################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AviII AvrII BbsI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MroI MunI NcoI NdeI NheI NruI NsiI NspV PacI PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Van91I XbaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 1087 GACGTC GACGT^C AccI 1090 GTCTAC GT^[AC][GT]AC AhdI 1012 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1012 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 232 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 29 GGATCC G^GATCC BbrPI 361 CACGTG CAC^GTG BbvCI 321 CCTCAGC CCTCAGC(-5/-2) BcgI 1415 GCAAGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 447 TGATCA T^GATCA BpmI 340 CTGGAG CTGGAG(16/14) BsaAI 361 CACGTG [CT]AC^GT[GA] BsaI 763 GGTCTC GGTCTC(1/5) BsgI 1441 CTGCAC GTGCAG(16/14) BsmAI 764 GTCTC GTCTC(1/5) BspMI 1439 ACCTGC ACCTGC(4/8) Eam1105I 1012 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 114 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 447 TGATCA T^GATCA MseI 1613 TTAA T^TAA MslI 1368 CATGCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PaeR7I 481 CTCGAG C^TCGAG PflFI 232 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 361 CACGTG CAC^GTG PmlI 361 CACGTG CAC^GTG PshAI 1419 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfaNI 586 GATGC GCATC(5/9) StuI 1136 AGGCCT AGG^CCT Tru9I 1613 TTAA T^TAA Tsp509I 444 AATT ^AATT Tth111I 232 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 593 CCAAGGAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 481 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 218 ACGTGT A^C[GA][CT]GT 362 ACGTGT Alw44I 1279 GTGCAC G^TGCAC 1375 GTGCAC ApaI 666 GGGCCC GGGCC^C 922 GGGCCC ApaLI 1279 GTGCAC G^TGCAC 1375 GTGCAC Asp700I 180 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1059 GAAGTACTTC AspHI 1279 GTGCAC G[AT]GC[AT]^C 1375 GTGCAC BalI 730 TGGCCA TGG^CCA 1640 TGGCCA BanII 666 GGGCCC G[GA]GC[CT]^C 922 GGGCCC BsiHKAI 1279 GTGCAC G[AT]GC[AT]^C 1375 GTGCAC BsrI 168 CCAGT ACTGG(1/-1) 1398 CCAGT BstAPI 87 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1235 GCAACATCTGC DraII 89 AGGGCCT [GA]G^G[ACGT]CC[CT] 666 GGGCCCC DrdI 1033 GACAAGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1081 GACGGCGACGTC EcoO109I 89 AGGGCCT [GA]G^G[ACGT]CC[CT] 666 GGGCCCC MluNI 730 TGGCCA TGG^CCA 1640 TGGCCA MscI 730 TGGCCA TGG^CCA 1640 TGGCCA NotI 523 GCGGCCGC GC^GGCCGC 1490 GCGGCCGC NspI 1283 ACATGC [GA]CATG^[CT] 1367 ACATGC PstI 162 CTGCAG CTGCA^G 958 CTGCAG PvuII 424 CAGCTG CAG^CTG 1407 CAGCTG ScaI 170 AGTACT AGT^ACT 1061 AGTACT SfcI 162 CTGCAG C^T[GA][CT]AG 958 CTGCAG SgrAI 776 CGCCGGTG C[GA]^CCGG[CT]G 1680 CGCCGGTG SmlI 481 CTCGAG C^T[CT][GA]AG 1453 CTCAAG TaqI 230 TCGA T^CGA 482 TCGA Tsp45I 314 GTGAC ^GT[GC]AC 1694 GTGAC TspRI 1324 CAGTG CA[GC]TG[ACGT][ACGT]^ 1399 CAGTG TthHB8I 230 TCGA T^CGA 482 TCGA XmnI 180 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1059 GAAGTACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BanI BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI RsaI SacII Sau3AI Sau96I ScrFI SfiI SfoI SmaI StyI TseI XhoII XmaCI XmaI