Restriction map for
pFN21AB0459
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AviII
AvrII BamHI BbvCI BcgI BciVI
BclI BfrI BlnI BsaBI BsaWI
BseAI BsiWI BsmI BspEI BspLU11I
BsrDI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CpoI DraI DrdI Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KspI MamI MfeI MluI MroI
MseI MunI NcoI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI TfiI Tru9I Tsp509I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 496 GACGTC GACGT^C
AlwI 885 GATCC GGATC(4/5)
ApaI 143 GGGCCC GGGCC^C
BbeI 1176 GGCGCC GGCGC^C
BbsI 1252 GTCTTC GAAGAC(2/6)
BfaI 1144 CTAG C^TAG
BglII 380 AGATCT A^GATCT
BpuAI 1252 GTCTTC GAAGAC(2/6)
Bsp1407I 902 TGTACA T^GTACA
BspDI 541 ATCGAT AT^CGAT
BsrGI 902 TGTACA T^GTACA
BssHII 451 GCGCGC G^CGCGC
BstEII 225 GGTCACC G^GT[ACGT]ACC
BstPI 225 GGTCACC G^GT[ACGT]ACC
BstXI 276 CCACACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 380 AGATCT [GA]^GATC[CT]
ClaI 541 ATCGAT AT^CGAT
DraIII 153 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 26 GAAGAG CTCTTC(1/4)
EcoO65I 225 GGTCACC G^GT[ACGT]ACC
EcoT14I 1100 CCAAGG C^C[AT][AT]GG
HindIII 1042 AAGCTT A^AGCTT
KasI 1176 GGCGCC G^GCGCC
Ksp632I 26 GAAGAG CTCTTC(1/4)
MaeI 1144 CTAG C^TAG
MflI 380 AGATCT [GA]^GATC[CT]
NarI 1176 GGCGCC GG^CGCC
NdeI 1259 CATATG CA^TATG
PshAI 831 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 750 CCTGCAGG CCTGCA^GG
SfoI 1176 GGCGCC GGC^GCC
SmlI 1149 CTCAAG C^T[CT][GA]AG
Sse8387I 750 CCTGCAGG CCTGCA^GG
SspBI 902 TGTACA T^GTACA
StyI 1100 CCAAGG C^C[AT][AT]GG
XcmI 130 CCAACGAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 380 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 38 enzymes) #
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Acc65I 260 GGTACC G^GTACC
1322 GGTACC
AhdI 51 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACCCGGGGTC
Alw44I 1048 GTGCAC G^TGCAC
1360 GTGCAC
ApaLI 1048 GTGCAC G^TGCAC
1360 GTGCAC
Asp718I 260 GGTACC G^GTACC
1322 GGTACC
AspEI 51 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACCCGGGGTC
BbrPI 919 CACGTG CAC^GTG
1006 CACGTG
BglI 129 GCCAACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
455 GCCACCCCGGC
BlpI 351 GCTCAGC GC^T[ACGT]AGC
982 GCTCAGC
Bpu1102I 351 GCTCAGC GC^T[ACGT]AGC
982 GCTCAGC
BsaAI 919 CACGTG [CT]AC^GT[GA]
1006 CACGTG
BsaI 627 GAGACC GGTCTC(1/5)
1080 GGTCTC
BseRI 661 GAGGAG GAGGAG(10/8)
664 GAGGAG
BsiEI 656 CGGCCG CG[GA][CT]^CG
833 CGGCCG
BspHI 839 TCATGA T^CATGA
1370 TCATGA
BspMI 695 ACCTGC ACCTGC(4/8)
1456 GCAGGT
BsrFI 1154 GCCGGC [GA]^CCGG[CT]
1403 GCCGGC
BssSI 856 CACGAG CACGAG(-5/-1)
970 CTCGTG
CelII 351 GCTCAGC GC^T[ACGT]AGC
982 GCTCAGC
Cfr10I 1154 GCCGGC [GA]^CCGG[CT]
1403 GCCGGC
EagI 656 CGGCCG C^GGCCG
833 CGGCCG
Eam1105I 51 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACCCGGGGTC
EclXI 656 CGGCCG C^GGCCG
833 CGGCCG
Eco52I 656 CGGCCG C^GGCCG
833 CGGCCG
EcoNI 40 CCTCGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
888 CCTGCACCAGG
HinfI 445 GAGTC G^A[ACGT]TC
1147 GACTC
KpnI 260 GGTACC GGTAC^C
1322 GGTACC
NaeI 1154 GCCGGC GCC^GGC
1403 GCCGGC
NgoMIV 1154 GCCGGC G^CCGGC
1403 GCCGGC
PleI 1147 GACTC GAGTC(4/5)
445 GAGTC
PmaCI 919 CACGTG CAC^GTG
1006 CACGTG
PmlI 919 CACGTG CAC^GTG
1006 CACGTG
PpuMI 49 GGGACCC [GA]G^G[AT]CC[CT]
1088 AGGACCT
RcaI 839 TCATGA T^CATGA
1370 TCATGA
SfaNI 126 GATGC GCATC(5/9)
849 GATGC
StuI 239 AGGCCT AGG^CCT
815 AGGCCT
TaqI 542 TCGA T^CGA
963 TCGA
TthHB8I 542 TCGA T^CGA
963 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AspI AvaI AvaII
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflFI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfcI SmaI TseI
Tsp45I TspRI Tth111I XmaCI XmaI