(Linear) (Six-base) MAPSORT of: KIBB7056.seq Check: 6207 from: 1 to: 1752 KIBB7056.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1448 1752 Size: 1448 304 AatII G_ACGT'C Cuts at: 0 1091 1752 Size: 1091 661 AccI GT'mk_AC Cuts at: 0 1091 1752 Size: 1091 661 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1050 1078 1294 1378 1752 Size: 1050 28 216 84 374 Fragments arranged by size: 1050 374 216 84 28 AflIII A'CryG_T Cuts at: 0 218 362 1752 Size: 218 144 1390 Fragments arranged by size: 1390 218 144 AhdI GACnn_n'nnGTC Cuts at: 0 1017 1752 Size: 1017 735 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 973 1752 Size: 973 779 AlwNI CAG_nnn'CTG Cuts at: 0 93 393 1412 1752 Size: 93 300 1019 340 Fragments arranged by size: 1019 340 300 93 ApaI G_GGCC'C Cuts at: 0 670 926 1752 Size: 670 256 826 Fragments arranged by size: 826 670 256 ApaLI G'TGCA_C Cuts at: 0 1279 1375 1752 Size: 1279 96 377 Fragments arranged by size: 1279 377 96 AvaI C'yCGr_G Cuts at: 0 481 720 862 1533 1711 1752 Size: 481 239 142 671 178 41 Fragments arranged by size: 671 481 239 178 142 41 BamHI G'GATC_C Cuts at: 0 29 1752 Size: 29 1723 BanI G'GyrC_C Cuts at: 0 196 409 1072 1177 1330 1518 1555 1678 Size: 196 213 663 105 153 188 37 123 Cuts at: 1678 1752 Size: 74 Fragments arranged by size: 663 213 196 188 153 123 105 74 37 BanII G_rGCy'C Cuts at: 0 670 926 1752 Size: 670 256 826 Fragments arranged by size: 826 670 256 BbeI G_GCGC'C Cuts at: 0 200 413 1076 1181 1334 1522 1559 1682 Size: 200 213 663 105 153 188 37 123 Cuts at: 1682 1752 Size: 70 Fragments arranged by size: 663 213 200 188 153 123 105 70 37 BbvCI CC'TCA_GC Cuts at: 0 322 1752 Size: 322 1430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1404 1438 1752 Size: 1404 34 314 Fragments arranged by size: 1404 314 34 BclI T'GATC_A Cuts at: 0 447 1752 Size: 447 1305 BglI GCCn_nnn'nGGC Cuts at: 0 280 565 616 625 790 815 1652 1752 Size: 280 285 51 9 165 25 837 100 Fragments arranged by size: 837 285 280 165 100 51 25 9 Bme1580I G_kGCm'C Cuts at: 0 670 926 1153 1283 1379 1752 Size: 670 256 227 130 96 373 Fragments arranged by size: 670 373 256 227 130 96 BmgBI CAC'GTC Cuts at: 0 105 191 930 1638 1752 Size: 105 86 739 708 114 Fragments arranged by size: 739 708 114 105 86 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 858 1752 Size: 858 894 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 1752 Size: 361 1391 Bpu10I CC'TnA_GC Cuts at: 0 322 1752 Size: 322 1430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1438 1752 Size: 1438 314 BsaI GGTCTCn'nnnn_ Cuts at: 0 769 1752 Size: 769 983 BsaAI yAC'GTr Cuts at: 0 363 1752 Size: 363 1389 BsaHI Gr'CG_yC Cuts at: 0 197 410 416 568 749 1040 1073 1088 Size: 197 213 6 152 181 291 33 15 Cuts at: 1088 1178 1331 1475 1519 1556 1583 1679 1752 Size: 90 153 144 44 37 27 96 73 Fragments arranged by size: 291 213 197 181 153 152 144 96 90 73 44 37 33 27 15 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 451 844 847 1030 1703 1752 Size: 451 393 3 183 673 49 Fragments arranged by size: 673 451 393 183 49 3 BseYI C'CCAG_C Cuts at: 0 1528 1565 1570 1752 Size: 1528 37 5 182 Fragments arranged by size: 1528 182 37 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1426 1752 Size: 1426 326 BsiEI CG_ry'CG Cuts at: 0 401 527 677 794 819 902 1494 1752 Size: 401 126 150 117 25 83 592 258 Fragments arranged by size: 592 401 258 150 126 117 83 25 BsiHKAI G_wGCw'C Cuts at: 0 1283 1379 1752 Size: 1283 96 373 Fragments arranged by size: 1283 373 96 Bsp1286I G_dGCh'C Cuts at: 0 670 926 1153 1283 1379 1752 Size: 670 256 227 130 96 373 Fragments arranged by size: 670 373 256 227 130 96 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1448 1752 Size: 1448 304 BsrBI CCG'CTC Cuts at: 0 146 681 738 898 1430 1752 Size: 146 535 57 160 532 322 Fragments arranged by size: 535 532 322 160 146 57 BsrFI r'CCGG_y Cuts at: 0 4 383 777 789 1180 1661 1681 1752 Size: 4 379 394 12 391 481 20 71 Fragments arranged by size: 481 394 391 379 71 20 12 4 BstAPI GCAn_nnn'nTGC Cuts at: 0 93 1241 1752 Size: 93 1148 511 Fragments arranged by size: 1148 511 93 BstYI r'GATC_y Cuts at: 0 29 344 389 1124 1752 Size: 29 315 45 735 628 Fragments arranged by size: 735 628 315 45 29 BtgI C'CryG_G Cuts at: 0 200 521 550 602 623 629 819 995 Size: 200 321 29 52 21 6 190 176 Cuts at: 995 1488 1752 Size: 493 264 Fragments arranged by size: 493 321 264 200 190 176 52 29 21 6 BtsI GCAGTG_nn' Cuts at: 0 1330 1752 Size: 1330 422 DrdI GACnn_nn'nnGTC Cuts at: 0 1039 1087 1752 Size: 1039 48 665 Fragments arranged by size: 1039 665 48 EaeI y'GGCC_r Cuts at: 0 2 155 281 398 524 605 617 626 Size: 2 153 126 117 126 81 12 9 Cuts at: 626 674 730 791 807 816 899 944 1491 Size: 48 56 61 16 9 83 45 547 Cuts at: 1491 1596 1640 1752 Size: 105 44 112 Fragments arranged by size: 547 153 126 126 117 112 105 83 81 61 56 48 45 44 16 12 9 9 2 EagI C'GGCC_G Cuts at: 0 398 524 674 791 816 899 1491 1752 Size: 398 126 150 117 25 83 592 261 Fragments arranged by size: 592 398 261 150 126 117 83 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 546 761 839 1752 Size: 546 215 78 913 Fragments arranged by size: 913 546 215 78 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 1050 1078 1294 1378 1752 Size: 361 689 28 216 84 374 Fragments arranged by size: 689 374 361 216 84 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 118 1752 Size: 118 1634 EcoO109I rG'GnC_Cy Cuts at: 0 90 667 1752 Size: 90 577 1085 Fragments arranged by size: 1085 577 90 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1269 1752 Size: 1269 483 HaeII r_GCGC'y Cuts at: 0 200 245 253 329 413 1076 1181 1334 Size: 200 45 8 76 84 663 105 153 Cuts at: 1334 1522 1559 1682 1752 Size: 188 37 123 70 Fragments arranged by size: 663 200 188 153 123 105 84 76 70 45 37 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1003 1035 1752 Size: 1003 32 717 Fragments arranged by size: 1003 717 32 KasI G'GCGC_C Cuts at: 0 196 409 1072 1177 1330 1518 1555 1678 Size: 196 213 663 105 153 188 37 123 Cuts at: 1678 1752 Size: 74 Fragments arranged by size: 663 213 196 188 153 123 105 74 37 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 830 1457 1752 Size: 830 627 295 Fragments arranged by size: 830 627 295 MscI TGG'CCA Cuts at: 0 732 1642 1752 Size: 732 910 110 Fragments arranged by size: 910 732 110 MslI CAynn'nnrTG Cuts at: 0 1372 1752 Size: 1372 380 MspA1I CmG'CkG Cuts at: 0 81 426 523 538 613 631 821 997 Size: 81 345 97 15 75 18 190 176 Cuts at: 997 1070 1409 1490 1700 1752 Size: 73 339 81 210 52 Fragments arranged by size: 345 339 210 190 176 97 81 81 75 73 52 18 15 NaeI GCC'GGC Cuts at: 0 6 791 1182 1752 Size: 6 785 391 570 Fragments arranged by size: 785 570 391 6 NarI GG'CG_CC Cuts at: 0 197 410 1073 1178 1331 1519 1556 1679 Size: 197 213 663 105 153 188 37 123 Cuts at: 1679 1752 Size: 73 Fragments arranged by size: 663 213 197 188 153 123 105 73 37 NgoMIV G'CCGG_C Cuts at: 0 4 789 1180 1752 Size: 4 785 391 572 Fragments arranged by size: 785 572 391 4 NotI GC'GGCC_GC Cuts at: 0 524 1491 1752 Size: 524 967 261 Fragments arranged by size: 967 524 261 NspI r_CATG'y Cuts at: 0 1287 1371 1752 Size: 1287 84 381 Fragments arranged by size: 1287 381 84 PfoI T'CCnGG_A Cuts at: 0 377 1752 Size: 377 1375 PmlI CAC'GTG Cuts at: 0 363 1752 Size: 363 1389 PshAI GACnn'nnGTC Cuts at: 0 1423 1752 Size: 1423 329 PspOMI G'GGCC_C Cuts at: 0 666 922 1752 Size: 666 256 830 Fragments arranged by size: 830 666 256 PspXI vC'TCGA_Gb Cuts at: 0 481 1752 Size: 481 1271 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 469 501 796 828 1752 Size: 469 32 295 32 924 Fragments arranged by size: 924 469 295 32 32 PstI C_TGCA'G Cuts at: 0 166 962 1752 Size: 166 796 790 Fragments arranged by size: 796 790 166 PvuII CAG'CTG Cuts at: 0 426 1409 1752 Size: 426 983 343 Fragments arranged by size: 983 426 343 SacII CC_GC'GG Cuts at: 0 524 632 822 998 1491 1752 Size: 524 108 190 176 493 261 Fragments arranged by size: 524 493 261 190 176 108 ScaI AGT'ACT Cuts at: 0 172 1063 1752 Size: 172 891 689 Fragments arranged by size: 891 689 172 SfcI C'TryA_G Cuts at: 0 162 958 1752 Size: 162 796 794 Fragments arranged by size: 796 794 162 SfiI GGCCn_nnn'nGGCC Cuts at: 0 625 790 815 1752 Size: 625 165 25 937 Fragments arranged by size: 937 625 165 25 SfoI GGC'GCC Cuts at: 0 198 411 1074 1179 1332 1520 1557 1680 Size: 198 213 663 105 153 188 37 123 Cuts at: 1680 1752 Size: 72 Fragments arranged by size: 663 213 198 188 153 123 105 72 37 SgrAI Cr'CCGG_yG Cuts at: 0 777 1681 1752 Size: 777 904 71 Fragments arranged by size: 904 777 71 SmaI CCC'GGG Cuts at: 0 722 1535 1713 1752 Size: 722 813 178 39 Fragments arranged by size: 813 722 178 39 SmlI C'TyrA_G Cuts at: 0 481 1453 1752 Size: 481 972 299 Fragments arranged by size: 972 481 299 StuI AGG'CCT Cuts at: 0 1138 1752 Size: 1138 614 StyI C'CwwG_G Cuts at: 0 470 593 1133 1752 Size: 470 123 540 619 Fragments arranged by size: 619 540 470 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 301 1222 1752 Size: 301 921 530 Fragments arranged by size: 921 530 301 TatI w'GTAC_w Cuts at: 0 170 1061 1752 Size: 170 891 691 Fragments arranged by size: 891 691 170 Tth111I GACn'n_nGTC Cuts at: 0 235 1752 Size: 235 1517 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 600 1752 Size: 600 1152 XhoI C'TCGA_G Cuts at: 0 481 1752 Size: 481 1271 XmaI C'CCGG_G Cuts at: 0 720 1533 1711 1752 Size: 720 813 178 41 Fragments arranged by size: 813 720 178 41 XmnI GAAnn'nnTTC Cuts at: 0 184 1063 1752 Size: 184 879 689 Fragments arranged by size: 879 689 184 ZraI GAC'GTC Cuts at: 0 1089 1752 Size: 1089 663 Enzymes that do cut: AarI AatII AccI AcuI AflIII AhdI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI BcgI BclI BglI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI BtgI BtsI DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PfoI PmlI PshAI PspOMI PspXI PsrI PstI PvuII SacII ScaI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI BfrBI BglII BlpI BmrI BmtI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EarI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PpuMI PsiI PvuI RsrII SacI SalI SanDI SapI SbfI SexAI SgfI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI