(Linear) (Six-base) MAPSORT of: KIBB6670.seq Check: 456 from: 1 to: 1362 KIBB6670.orf With 182 enzymes: SgfI * July 15, 2010 18:30 .. Acc65I G'GTAC_C Cuts at: 0 1097 1362 Size: 1097 265 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 705 910 925 1221 1362 Size: 439 266 205 15 296 141 Fragments arranged by size: 439 296 266 205 141 15 AfeI AGC'GCT Cuts at: 0 970 1362 Size: 970 392 AflIII A'CryG_T Cuts at: 0 596 898 1362 Size: 596 302 464 Fragments arranged by size: 596 464 302 AhdI GACnn_n'nnGTC Cuts at: 0 899 1362 Size: 899 463 AlwNI CAG_nnn'CTG Cuts at: 0 369 807 1131 1152 1362 Size: 369 438 324 21 210 Fragments arranged by size: 438 369 324 210 21 AvaI C'yCGr_G Cuts at: 0 874 1362 Size: 874 488 BanI G'GyrC_C Cuts at: 0 215 535 884 1097 1362 Size: 215 320 349 213 265 Fragments arranged by size: 349 320 265 215 213 BanII G_rGCy'C Cuts at: 0 302 1362 Size: 302 1060 BbeI G_GCGC'C Cuts at: 0 888 1362 Size: 888 474 BbsI GAAGACnn'nnnn_ Cuts at: 0 898 1362 Size: 898 464 BglI GCCn_nnn'nGGC Cuts at: 0 879 1362 Size: 879 483 Bme1580I G_kGCm'C Cuts at: 0 103 458 1362 Size: 103 355 904 Fragments arranged by size: 904 355 103 BmgBI CAC'GTC Cuts at: 0 24 264 1056 1362 Size: 24 240 792 306 Fragments arranged by size: 792 306 240 24 BmrI ACTGGGnnnn_n' Cuts at: 0 345 1362 Size: 345 1017 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 303 990 1362 Size: 303 687 372 Fragments arranged by size: 687 372 303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 607 915 1006 1267 1342 1362 Size: 298 309 308 91 261 75 20 Fragments arranged by size: 309 308 298 261 91 75 20 Bpu10I CC'TnA_GC Cuts at: 0 631 1362 Size: 631 731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 1141 1156 1210 1362 Size: 343 798 15 54 152 Fragments arranged by size: 798 343 152 54 15 BsaI GGTCTCn'nnnn_ Cuts at: 0 291 652 1362 Size: 291 361 710 Fragments arranged by size: 710 361 291 BsaHI Gr'CG_yC Cuts at: 0 885 1362 Size: 885 477 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 310 340 594 624 1081 1111 1362 Size: 310 30 254 30 457 30 251 Fragments arranged by size: 457 310 254 251 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 64 190 727 1003 1021 1096 1120 1204 Size: 64 126 537 276 18 75 24 84 Cuts at: 1204 1362 Size: 158 Fragments arranged by size: 537 276 158 126 84 75 64 24 18 BseYI C'CCAG_C Cuts at: 0 54 579 1209 1362 Size: 54 525 630 153 Fragments arranged by size: 630 525 153 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 373 1321 1362 Size: 220 153 948 41 Fragments arranged by size: 948 220 153 41 BsiEI CG_ry'CG Cuts at: 0 77 867 1001 1362 Size: 77 790 134 361 Fragments arranged by size: 790 361 134 77 BsiHKAI G_wGCw'C Cuts at: 0 302 1362 Size: 302 1060 BsmBI CGTCTCn'nnnn_ Cuts at: 0 664 1362 Size: 664 698 Bsp1286I G_dGCh'C Cuts at: 0 103 302 458 1362 Size: 103 199 156 904 Fragments arranged by size: 904 199 156 103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 395 1139 1362 Size: 395 744 223 Fragments arranged by size: 744 395 223 BsrBI CCG'CTC Cuts at: 0 186 635 879 1362 Size: 186 449 244 483 Fragments arranged by size: 483 449 244 186 BsrDI GCAATG_nn' Cuts at: 0 794 1362 Size: 794 568 BsrFI r'CCGG_y Cuts at: 0 89 155 758 1034 1362 Size: 89 66 603 276 328 Fragments arranged by size: 603 328 276 89 66 BssHII G'CGCG_C Cuts at: 0 1202 1314 1362 Size: 1202 112 48 Fragments arranged by size: 1202 112 48 BssSI C'ACGA_G Cuts at: 0 1048 1309 1362 Size: 1048 261 53 Fragments arranged by size: 1048 261 53 BstAPI GCAn_nnn'nTGC Cuts at: 0 1152 1362 Size: 1152 210 BstXI CCAn_nnnn'nTGG Cuts at: 0 738 984 1362 Size: 738 246 378 Fragments arranged by size: 738 378 246 BstYI r'GATC_y Cuts at: 0 209 365 774 1362 Size: 209 156 409 588 Fragments arranged by size: 588 409 209 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 387 474 1362 Size: 387 87 888 Fragments arranged by size: 888 387 87 EaeI y'GGCC_r Cuts at: 0 63 740 864 998 1067 1268 1362 Size: 63 677 124 134 69 201 94 Fragments arranged by size: 677 201 134 124 