(Circular) (Six-base) MAPSORT of: pFN21AB6670.seq Check: 7085 from: 1 to: 6067 pFN21AB6670 6067 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 1937 Size: 1473 4594 Acc65I G'GTAC_C Cuts at: 3102 3388 3102 Size: 286 5781 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2444 2710 2915 2930 3226 4130 5514 5782 Size: 812 266 205 15 296 904 1384 268 Cuts at: 5782 1632 Size: 1917 Fragments arranged by size: 1917 1384 904 812 296 268 266 205 15 AfeI AGC'GCT Cuts at: 2975 2975 Size: 6067 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 2601 2903 4966 5943 1203 Size: 1398 302 2063 977 1327 Fragments arranged by size: 2063 1398 1327 977 302 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 2904 4796 2904 Size: 1892 4175 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 2374 2812 3136 3157 3810 5382 2374 Size: 438 324 21 653 1572 3059 Fragments arranged by size: 3059 1572 653 438 324 21 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 3376 3602 3754 5863 1493 Size: 1883 226 152 2109 1697 Fragments arranged by size: 2109 1883 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2879 3392 1870 Size: 73 936 513 4545 Fragments arranged by size: 4545 936 513 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2220 2540 2889 3102 3388 Size: 325 217 627 433 320 349 213 286 Cuts at: 3388 4843 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 433 349 325 320 286 217 213 BanII G_rGCy'C Cuts at: 1391 2307 3386 1391 Size: 916 1079 4072 Fragments arranged by size: 4072 1079 916 BbeI G_GCGC'C Cuts at: 2893 2893 Size: 6067 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2903 928 Size: 971 1004 4092 Fragments arranged by size: 4092 1004 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2884 4678 136 Size: 107 122 71 631 605 1212 1794 1525 Fragments arranged by size: 1794 1525 1212 631 605 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 2108 2463 4128 5284 1790 Size: 318 355 1665 1156 2573 Fragments arranged by size: 2573 1665 1156 355 318 BmgBI CAC'GTC Cuts at: 1318 2029 2269 3061 1318 Size: 711 240 792 4324 Fragments arranged by size: 4324 792 711 240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2350 3798 4756 Size: 416 53 83 399 24 933 1448 958 Cuts at: 4756 442 Size: 1753 Fragments arranged by size: 1753 1448 958 933 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2308 2995 2308 Size: 687 5380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 2612 2920 3011 3272 3347 4727 1239 Size: 1064 309 308 91 261 75 1380 2579 Fragments arranged by size: 2579 1380 1064 309 308 261 91 75 Bpu10I CC'TnA_GC Cuts at: 2636 2636 Size: 6067 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2348 3146 3161 3215 4192 5057 5355 2348 Size: 798 15 54 977 865 298 3060 Fragments arranged by size: 3060 977 865 798 298 54 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2296 2657 4730 882 Size: 617 797 361 2073 2219 Fragments arranged by size: 2219 2073 797 617 361 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2890 3874 4256 4891 Size: 53 83 186 936 1357 984 382 635 Cuts at: 4891 275 Size: 1451 Fragments arranged by size: 1451 1357 984 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4500 4898 5172 5319 5730 4500 Size: 398 274 147 411 4837 Fragments arranged by size: 4837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2599 2629 3086 Size: 30 165 30 912 30 254 30 457 Cuts at: 3086 3116 1178 Size: 30 4129 Fragments arranged by size: 4129 912 457 254 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2195 2732 3008 3026 3101 3125 3209 Size: 883 126 537 276 18 75 24 84 Cuts at: 3209 1186 Size: 4044 Fragments arranged by size: 4044 883 537 276 126 84 75 24 18 BseYI C'CCAG_C Cuts at: 2059 2584 3214 5270 5766 2059 Size: 525 630 2056 496 2360 Fragments arranged by size: 2360 2056 630 525 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 2378 3326 1163 Size: 573 489 153 948 3904 Fragments arranged by size: 3904 948 573 489 153 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2082 2872 3006 3517 4278 5306 Size: 275 186 81 790 134 511 761 1028 Cuts at: 5306 1540 Size: 2301 Fragments arranged by size: 2301 1028 790 761 511 275 186 134 81 BsiHKAI G_wGCw'C Cuts at: 2307 4128 4213 5284 2307 Size: 1821 85 1071 3090 Fragments arranged by size: 3090 1821 1071 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 BsmBI CGTCTCn'nnnn_ Cuts at: 2669 2669 Size: 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2307 2463 3386 4128 4213 5284 Size: 399 318 199 156 923 742 85 1071 Cuts at: 5284 1391 Size: 2174 Fragments arranged by size: 2174 1071 923 742 399 318 199 156 85 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 3956 5686 3956 Size: 1730 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2400 3144 3422 844 Size: 1556 744 278 3489 Fragments arranged by size: 3489 1556 744 278 BsrBI CCG'CTC Cuts at: 1394 1823 2191 2640 2884 3954 1394 Size: 429 368 449 244 1070 3507 Fragments arranged by size: 3507 1070 449 429 368 244 BsrDI GCAATG_nn' Cuts at: 65 1248 2799 4562 4736 65 Size: 1183 1551 1763 174 1396 Fragments arranged by size: 1763 1551 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2094 2160 2763 3039 4711 4898 1700 Size: 110 284 66 603 276 1672 187 2869 Fragments arranged by size: 2869 1672 603 284 276 187 110 66 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6067 BssHII G'CGCG_C Cuts at: 1925 3207 3319 1925 Size: 1282 112 4673 Fragments arranged by size: 4673 1282 112 BssSI C'ACGA_G Cuts at: 3053 3314 4127 5139 3053 Size: 261 813 1012 3981 Fragments arranged by size: 3981 1012 813 261 BstAPI GCAn_nnn'nTGC Cuts at: 794 3157 794 Size: 2363 3704 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstXI CCAn_nnnn'nTGG Cuts at: 2743 2989 2743 Size: 246 5821 BstYI r'GATC_y Cuts at: 1972 2214 2370 2779 3397 3764 4149 4166 5618 Size: 242 156 409 618 367 385 17 1452 Cuts at: 5618 5704 5716 6062 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 618 409 385 367 346 242 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5515 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2392 2479 487 Size: 683 56 1166 87 4075 Fragments arranged by size: 4075 1166 683 87 56 BtsI GCAGTG_nn' Cuts at: 3583 4377 4397 3583 Size: 794 20 5253 Fragments arranged by size: 5253 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2068 2745 2869 Size: 54 1425 50 275 18 238 677 124 Cuts at: 2869 3003 3072 3273 3514 4403 6032 8 Size: 134 69 201 241 889 1629 43 Fragments arranged by size: 1629 1425 889 677 275 241 238 201 134 124 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2869 3003 3514 1537 Size: 275 1057 134 511 4090 Fragments arranged by size: 4090 1057 511 275 134 EarI CTCTTCn'nnn_ Cuts at: 1353 3997 1353 Size: 2644 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2336 2612 2860 4654 5028 5174 Size: 1064 138 747 276 248 1794 374 146 Cuts at: 5174 387 Size: 1280 Fragments arranged by size: 1794 1280 1064 747 374 276 248 146 138 EcoICRI GAG'CTC Cuts at: 2305 2305 Size: 6067 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2444 2612 2710 2915 2920 2930 Size: 393 671 141 168 98 205 5 10 Cuts at: 2930 3011 3226 3272 3347 4130 4727 5514 5782 Size: 81 215 46 75 783 597 787 268 Cuts at: 5782 1239 Size: 1524 Fragments arranged by size: 1524 787 783 671 597 393 268 215 205 168 141 98 81 75 46 10 5 EcoNI CCTnn'n_nnAGG Cuts at: 3128 3329 3128 Size: 201 5866 EcoO109I rG'GnC_Cy Cuts at: 3016 3133 3310 3016 Size: 117 177 5773 Fragments arranged by size: 5773 177 117 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2551 3256 2551 Size: 705 5362 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 2146 2893 2977 3071 3176 3238 3272 5214 Size: 468 747 84 94 105 62 34 1942 Cuts at: 5214 1678 Size: 2531 Fragments arranged by size: 2531 1942 747 468 105 94 84 62 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2890 2922 4708 Size: 32 135 32 743 32 543 32 1786 Cuts at: 4708 4740 4782 4814 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1786 743 543 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KasI G'GCGC_C Cuts at: 2889 2889 Size: 6067 KpnI G_GTAC'C Cuts at: 3106 3392 3106 Size: 286 5781 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 2903 5943 2903 Size: 3040 3027 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5181 5365 1305 Size: 3876 184 2007 Fragments arranged by size: 3876 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 3275 10 Size: 54 1425 343 915 528 2802 Fragments arranged by size: 2802 1425 915 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4025 4384 4543 518 Size: 648 2859 359 159 2042 Fragments arranged by size: 2859 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2067 2584 2868 3225 3483 4160 5308 5553 Size: 461 517 284 357 258 677 1148 245 Cuts at: 5553 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 517 461 357 284 258 245 NaeI GCC'GGC Cuts at: 2096 2162 2765 3041 2096 Size: 66 603 276 5122 Fragments arranged by size: 5122 603 276 66 NarI GG'CG_CC Cuts at: 2890 2890 Size: 6067 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5515 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NgoMIV G'CCGG_C Cuts at: 2094 2160 2763 3039 2094 Size: 66 603 276 5122 Fragments arranged by size: 5122 603 276 66 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 2869 3003 3514 2869 Size: 134 511 5422 Fragments arranged by size: 5422 511 134 NspI r_CATG'y Cuts at: 1132 1229 2605 3425 4970 1132 Size: 97 1376 820 1545 2229 Fragments arranged by size: 2229 1545 1376 820 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6067 PciI A'CATG_T Cuts at: 2601 4966 2601 Size: 2365 3702 PflMI CCAn_nnn'nTGG Cuts at: 1108 2812 1108 Size: 1704 4363 PfoI T'CCnGG_A Cuts at: 2280 2373 2822 2280 Size: 93 449 5525 Fragments arranged by size: 5525 449 93 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3133 3310 3133 Size: 177 5890 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PstI C_TGCA'G Cuts at: 830 3033 3345 3419 830 Size: 2203 312 74 3478 Fragments arranged by size: 3478 2203 312 74 PvuI CG_AT'CG Cuts at: 2001 2082 2001 Size: 81 5986 SacI G_AGCT'C Cuts at: 2307 2307 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 2723 4315 6027 2723 Size: 1592 1712 2763 Fragments arranged by size: 2763 1712 1592 SfcI C'TryA_G Cuts at: 826 1045 3029 3341 3415 4550 5231 5422 826 Size: 219 1984 312 74 1135 681 191 1471 Fragments arranged by size: 1984 1471 1135 681 312 219 191 74 SfoI GGC'GCC Cuts at: 2891 2891 Size: 6067 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2363 3161 3176 3230 4171 5072 Size: 197 926 420 798 15 54 941 901 Cuts at: 5072 5334 820 Size: 262 1553 Fragments arranged by size: 1553 941 926 901 798 420 262 197 54 15 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 3098 1517 Size: 1581 4486 StyI C'CwwG_G Cuts at: 513 1065 2748 2982 3201 3506 513 Size: 552 1683 234 219 305 3074 Fragments arranged by size: 3074 1683 552 305 234 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2369 3182 4094 4111 4264 4449 4894 Size: 1255 789 813 912 17 153 185 445 Cuts at: 4894 325 Size: 1498 Fragments arranged by size: 1498 1255 912 813 789 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2721 2916 4313 Size: 274 80 33 51 1443 744 195 1397 Cuts at: 4313 6025 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1397 744 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2599 2631 2839 2871 6027 6059 2599 Size: 32 208 32 3156 32 2607 Fragments arranged by size: 3156 2607 208 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2270 1316 Size: 954 5113 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 2522 2534 4196 2522 Size: 12 1662 4393 Fragments arranged by size: 4393 1662 12 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI