 Size: 5788 ApaLI G'TGCA_C Cuts at: 2327 3845 5001 2327 Size: 1518 1156 3114 Fragments arranged by size: 3114 1518 1156 ApoI r'AATT_y Cuts at: 1493 3097 3323 3475 5584 1493 Size: 1604 226 152 2109 1697 Fragments arranged by size: 2109 1697 1604 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2115 2377 3113 1870 Size: 73 172 262 736 4545 Fragments arranged by size: 4545 736 262 172 73 AvrII C'CTAG_G Cuts at: 2138 2138 Size: 5788 BamHI G'GATC_C Cuts at: 2008 2132 3118 3485 2008 Size: 124 986 367 4311 Fragments arranged by size: 4311 986 367 124 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2043 2257 2916 3051 3109 Size: 325 217 627 256 214 659 135 58 Cuts at: 3109 4564 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 659 627 325 256 217 214 135 58 BanII G_rGCy'C Cuts at: 1391 2146 2831 2900 3107 1391 Size: 755 685 69 207 4072 Fragments arranged by size: 4072 755 685 207 69 BbeI G_GCGC'C Cuts at: 2047 2047 Size: 5788 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2035 2389 2801 Size: 107 122 71 631 605 363 354 412 Cuts at: 2801 4399 136 Size: 1598 1525 Fragments arranged by size: 1598 1525 631 605 412 363 354 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 2262 2331 2817 2831 3007 3849 5005 1790 Size: 472 69 486 14 176 842 1156 2573 Fragments arranged by size: 2573 1156 842 486 472 176 69 14 BmgBI CAC'GTC Cuts at: 1318 2665 1318 Size: 1347 4441 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2142 2344 3519 Size: 416 53 83 399 24 725 202 1175 Cuts at: 3519 4477 442 Size: 958 1753 Fragments arranged by size: 1753 1175 958 725 416 399 202 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2540 2819 4448 1239 Size: 1301 279 1629 2579 Fragments arranged by size: 2579 1629 1301 279 Bpu10I CC'TnA_GC Cuts at: 2954 2954 Size: 5788 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2639 3913 4778 5076 2639 Size: 1274 865 298 3351 Fragments arranged by size: 3351 1274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4451 882 Size: 617 2952 2219 Fragments arranged by size: 2952 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2881 3579 493 Size: 711 1677 698 2702 Fragments arranged by size: 2702 1677 711 698 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2044 2868 3595 3977 Size: 53 83 186 936 511 824 727 382 Cuts at: 3977 4612 275 Size: 635 1451 Fragments arranged by size: 1451 936 824 727 635 511 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2513 2543 2660 2690 2702 Size: 30 165 30 1110 30 117 30 12 Cuts at: 2702 2732 1178 Size: 30 4234 Fragments arranged by size: 4234 1110 165 117 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5788 BseYI C'CCAG_C Cuts at: 2824 2831 3000 4991 5487 2824 Size: 7 169 1991 496 3125 Fragments arranged by size: 3125 1991 496 169 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2040 1163 Size: 573 304 4911 Fragments arranged by size: 4911 573 304 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2293 3238 3999 5027 1540 Size: 275 186 292 945 761 1028 2301 Fragments arranged by size: 2301 1028 945 761 292 275 186 BsiHKAI G_wGCw'C Cuts at: 2331 3849 3934 5005 2331 Size: 1518 85 1071 3114 Fragments arranged by size: 3114 1518 1071 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 BsmBI CGTCTCn'nnnn_ Cuts at: 2112 2705 2969 2112 Size: 593 264 4931 Fragments arranged by size: 4931 593 264 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2262 2331 2817 2831 2900 3007 Size: 399 356 116 69 486 14 69 107 Cuts at: 3007 3107 3849 3934 5005 1391 Size: 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 399 356 116 107 100 85 69 69 14 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 2928 3143 844 Size: 1268 816 215 3489 Fragments arranged by size: 3489 1268 816 215 BsrBI CCG'CTC Cuts at: 1394 1823 2554 3675 1394 Size: 429 731 1121 3507 Fragments arranged by size: 3507 1121 731 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4283 4457 65 Size: 1183 3035 174 1396 Fragments arranged by size: 3035 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 3024 794 Size: 2230 3558 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2908 2908 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2008 2132 2400 3118 3485 3870 3887 5339 Size: 36 124 268 718 367 385 17 1452 Cuts at: 5339 5425 5437 5783 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 718 385 367 346 268 124 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2039 2525 2817 513 Size: 552 939 35 486 292 3484 Fragments arranged by size: 3484 939 552 486 292 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 2060 2283 3304 4098 4118 2060 Size: 223 1021 794 20 3730 Fragments arranged by size: 3730 1021 794 223 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2036 2055 2533 Size: 54 1425 50 275 18 206 19 478 Cuts at: 2533 2565 3235 4124 5753 8 Size: 32 670 889 1629 43 Fragments arranged by size: 1629 1425 889 670 478 275 206 54 50 43 32 19 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3718 1353 Size: 2365 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2540 2819 2980 3851 4448 5235 5503 Size: 393 908 279 161 871 597 787 268 Cuts at: 5503 1239 Size: 1524 Fragments arranged by size: 1524 908 871 787 597 393 279 268 161 EcoNI CCTnn'n_nnAGG Cuts at: 2693 2693 Size: 5788 EcoO109I rG'GnC_Cy Cuts at: 2154 2828 2905 2154 Size: 674 77 5037 Fragments arranged by size: 5037 674 77 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 EcoRV GAT'ATC Cuts at: 2341 2341 Size: 5788 FspI TGC'GCA Cuts at: 3065 4294 3065 Size: 1229 4559 HaeII r_GCGC'y Cuts at: 1678 2047 2398 4935 1678 Size: 369 351 2537 2531 Fragments arranged by size: 2537 2531 369 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2330 2362 2513 2545 2702 Size: 32 135 32 758 32 151 32 157 Cuts at: 2702 2734 4429 4461 4503 4535 1373 Size: 32 1695 32 42 32 2626 Fragments arranged by size: 2626 1695 758 157 151 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KasI G'GCGC_C Cuts at: 2043 2043 Size: 5788 KpnI G_GTAC'C Cuts at: 2920 3055 3113 2920 Size: 135 58 5595 Fragments arranged by size: 5595 135 58 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2373 2517 2622 4902 5086 1305 Size: 1068 144 105 2280 184 2007 Fragments arranged by size: 2280 2007 1068 184 144 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 2038 10 Size: 54 1425 343 206 3760 Fragments arranged by size: 3760 1425 343 206 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 2879 3746 4105 4264 518 Size: 648 1062 651 867 359 159 2042 Fragments arranged by size: 2042 1062 867 651 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2049 2074 2244 3000 3204 3881 5029 5274 Size: 443 25 170 756 204 677 1148 245 Cuts at: 5274 1606 Size: 2120 Fragments arranged by size: 2120 1148 756 677 443 245 204 170 25 NarI GG'CG_CC Cuts at: 2044 2044 Size: 5788 NcoI C'CATG_G Cuts at: 513 1065 2004 2039 513 Size: 552 939 35 4262 Fragments arranged by size: 4262 939 552 35 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 2014 1348 Size: 666 5122 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 2440 1108 Size: 1332 4456 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PmlI CAC'GTG Cuts at: 2881 2881 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2154 2905 2154 Size: 751 5037 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2827 2827 Size: 5788 PstI C_TGCA'G Cuts at: 830 2646 2925 3039 3140 830 Size: 1816 279 114 101 3478 Fragments arranged by size: 3478 1816 279 114 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 2074 3000 2074 Size: 926 4862 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 2646 2925 3140 2646 Size: 279 215 5294 Fragments arranged by size: 5294 279 215 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2642 2921 3035 3136 4271 4952 5143 Size: 219 1597 279 114 101 1135 681 191 Cuts at: 5143 826 Size: 1471 Fragments arranged by size: 1597 1471 1135 681 279 219 191 114 101 SfoI GGC'GCC Cuts at: 2045 2045 Size: 5788 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 3115 1872 Size: 1243 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2654 3892 4793 5055 820 Size: 197 926 711 1238 901 262 1553 Fragments arranged by size: 1553 1238 926 901 711 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 2804 3023 1517 Size: 1287 219 4282 Fragments arranged by size: 4282 1287 219 StyI C'CwwG_G Cuts at: 513 1065 2004 2039 2138 2694 2901 3227 513 Size: 552 939 35 99 556 207 326 3074 Fragments arranged by size: 3074 939 556 552 326 207 99 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2006 2562 2588 3815 3832 3985 4170 Size: 1255 426 556 26 1227 17 153 185 Cuts at: 4170 4615 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1227 556 445 426 185 153 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4034 5746 96 Size: 274 80 33 51 1443 2057 1712 138 Fragments arranged by size: 2057 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5748 5780 5748 Size: 32 5756 Tth111I GACn'n_nGTC Cuts at: 1316 2318 2716 1316 Size: 1002 398 4388 Fragments arranged by size: 4388 1002 398 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2037 2575 2011 Size: 26 538 5224 Fragments arranged by size: 5224 538 26 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 3113 1870 Size: 1243 4545 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI