################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII BamHI BbrPI BcgI BciVI BclI BfrI BglI BglII BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MboI MfeI MflI MluI MroI MseI MunI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcsI 418 GAATTT [GA]^AATT[CT] AflIII 184 ACGTGT A^C[GA][CT]GT ApaI 165 GGGCCC GGGCC^C ApoI 418 GAATTT [GA]^AATT[CT] AvrII 60 CCTAGG C^CTAGG BalI 494 TGGCCA TGG^CCA BanI 6 GGCGCC G^G[CT][GA]CC BbeI 6 GGCGCC GGCGC^C BbsI 253 GAAGAC GAAGAC(2/6) BbvCI 75 GCTGAGG CCTCAGC(-5/-2) BbvI 54 GCTGC GCAGC(8/12) BfaI 61 CTAG C^TAG BlnI 60 CCTAGG C^CTAGG BlpI 469 GCTGAGC GC^T[ACGT]AGC BpmI 282 CTGGAG CTGGAG(16/14) Bpu1102I 469 GCTGAGC GC^T[ACGT]AGC BpuAI 253 GAAGAC GAAGAC(2/6) BsaAI 183 TACGTG [CT]AC^GT[GA] BsgI 273 CTGCAC GTGCAG(16/14) BspMI 271 ACCTGC ACCTGC(4/8) BsrDI 225 GCAATG GCAATG(2/0) BsrFI 81 GCCGGC [GA]^CCGG[CT] BstNI 291 CCAGG CC^[AT]GG BstXI 104 CCACGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 469 GCTGAGC GC^T[ACGT]AGC Cfr10I 81 GCCGGC [GA]^CCGG[CT] DraII 286 AGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 494 TGGCCA [CT]^GGCC[GA] EcoO109I 286 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRII 291 CCAGG ^CC[AT]GG FauI 336 GCGGG CCCGC(4/6) HinfI 237 GAGTC G^A[ACGT]TC KasI 6 GGCGCC G^GCGCC MaeI 61 CTAG C^TAG MaeIII 427 GTGAC ^GT[ACGT]AC MboII 253 GAAGA GAAGA(8/7) MluNI 494 TGGCCA TGG^CCA MscI 494 TGGCCA TGG^CCA MslI 105 CACGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 12 CAGCGG C[AC]G^C[GT]G MvaI 291 CCAGG CC^[AT]GG NaeI 81 GCCGGC GCC^GGC NarI 6 GGCGCC GG^CGCC NcoI 359 CCATGG C^CATGG NgoMIV 81 GCCGGC G^CCGGC NlaIII 360 CATG CATG^ PflMI 156 CCATAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 237 GAGTC GAGTC(4/5) SfaNI 502 GCATC GCATC(5/9) SfoI 6 GGCGCC GGC^GCC SmlI 206 CTCAAG C^T[CT][GA]AG TseI 54 GCTGC G^C[AT]GC Tsp45I 427 GTGAC ^GT[GC]AC Tsp509I 419 AATT ^AATT Van91I 156 CCATAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 431 CCAAAACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 309 GTAC GT^AC 354 GTAC AvaI 168 CCCGGG C^[CT]CG[GA]G 454 CCCGGG AvaII 215 GGACC G^G[AT]CC 245 GGACC BanII 100 GAGCCC G[GA]GC[CT]^C 165 GGGCCC BmyI 100 GAGCCC G[AGT]GC[ACT]^C 165 GGGCCC BsmI 144 GCATTC GAATGC(1/-1) 460 GCATTC BsoBI 168 CCCGGG C^[CT]CG[GA]G 454 CCCGGG Bsp1286I 100 GAGCCC G[AGT]GC[ACT]^C 165 GGGCCC Cac8I 37 GCGAGC GC[ACGT]^[ACGT]GC 81 GCCGGC DsaI 68 CCGTGG C^C[GA][CT]GG 359 CCATGG HgaI 29 GCGTC GACGC(5/10) 137 GCGTC HphI 486 GGTGA GGTGA(8/7) 269 TCACC RsaI 309 GTAC GT^AC 354 GTAC SmaI 168 CCCGGG CCC^GGG 454 CCCGGG TspRI 440 CAGTG CA[GC]TG[ACGT][ACGT]^ 510 CACTG XmaCI 168 CCCGGG C^CCGGG 454 CCCGGG XmaI 168 CCCGGG C^CCGGG 454 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AccII AciI AcyI AluI BcnI BsaHI BsaJI BsiYI BslI BsmFI BssKI BstUI CfoI Cfr13I DdeI EcoT14I Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MaeII MnlI MspI MvnI MwoI NciI NlaIV Sau96I ScrFI StyI