(Linear) (Six-base) MAPSORT of: KIEE5055.seq Check: 6027 from: 1 to: 591 KIEE5055.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. AccI GT'mk_AC Cuts at: 0 419 591 Size: 419 172 AfeI AGC'GCT Cuts at: 0 7 591 Size: 7 584 AflIII A'CryG_T Cuts at: 0 356 591 Size: 356 235 AhdI GACnn_n'nnGTC Cuts at: 0 519 591 Size: 519 72 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 64 414 591 Size: 64 350 177 Fragments arranged by size: 350 177 64 ApaI G_GGCC'C Cuts at: 0 192 266 591 Size: 192 74 325 Fragments arranged by size: 325 192 74 ApaLI G'TGCA_C Cuts at: 0 173 556 591 Size: 173 383 35 Fragments arranged by size: 383 173 35 AvaI C'yCGr_G Cuts at: 0 427 591 Size: 427 164 BanI G'GyrC_C Cuts at: 0 394 455 591 Size: 394 61 136 Fragments arranged by size: 394 136 61 BanII G_rGCy'C Cuts at: 0 192 266 591 Size: 192 74 325 Fragments arranged by size: 325 192 74 BbeI G_GCGC'C Cuts at: 0 398 459 591 Size: 398 61 132 Fragments arranged by size: 398 132 61 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 189 223 591 Size: 189 34 368 Fragments arranged by size: 368 189 34 BglI GCCn_nnn'nGGC Cuts at: 0 160 270 309 403 591 Size: 160 110 39 94 188 Fragments arranged by size: 188 160 110 94 39 Bme1580I G_kGCm'C Cuts at: 0 177 192 266 560 591 Size: 177 15 74 294 31 Fragments arranged by size: 294 177 74 31 15 BmrI ACTGGGnnnn_n' Cuts at: 0 159 591 Size: 159 432 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 591 Size: 343 248 BsaHI Gr'CG_yC Cuts at: 0 143 220 395 456 591 Size: 143 77 175 61 135 Fragments arranged by size: 175 143 135 77 61 BseYI C'CCAG_C Cuts at: 0 229 342 591 Size: 229 113 249 Fragments arranged by size: 249 229 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 591 Size: 103 488 BsiHKAI G_wGCw'C Cuts at: 0 38 177 560 591 Size: 38 139 383 31 Fragments arranged by size: 383 139 38 31 BsmBI CGTCTCn'nnnn_ Cuts at: 0 361 591 Size: 361 230 Bsp1286I G_dGCh'C Cuts at: 0 38 177 192 266 560 591 Size: 38 139 15 74 294 31 Fragments arranged by size: 294 139 74 38 31 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 111 591 Size: 111 480 BsrBI CCG'CTC Cuts at: 0 471 591 Size: 471 120 BssHII G'CGCG_C Cuts at: 0 276 278 412 477 479 571 591 Size: 276 2 134 65 2 92 20 Fragments arranged by size: 276 134 92 65 20 2 2 BssSI C'ACGA_G Cuts at: 0 559 591 Size: 559 32 BstAPI GCAn_nnn'nTGC Cuts at: 0 126 591 Size: 126 465 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 214 591 Size: 214 377 DraIII CAC_nnn'GTG Cuts at: 0 555 591 Size: 555 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 591 Size: 343 248 EcoO109I rG'GnC_Cy Cuts at: 0 75 188 189 262 263 591 Size: 75 113 1 73 1 328 Fragments arranged by size: 328 113 75 73 1 1 FspI TGC'GCA Cuts at: 0 390 591 Size: 390 201 HaeII r_GCGC'y Cuts at: 0 9 398 459 591 Size: 9 389 61 132 Fragments arranged by size: 389 132 61 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 505 537 591 Size: 505 32 54 Fragments arranged by size: 505 54 32 KasI G'GCGC_C Cuts at: 0 394 455 591 Size: 394 61 136 Fragments arranged by size: 394 136 61 MluI A'CGCG_T Cuts at: 0 356 591 Size: 356 235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 386 591 Size: 386 205 MslI CAynn'nnrTG Cuts at: 0 61 591 Size: 61 530 MspA1I CmG'CkG Cuts at: 0 450 591 Size: 450 141 NarI GG'CG_CC Cuts at: 0 395 456 591 Size: 395 61 135 Fragments arranged by size: 395 135 61 PasI CC'CwG_GG Cuts at: 0 224 591 Size: 224 367 PfoI T'CCnGG_A Cuts at: 0 527 591 Size: 527 64 PspOMI G'GGCC_C Cuts at: 0 188 262 591 Size: 188 74 329 Fragments arranged by size: 329 188 74 PstI C_TGCA'G Cuts at: 0 43 185 591 Size: 43 142 406 Fragments arranged by size: 406 142 43 SbfI CC_TGCA'GG Cuts at: 0 185 591 Size: 185 406 SfcI C'TryA_G Cuts at: 0 39 181 591 Size: 39 142 410 Fragments arranged by size: 410 142 39 SfoI GGC'GCC Cuts at: 0 396 457 591 Size: 396 61 134 Fragments arranged by size: 396 134 61 StyI C'CwwG_G Cuts at: 0 23 591 Size: 23 568 Enzymes that do cut: AccI AfeI AflIII AhdI AlfI ApaI ApaLI AvaI BanI BanII BbeI BcgI BglI Bme1580I BmrI BpmI BsaHI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BssHII BssSI BstAPI BtgZI DraIII Eco57MI EcoO109I FspI HaeII Hin4I KasI MluI MmeI MslI MspA1I NarI PasI PfoI PspOMI PstI SbfI SfcI SfoI StyI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AgeI AleI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI