(Circular) (Six-base) MAPSORT of: pFN21AB7056.seq Check: 2263 from: 1 to: 6457 pFN21AB7056 6457 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3453 3453 Size: 6457 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3096 4267 278 Size: 53 83 186 936 1560 1171 2468 Fragments arranged by size: 2468 1560 1171 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3096 3800 1937 Size: 1159 704 4594 Fragments arranged by size: 4594 1159 704 Acc65I G'GTAC_C Cuts at: 3778 3778 Size: 6457 AclI AA'CG_TT Cuts at: 1563 4584 4957 1563 Size: 3021 373 3063 Fragments arranged by size: 3063 3021 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3055 3083 3299 3383 4520 5904 6172 1632 Size: 1423 28 216 84 1137 1384 268 1917 Fragments arranged by size: 1917 1423 1384 1137 268 216 84 28 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6260 AflIII A'CryG_T Cuts at: 1203 2223 2367 5356 6333 1203 Size: 1020 144 2989 977 1327 Fragments arranged by size: 2989 1327 1020 977 144 AgeI A'CCGG_T Cuts at: 5288 5288 Size: 6457 AhdI GACnn_n'nnGTC Cuts at: 3022 5186 3022 Size: 2164 4293 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6457 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2978 1184 Size: 1794 4663 AlwNI CAG_nnn'CTG Cuts at: 2098 2398 3417 4200 5772 2098 Size: 300 1019 783 1572 2783 Fragments arranged by size: 2783 1572 1019 783 300 ApaI G_GGCC'C Cuts at: 2675 2931 2675 Size: 256 6201 ApaLI G'TGCA_C Cuts at: 3284 3380 4514 5670 3284 Size: 96 1134 1156 4071 Fragments arranged by size: 4071 1156 1134 96 ApoI r'AATT_y Cuts at: 1493 3766 3992 4144 6253 1493 Size: 2273 226 152 2109 1697 Fragments arranged by size: 2273 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5011 160 Size: 872 3979 1606 Fragments arranged by size: 3979 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 AvaI C'yCGr_G Cuts at: 1870 1943 2486 2725 2867 3538 3716 3782 1870 Size: 73 543 239 142 671 178 66 4545 Fragments arranged by size: 4545 671 543 239 178 142 73 66 BamHI G'GATC_C Cuts at: 2034 3787 4154 2034 Size: 1753 367 4337 Fragments arranged by size: 4337 1753 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2201 2414 3077 3182 3335 Size: 325 217 627 414 213 663 105 153 Cuts at: 3335 3523 3560 3683 3778 5233 618 Size: 188 37 123 95 1455 1842 Fragments arranged by size: 1842 1455 663 627 414 325 217 213 188 153 123 105 95 37 BanII G_rGCy'C Cuts at: 1391 2675 2931 3776 1391 Size: 1284 256 845 4072 Fragments arranged by size: 4072 1284 845 256 BbeI G_GCGC'C Cuts at: 2205 2418 3081 3186 3339 3527 3564 3687 2205 Size: 213 663 105 153 188 37 123 4975 Fragments arranged by size: 4975 663 213 188 153 123 105 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5486 BbvCI CC'TCA_GC Cuts at: 2327 2327 Size: 6457 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3409 3443 4648 4682 1903 Size: 34 1472 34 1205 34 3678 Fragments arranged by size: 3678 1472 1205 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4349 5559 4349 Size: 1210 5247 BclI T'GATC_A Cuts at: 1548 2452 1548 Size: 904 5553 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2285 2570 2621 Size: 107 122 71 631 605 613 285 51 Cuts at: 2621 2630 2795 2820 3657 5068 136 Size: 9 165 25 837 1411 1525 Fragments arranged by size: 1525 1411 837 631 613 605 285 165 122 107 71 51 25 9 BglII A'GATC_T Cuts at: 6452 6452 Size: 6457 BlpI GC'TnA_GC Cuts at: 3874 3874 Size: 6457 Bme1580I G_kGCm'C Cuts at: 1790 2675 2931 3158 3288 3384 4518 5674 1790 Size: 885 256 227 130 96 1134 1156 2573 Fragments arranged by size: 2573 1156 1134 885 256 227 130 96 BmgBI CAC'GTC Cuts at: 1318 2110 2196 2935 3643 1318 Size: 792 86 739 708 4132 Fragments arranged by size: 4132 792 739 708 86 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4188 5146 442 Size: 416 53 83 399 24 2771 958 1753 Fragments arranged by size: 2771 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6457 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2863 2863 Size: 6457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 5117 1239 Size: 1127 2751 2579 Fragments arranged by size: 2751 2579 1127 Bpu10I CC'TnA_GC Cuts at: 2327 2327 Size: 6457 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3443 4582 5447 5745 3443 Size: 1139 865 298 4155 Fragments arranged by size: 4155 1139 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2774 5120 882 Size: 617 1275 2346 2219 Fragments arranged by size: 2346 2219 1275 617 BsaAI yAC'GTr Cuts at: 493 1204 2368 4248 493 Size: 711 1164 1880 2702 Fragments arranged by size: 2702 1880 1164 711 BsaBI GATnn'nnATC Cuts at: 6451 6451 Size: 6457 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2202 2415 2421 2573 Size: 53 83 186 936 669 213 6 152 Cuts at: 2573 2754 3045 3078 3093 3183 3336 3480 3524 Size: 181 291 33 15 90 153 144 44 Cuts at: 3524 3561 3588 3684 4264 4646 5281 275 Size: 37 27 96 580 382 635 1451 Fragments arranged by size: 1451 936 669 635 580 382 291 213 186 181 153 152 144 96 90 83 53 44 37 33 27 15 6 BsaWI w'CCGG_w Cuts at: 4890 5288 5562 5709 6120 4890 Size: 398 274 147 411 5227 Fragments arranged by size: 5227 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6232 Fragments arranged by size: 6232 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 2849 2852 3035 3708 1186 Size: 1270 393 3 183 673 3935 Fragments arranged by size: 3935 1270 673 393 183 3 BseYI C'CCAG_C Cuts at: 3533 3570 3575 5660 6156 3533 Size: 37 5 2085 496 3834 Fragments arranged by size: 3834 2085 496 37 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3431 1163 Size: 573 1695 4189 Fragments arranged by size: 4189 1695 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2406 2532 2682 2799 2824 2907 Size: 275 186 405 126 150 117 25 83 Cuts at: 2907 3499 3907 4668 5696 1540 Size: 592 408 761 1028 2301 Fragments arranged by size: 2301 1028 761 592 408 405 275 186 150 126 117 83 25 BsiHKAI G_wGCw'C Cuts at: 3288 3384 4518 4603 5674 3288 Size: 96 1134 85 1071 4071 Fragments arranged by size: 4071 1134 1071 96 85 BsmI GAATG_Cn' Cuts at: 3968 4061 3968 Size: 93 6364 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2675 2931 3158 3288 3384 3776 4518 Size: 399 885 256 227 130 96 392 742 Cuts at: 4518 4603 5674 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 885 742 399 392 256 227 130 96 85 BspEI T'CCGG_A Cuts at: 6120 6120 Size: 6457 BspHI T'CATG_A Cuts at: 4346 6076 4346 Size: 1730 4727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3453 3812 844 Size: 2609 359 3489 Fragments arranged by size: 3489 2609 359 BsrBI CCG'CTC Cuts at: 1394 1823 2151 2686 2743 2903 3435 4344 1394 Size: 429 328 535 57 160 532 909 3507 Fragments arranged by size: 3507 909 535 532 429 328 160 57 BsrDI GCAATG_nn' Cuts at: 65 1248 4952 5126 65 Size: 1183 3704 174 1396 Fragments arranged by size: 3704 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2388 2782 2794 3185 3666 3686 Size: 110 199 379 394 12 391 481 20 Cuts at: 3686 5101 5288 1700 Size: 1415 187 2869 Fragments arranged by size: 2869 1415 481 394 391 379 199 187 110 20 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6457 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6457 BssSI C'ACGA_G Cuts at: 4517 5529 4517 Size: 1012 5445 BstAPI GCAn_nnn'nTGC Cuts at: 794 2098 3246 794 Size: 1304 1148 4005 Fragments arranged by size: 4005 1304 1148 BstBI TT'CG_AA Cuts at: 5261 5261 Size: 6457 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6457 BstYI r'GATC_y Cuts at: 1972 2034 2349 2394 3129 3787 4154 4539 4556 Size: 62 315 45 735 658 367 385 17 Cuts at: 4556 6008 6094 6106 6452 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 735 658 385 367 346 315 86 62 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2205 2526 2555 2607 2628 2634 Size: 552 939 201 321 29 52 21 6 Cuts at: 2634 2824 3000 3493 513 Size: 190 176 493 3477 Fragments arranged by size: 3477 939 552 493 321 201 190 176 52 29 21 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5718 Fragments arranged by size: 5718 683 56 BtsI GCAGTG_nn' Cuts at: 3335 3973 4767 4787 3335 Size: 638 794 20 5005 Fragments arranged by size: 5005 794 638 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6457 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6422 DraI TTT'AAA Cuts at: 3761 3761 Size: 6457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3044 3092 5464 809 Size: 507 1728 48 2372 1802 Fragments arranged by size: 2372 1802 1728 507 48 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2160 2286 Size: 54 1425 50 275 18 177 153 126 Cuts at: 2286 2403 2529 2610 2622 2631 2679 2735 2796 Size: 117 126 81 12 9 48 56 61 Cuts at: 2796 2812 2821 2904 2949 3496 3601 3645 3904 Size: 16 9 83 45 547 105 44 259 Cuts at: 3904 4793 6422 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 547 275 259 177 153 126 126 117 105 83 81 61 56 54 50 48 45 44 43 18 16 12 9 9 EagI C'GGCC_G Cuts at: 1537 1812 2403 2529 2679 2796 2821 2904 3496 Size: 275 591 126 150 117 25 83 592 Cuts at: 3496 3904 1537 Size: 408 4090 Fragments arranged by size: 4090 592 591 408 275 150 126 117 83 25 EarI CTCTTCn'nnn_ Cuts at: 1353 4387 1353 Size: 3034 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2551 2766 2844 5044 5418 5564 Size: 1064 138 962 215 78 2200 374 146 Cuts at: 5564 387 Size: 1280 Fragments arranged by size: 2200 1280 1064 962 374 215 146 138 78 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 3055 3083 3299 3383 4520 5117 Size: 393 734 689 28 216 84 1137 597 Cuts at: 5117 5904 6172 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1137 787 734 689 597 393 268 216 84 28 EcoNI CCTnn'n_nnAGG Cuts at: 2123 2123 Size: 6457 EcoO109I rG'GnC_Cy Cuts at: 2095 2672 2095 Size: 577 5880 EcoRI G'AATT_C Cuts at: 3766 4144 3766 Size: 378 6079 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3274 3274 Size: 6457 FspI TGC'GCA Cuts at: 4963 4963 Size: 6457 HaeII r_GCGC'y Cuts at: 1678 2205 2250 2258 2334 2418 3081 3186 3339 Size: 527 45 8 76 84 663 105 153 Cuts at: 3339 3527 3564 3687 5604 1678 Size: 188 37 123 1917 2531 Fragments arranged by size: 2531 1917 663 527 188 153 123 105 84 76 45 37 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3008 3040 5098 5130 5172 Size: 32 135 32 1436 32 2058 32 42 Cuts at: 5172 5204 1373 Size: 32 2626 Fragments arranged by size: 2626 2058 1436 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3801 4047 669 Size: 988 281 1863 246 3079 Fragments arranged by size: 3079 1863 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6457 HpaI GTT'AAC Cuts at: 4047 4047 Size: 6457 KasI G'GCGC_C Cuts at: 2201 2414 3077 3182 3335 3523 3560 3683 2201 Size: 213 663 105 153 188 37 123 4975 Fragments arranged by size: 4975 663 213 188 153 123 105 37 KpnI G_GTAC'C Cuts at: 3782 3782 Size: 6457 MfeI C'AATT_G Cuts at: 4056 4056 Size: 6457 MluI A'CGCG_T Cuts at: 6333 6333 Size: 6457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2835 3462 5571 5755 1305 Size: 1530 627 2109 184 2007 Fragments arranged by size: 2109 2007 1530 627 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2737 3647 10 Size: 54 1425 343 905 910 2820 Fragments arranged by size: 2820 1425 910 905 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3377 4415 4774 4933 518 Size: 648 2211 1038 359 159 2042 Fragments arranged by size: 2211 2042 1038 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2086 2431 2528 2543 2618 2636 2826 3002 Size: 480 345 97 15 75 18 190 176 Cuts at: 3002 3075 3414 3495 3705 3873 4550 5698 5943 Size: 73 339 81 210 168 677 1148 245 Cuts at: 5943 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 480 345 339 245 210 190 176 168 97 81 75 73 18 15 NaeI GCC'GGC Cuts at: 2011 2796 3187 2011 Size: 785 391 5281 Fragments arranged by size: 5281 785 391 NarI GG'CG_CC Cuts at: 2202 2415 3078 3183 3336 3524 3561 3684 2202 Size: 213 663 105 153 188 37 123 4975 Fragments arranged by size: 4975 663 213 188 153 123 105 37 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4966 Fragments arranged by size: 4966 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6457 NgoMIV G'CCGG_C Cuts at: 2009 2794 3185 2009 Size: 785 391 5281 Fragments arranged by size: 5281 785 391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6457 NotI GC'GGCC_GC Cuts at: 2529 3496 3904 2529 Size: 967 408 5082 Fragments arranged by size: 5082 967 408 NspI r_CATG'y Cuts at: 1132 1229 3292 3376 3815 5360 1132 Size: 97 2063 84 439 1545 2229 Fragments arranged by size: 2229 2063 1545 439 97 84 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6457 PciI A'CATG_T Cuts at: 5356 5356 Size: 6457 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6457 PfoI T'CCnGG_A Cuts at: 2382 2382 Size: 6457 PmeI GTTT'AAAC Cuts at: 3761 3761 Size: 6457 PmlI CAC'GTG Cuts at: 2368 2368 Size: 6457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4526 4558 6039 6071 4526 Size: 32 1481 32 4912 Fragments arranged by size: 4912 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3428 1613 Size: 1815 4642 PsiI TTA'TAA Cuts at: 4027 4027 Size: 6457 PspOMI G'GGCC_C Cuts at: 2671 2927 2671 Size: 256 6201 PspXI vC'TCGA_Gb Cuts at: 2486 2486 Size: 6457 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2474 2506 2801 2833 2474 Size: 32 295 32 6098 Fragments arranged by size: 6098 295 32 32 PstI C_TGCA'G Cuts at: 830 2171 2967 3809 830 Size: 1341 796 842 3478 Fragments arranged by size: 3478 1341 842 796 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6457 PvuII CAG'CTG Cuts at: 2431 3414 2431 Size: 983 5474 SacII CC_GC'GG Cuts at: 2529 2637 2827 3003 3496 2529 Size: 108 190 176 493 5490 Fragments arranged by size: 5490 493 190 176 108 SalI G'TCGA_C Cuts at: 1936 3799 1936 Size: 1863 4594 SbfI CC_TGCA'GG Cuts at: 3809 3809 Size: 6457 ScaI AGT'ACT Cuts at: 2177 3068 4705 6417 2177 Size: 891 1637 1712 2217 Fragments arranged by size: 2217 1712 1637 891 SfcI C'TryA_G Cuts at: 826 1045 2167 2963 3805 4940 5621 5812 826 Size: 219 1122 796 842 1135 681 191 1471 Fragments arranged by size: 1471 1135 1122 842 796 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2630 2795 2820 2630 Size: 165 25 6267 Fragments arranged by size: 6267 165 25 SfoI GGC'GCC Cuts at: 2203 2416 3079 3184 3337 3525 3562 3685 2203 Size: 213 663 105 153 188 37 123 4975 Fragments arranged by size: 4975 663 213 188 153 123 105 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 SgrAI Cr'CCGG_yG Cuts at: 1700 2782 3686 1700 Size: 1082 904 4471 Fragments arranged by size: 4471 1082 904 SmaI CCC'GGG Cuts at: 1872 2727 3540 3718 3784 1872 Size: 855 813 178 66 4545 Fragments arranged by size: 4545 855 813 178 66 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 3458 4561 5462 5724 820 Size: 197 926 543 972 1103 901 262 1553 Fragments arranged by size: 1553 1103 972 926 901 543 262 197 SnaBI TAC'GTA Cuts at: 493 4248 493 Size: 3755 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6457 SphI G_CATG'C Cuts at: 1132 3815 1132 Size: 2683 3774 SspI AAT'ATT Cuts at: 5 52 4381 5 Size: 47 4329 2081 Fragments arranged by size: 4329 2081 47 StuI AGG'CCT Cuts at: 1517 3143 1517 Size: 1626 4831 StyI C'CwwG_G Cuts at: 513 1065 2004 2475 2598 3138 3896 513 Size: 552 939 471 123 540 758 3074 Fragments arranged by size: 3074 939 758 552 540 471 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 3227 4484 4501 4654 4839 5284 Size: 1255 726 921 1257 17 153 185 445 Cuts at: 5284 325 Size: 1498 Fragments arranged by size: 1498 1257 1255 921 726 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 3066 4703 Size: 274 80 33 51 1443 198 891 1637 Cuts at: 4703 6415 96 Size: 1712 138 Fragments arranged by size: 1712 1637 1443 891 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6417 6449 6417 Size: 32 6425 Tth111I GACn'n_nGTC Cuts at: 1316 2240 1316 Size: 924 5533 XbaI T'CTAG_A Cuts at: 3793 3793 Size: 6457 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 2605 Size: 6457 XhoI C'TCGA_G Cuts at: 1943 2486 1943 Size: 543 5914 XmaI C'CCGG_G Cuts at: 1870 2725 3538 3716 3782 1870 Size: 855 813 178 66 4545 Fragments arranged by size: 4545 855 813 178 66 XmnI GAAnn'nnTTC Cuts at: 2189 3068 4586 2189 Size: 879 1518 4060 Fragments arranged by size: 4060 1518 879 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3094 4265 276 Size: 53 83 186 936 1560 1171 2468 Fragments arranged by size: 2468 1560 1171 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PpuMI RsrII SacI SanDI SapI SexAI SrfI SwaI