94 69 63 EagI C'GGCC_G Cuts at: 0 864 998 1362 Size: 864 134 364 Fragments arranged by size: 864 364 134 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 331 607 855 1362 Size: 331 276 248 507 Fragments arranged by size: 507 331 276 248 EcoICRI GAG'CTC Cuts at: 0 300 1362 Size: 300 1062 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 439 607 705 910 915 925 1006 Size: 298 141 168 98 205 5 10 81 Cuts at: 1006 1221 1267 1342 1362 Size: 215 46 75 20 Fragments arranged by size: 298 215 205 168 141 98 81 75 46 20 10 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 1123 1324 1362 Size: 1123 201 38 Fragments arranged by size: 1123 201 38 EcoO109I rG'GnC_Cy Cuts at: 0 1011 1128 1305 1362 Size: 1011 117 177 57 Fragments arranged by size: 1011 177 117 57 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 546 1251 1362 Size: 546 705 111 Fragments arranged by size: 705 546 111 HaeII r_GCGC'y Cuts at: 0 141 888 972 1066 1171 1233 1267 1362 Size: 141 747 84 94 105 62 34 95 Fragments arranged by size: 747 141 105 95 94 84 62 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 310 342 885 917 1362 Size: 310 32 543 32 445 Fragments arranged by size: 543 445 310 32 32 KasI G'GCGC_C Cuts at: 0 884 1362 Size: 884 478 KpnI G_GTAC'C Cuts at: 0 1101 1362 Size: 1101 261 MluI A'CGCG_T Cuts at: 0 898 1362 Size: 898 464 MscI TGG'CCA Cuts at: 0 742 1270 1362 Size: 742 528 92 Fragments arranged by size: 742 528 92 MspA1I CmG'CkG Cuts at: 0 62 579 863 1220 1362 Size: 62 517 284 357 142 Fragments arranged by size: 517 357 284 142 62 NaeI GCC'GGC Cuts at: 0 91 157 760 1036 1362 Size: 91 66 603 276 326 Fragments arranged by size: 603 326 276 91 66 NarI GG'CG_CC Cuts at: 0 885 1362 Size: 885 477 NgoMIV G'CCGG_C Cuts at: 0 89 155 758 1034 1362 Size: 89 66 603 276 328 Fragments arranged by size: 603 328 276 89 66 NotI GC'GGCC_GC Cuts at: 0 864 998 1362 Size: 864 134 364 Fragments arranged by size: 864 364 134 NspI r_CATG'y Cuts at: 0 600 1362 Size: 600 762 PciI A'CATG_T Cuts at: 0 596 1362 Size: 596 766 PflMI CCAn_nnn'nTGG Cuts at: 0 807 1362 Size: 807 555 PfoI T'CCnGG_A Cuts at: 0 275 368 817 1362 Size: 275 93 449 545 Fragments arranged by size: 545 449 275 93 PpuMI rG'GwC_Cy Cuts at: 0 1128 1305 1362 Size: 1128 177 57 Fragments arranged by size: 1128 177 57 PstI C_TGCA'G Cuts at: 0 1028 1340 1362 Size: 1028 312 22 Fragments arranged by size: 1028 312 22 PvuI CG_AT'CG Cuts at: 0 77 1362 Size: 77 1285 SacI G_AGCT'C Cuts at: 0 302 1362 Size: 302 1060 ScaI AGT'ACT Cuts at: 0 718 1362 Size: 718 644 SfcI C'TryA_G Cuts at: 0 1024 1336 1362 Size: 1024 312 26 Fragments arranged by size: 1024 312 26 SfoI GGC'GCC Cuts at: 0 886 1362 Size: 886 476 SmlI C'TyrA_G Cuts at: 0 358 1156 1171 1225 1362 Size: 358 798 15 54 137 Fragments arranged by size: 798 358 137 54 15 StuI AGG'CCT Cuts at: 0 1093 1362 Size: 1093 269 StyI C'CwwG_G Cuts at: 0 743 977 1196 1362 Size: 743 234 219 166 Fragments arranged by size: 743 234 219 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 364 1177 1362 Size: 364 813 185 Fragments arranged by size: 813 364 185 TatI w'GTAC_w Cuts at: 0 716 911 1362 Size: 716 195 451 Fragments arranged by size: 716 451 195 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 594 626 834 866 1362 Size: 594 32 208 32 496 Fragments arranged by size: 594 496 208 32 32 Tth111I GACn'n_nGTC Cuts at: 0 265 1362 Size: 265 1097 XmnI GAAnn'nnTTC Cuts at: 0 517 529 1362 Size: 517 12 833 Fragments arranged by size: 833 517 12 Enzymes that do cut: Acc65I AcuI AfeI AflIII AhdI AlwNI AvaI BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgZI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI MluI MscI MspA1I NaeI NarI NgoMIV NotI NspI PciI PflMI PfoI PpuMI PstI PvuI SacI ScaI SfcI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrGI BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI MmeI MslI NcoI NdeI NheI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuII RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI ZraI