(Linear) (Six-base) MAPSORT of: KIEE6892.seq Check: 4623 from: 1 to: 1083 KIEE6892.orf With 182 enzymes: SgfI * April 28, 2014 10:22 .. Acc65I G'GTAC_C Cuts at: 0 911 1046 1083 Size: 911 135 37 Fragments arranged by size: 911 135 37 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 975 1083 Size: 975 108 AfeI AGC'GCT Cuts at: 0 391 1083 Size: 391 692 AflIII A'CryG_T Cuts at: 0 659 1083 Size: 659 424 AlwNI CAG_nnn'CTG Cuts at: 0 72 435 636 924 1083 Size: 72 363 201 288 159 Fragments arranged by size: 363 288 201 159 72 ApaI G_GGCC'C Cuts at: 0 826 1083 Size: 826 257 ApaLI G'TGCA_C Cuts at: 0 322 1083 Size: 322 761 AvaI C'yCGr_G Cuts at: 0 110 372 1083 Size: 110 262 711 Fragments arranged by size: 711 262 110 AvrII C'CTAG_G Cuts at: 0 133 1083 Size: 133 950 BamHI G'GATC_C Cuts at: 0 3 127 1083 Size: 3 124 956 Fragments arranged by size: 956 124 3 BanI G'GyrC_C Cuts at: 0 38 252 911 1046 1083 Size: 38 214 659 135 37 Fragments arranged by size: 659 214 135 38 37 BanII G_rGCy'C Cuts at: 0 141 826 895 1083 Size: 141 685 69 188 Fragments arranged by size: 685 188 141 69 BbeI G_GCGC'C Cuts at: 0 42 1083 Size: 42 1041 BglI GCCn_nnn'nGGC Cuts at: 0 30 384 796 1083 Size: 30 354 412 287 Fragments arranged by size: 412 354 287 30 Bme1580I G_kGCm'C Cuts at: 0 257 326 812 826 1002 1083 Size: 257 69 486 14 176 81 Fragments arranged by size: 486 257 176 81 69 14 BmgBI CAC'GTC Cuts at: 0 660 1083 Size: 660 423 BmrI ACTGGGnnnn_n' Cuts at: 0 137 339 1083 Size: 137 202 744 Fragments arranged by size: 744 202 137 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 814 1083 Size: 535 279 269 Fragments arranged by size: 535 279 269 Bpu10I CC'TnA_GC Cuts at: 0 949 1083 Size: 949 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 1083 Size: 634 449 BsaAI yAC'GTr Cuts at: 0 876 1083 Size: 876 207 BsaHI Gr'CG_yC Cuts at: 0 39 863 1083 Size: 39 824 220 Fragments arranged by size: 824 220 39 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 508 538 655 685 697 727 1083 Size: 508 30 117 30 12 30 356 Fragments arranged by size: 508 356 117 30 30 30 12 BseYI C'CCAG_C Cuts at: 0 819 826 995 1083 Size: 819 7 169 88 Fragments arranged by size: 819 169 88 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 1083 Size: 35 1048 BsiEI CG_ry'CG Cuts at: 0 288 1083 Size: 288 795 BsiHKAI G_wGCw'C Cuts at: 0 326 1083 Size: 326 757 BsmBI CGTCTCn'nnnn_ Cuts at: 0 107 700 964 1083 Size: 107 593 264 119 Fragments arranged by size: 593 264 119 107 Bsp1286I G_dGCh'C Cuts at: 0 141 257 326 812 826 895 1002 1083 Size: 141 116 69 486 14 69 107 81 Fragments arranged by size: 486 141 116 107 81 69 69 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 107 923 1083 Size: 107 816 160 Fragments arranged by size: 816 160 107 BsrBI CCG'CTC Cuts at: 0 549 1083 Size: 549 534 BstAPI GCAn_nnn'nTGC Cuts at: 0 1019 1083 Size: 1019 64 BstXI CCAn_nnnn'nTGG Cuts at: 0 903 1083 Size: 903 180 BstYI r'GATC_y Cuts at: 0 3 127 395 1083 Size: 3 124 268 688 Fragments arranged by size: 688 268 124 3 BtgI C'CryG_G Cuts at: 0 34 520 812 1083 Size: 34 486 292 271 Fragments arranged by size: 486 292 271 34 BtsI GCAGTG_nn' Cuts at: 0 55 278 1083 Size: 55 223 805 Fragments arranged by size: 805 223 55 EaeI y'GGCC_r Cuts at: 0 31 50 528 560 1083 Size: 31 19 478 32 523 Fragments arranged by size: 523 478 32 31 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 814 975 1083 Size: 535 279 161 108 Fragments arranged by size: 535 279 161 108 EcoNI CCTnn'n_nnAGG Cuts at: 0 688 1083 Size: 688 395 EcoO109I rG'GnC_Cy Cuts at: 0 149 823 900 1083 Size: 149 674 77 183 Fragments arranged by size: 674 183 149 77 EcoRV GAT'ATC Cuts at: 0 336 1083 Size: 336 747 FspI TGC'GCA Cuts at: 0 1060 1083 Size: 1060 23 HaeII r_GCGC'y Cuts at: 0 42 393 1083 Size: 42 351 690 Fragments arranged by size: 690 351 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 325 357 508 540 697 729 1083 Size: 325 32 151 32 157 32 354 Fragments arranged by size: 354 325 157 151 32 32 32 KasI G'GCGC_C Cuts at: 0 38 1083 Size: 38 1045 KpnI G_GTAC'C Cuts at: 0 915 1050 1083 Size: 915 135 33 Fragments arranged by size: 915 135 33 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 368 512 617 1083 Size: 368 144 105 466 Fragments arranged by size: 466 368 144 105 MscI TGG'CCA Cuts at: 0 33 1083 Size: 33 1050 MslI CAynn'nnrTG Cuts at: 0 223 874 1083 Size: 223 651 209 Fragments arranged by size: 651 223 209 MspA1I CmG'CkG Cuts at: 0 44 69 239 995 1083 Size: 44 25 170 756 88 Fragments arranged by size: 756 170 88 44 25 NarI GG'CG_CC Cuts at: 0 39 1083 Size: 39 1044 NcoI C'CATG_G Cuts at: 0 34 1083 Size: 34 1049 PasI CC'CwG_GG Cuts at: 0 9 1083 Size: 9 1074 PflMI CCAn_nnn'nTGG Cuts at: 0 435 1083 Size: 435 648 PmlI CAC'GTG Cuts at: 0 876 1083 Size: 876 207 PpuMI rG'GwC_Cy Cuts at: 0 149 900 1083 Size: 149 751 183 Fragments arranged by size: 751 183 149 PspOMI G'GGCC_C Cuts at: 0 822 1083 Size: 822 261 PstI C_TGCA'G Cuts at: 0 641 920 1034 1083 Size: 641 279 114 49 Fragments arranged by size: 641 279 114 49 PvuII CAG'CTG Cuts at: 0 69 995 1083 Size: 69 926 88 Fragments arranged by size: 926 88 69 SbfI CC_TGCA'GG Cuts at: 0 641 920 1083 Size: 641 279 163 Fragments arranged by size: 641 279 163 SfcI C'TryA_G Cuts at: 0 637 916 1030 1083 Size: 637 279 114 53 Fragments arranged by size: 637 279 114 53 SfoI GGC'GCC Cuts at: 0 40 1083 Size: 40 1043 SmlI C'TyrA_G Cuts at: 0 649 1083 Size: 649 434 StuI AGG'CCT Cuts at: 0 799 1018 1083 Size: 799 219 65 Fragments arranged by size: 799 219 65 StyI C'CwwG_G Cuts at: 0 34 133 689 896 1083 Size: 34 99 556 207 187 Fragments arranged by size: 556 207 187 99 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1 557 583 1083 Size: 1 556 26 500 Fragments arranged by size: 556 500 26 1 Tth111I GACn'n_nGTC Cuts at: 0 313 711 1083 Size: 313 398 372 Fragments arranged by size: 398 372 313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 32 570 1083 Size: 32 538 513 Fragments arranged by size: 538 513 32 Enzymes that do cut: Acc65I AcuI AfeI AflIII AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII BbeI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BstAPI BstXI BstYI BtgI BtsI EaeI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NcoI PasI PflMI PmlI PpuMI PspOMI PstI PvuII SbfI SfcI SfoI SmlI StuI StyI TaqII Tth111I XcmI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI BsaI BsaBI BsaWI BseRI BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI FalI FseI FspAI HincII HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI XmaI XmnI ZraI