################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AlwI AlwNI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DrdI DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MroI MscI MseI MunI NaeI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 418 GTCTAC GT^[AC][GT]AC AfaI 168 GTAC GT^AC AflIII 356 ACGCGT A^C[GA][CT]GT AhdI 514 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 5 AGCGCT AGC^GCT AspEI 514 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 427 CTCGGG C^[CT]CG[GA]G AviII 388 TGCGCA TGC^GCA BcgI 200 CGATGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 322 CTGGAG CTGGAG(16/14) BsgI 118 CTGCAC GTGCAG(16/14) BsmAI 367 GAGAC GTCTC(1/5) BsmBI 367 GAGACG CGTCTC(1/5) BsmFI 112 GTCCC GGGAC(10/14) BsoBI 427 CTCGGG C^[CT]CG[GA]G BspMI 102 ACCTGC ACCTGC(4/8) BsrBI 469 GAGCGG CCGCTC(-3/-3) BsrI 165 CCAGT ACTGG(1/-1) BssSI 559 CACGAG CACGAG(-5/-1) BstAPI 120 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 550 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 514 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 5 AGCGCT AGC^GCT EcoT14I 23 CCTTGG C^C[AT][AT]GG FspI 388 TGCGCA TGC^GCA MluI 356 ACGCGT A^CGCGT MslI 57 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 448 CAGCGG C[AC]G^C[GT]G RsaI 168 GTAC GT^AC SbfI 180 CCTGCAGG CCTGCA^GG SfaNI 196 GATGC GCATC(5/9) Sse8387I 180 CCTGCAGG CCTGCA^GG StyI 23 CCTTGG C^C[AT][AT]GG TspRI 32 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Alw44I 173 GTGCAC G^TGCAC 556 GTGCAC ApaI 188 GGGCCC GGGCC^C 262 GGGCCC ApaLI 173 GTGCAC G^TGCAC 556 GTGCAC BanI 394 GGCGCC G^G[CT][GA]CC 455 GGCGCC BanII 188 GGGCCC G[GA]GC[CT]^C 262 GGGCCC BbeI 394 GGCGCC GGCGC^C 455 GGCGCC BstF5I 62 GGATG GGATG(2/0) 195 GGATG DdeI 30 CTCAG C^T[ACGT]AG 85 CTCAG FokI 62 GGATG GGATG(9/13) 195 GGATG KasI 394 GGCGCC G^GCGCC 455 GGCGCC NarI 394 GGCGCC GG^CGCC 455 GGCGCC PstI 39 CTGCAG CTGCA^G 181 CTGCAG SfcI 39 CTGCAG C^T[GA][CT]AG 181 CTGCAG SfoI 394 GGCGCC GGC^GCC 455 GGCGCC TaqI 69 TCGA T^CGA 132 TCGA TthHB8I 69 TCGA T^CGA 132 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaII BbvI BcnI BglI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MnlI MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI