################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EarI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MaeI MaeIII MamI MfeI MroI MslI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tsp509I TspRI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 1097 GGTACC G^GTACC AcyI 884 GGCGCC G[GA]^CG[CT]C AhdI 894 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 968 AGCGCT AGC^GCT Asp718I 1097 GGTACC G^GTACC AspEI 894 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 298 GAGCTC G[AT]GC[AT]^C AspI 262 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 874 CCCGAG C^[CT]CG[GA]G BanII 298 GAGCTC G[GA]GC[CT]^C BbeI 884 GGCGCC GGCGC^C BbsI 891 GAAGAC GAAGAC(2/6) BglI 873 GCCCGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 891 GAAGAC GAAGAC(2/6) BsaHI 884 GGCGCC G[GA]^CG[CT]C BsiHKAI 298 GAGCTC G[AT]GC[AT]^C BsmBI 670 GAGACG CGTCTC(1/5) BsmFI 1213 GGGAC GGGAC(10/14) BsoBI 874 CCCGAG C^[CT]CG[GA]G BspLU11I 596 ACATGT A^CATGT BsrDI 795 CATTGC GCAATG(2/0) BstAPI 1146 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Eam1105I 894 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 968 AGCGCT AGC^GCT Hin1I 884 GGCGCC G[GA]^CG[CT]C KasI 884 GGCGCC G^GCGCC KpnI 1097 GGTACC GGTAC^C MluI 898 ACGCGT A^CGCGT MseI 824 TTAA T^TAA NarI 884 GGCGCC GG^CGCC NspI 596 ACATGT [GA]CATG^[CT] PflFI 262 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 801 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 325 GAGTC GAGTC(4/5) PvuI 74 CGATCG CGAT^CG SacI 298 GAGCTC GAGCT^C ScaI 716 AGTACT AGT^ACT SfoI 884 GGCGCC GGC^GCC StuI 1091 AGGCCT AGG^CCT TfiI 467 GAATC G^A[AT]TC Tru9I 824 TTAA T^TAA Tth111I 262 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 801 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 596 ACATGT A^C[GA][CT]GT 898 ACGCGT Asp700I 513 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 525 GAACATCTTC BalI 740 TGGCCA TGG^CCA 1268 TGGCCA BcnI 171 CCGGG CC^[GC]GG 540 CCCGG BsaI 658 GAGACC GGTCTC(1/5) 285 GGTCTC BspMI 386 ACCTGC ACCTGC(4/8) 1130 ACCTGC BsrI 66 CCAGT ACTGG(1/-1) 351 CCAGT BssHII 1202 GCGCGC G^CGCGC 1314 GCGCGC BssSI 1048 CACGAG CACGAG(-5/-1) 1309 CTCGTG BstXI 731 CCAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 977 CCAAGGTGCTGG EagI 864 CGGCCG C^GGCCG 998 CGGCCG EclXI 864 CGGCCG C^GGCCG 998 CGGCCG Eco52I 864 CGGCCG C^GGCCG 998 CGGCCG EcoNI 1119 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1320 CCTGGAGCAGG HgaI 672 GACGC GACGC(5/10) 900 GCGTC HinfI 325 GAGTC G^A[ACGT]TC 467 GAATC MluNI 740 TGGCCA TGG^CCA 1268 TGGCCA MscI 740 TGGCCA TGG^CCA 1268 TGGCCA NciI 171 CCGGG CC^[GC]GG 540 CCCGG NlaIII 435 CATG CATG^ 597 CATG NotI 863 GCGGCCGC GC^GGCCGC 997 GCGGCCGC PpuMI 1127 AGGACCT [GA]G^G[AT]CC[CT] 1304 AGGACCT PstI 1024 CTGCAG CTGCA^G 1336 CTGCAG SfcI 1024 CTGCAG C^T[GA][CT]AG 1336 CTGCAG XmnI 513 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 525 GAACATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaII BanI BbvI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI Bsp1286I BsrBI BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NdeII NgoMIV NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TseI TthHB8I XhoII