Restriction map for
pFN21AB0459
(Linear) (Six-base) MAPSORT of: KIBB0459.seq Check: 9041 from: 1 to: 1524
KIBB0459.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1447 1524
Size: 1447 77
AatII G_ACGT'C
Cuts at: 0 500 1524
Size: 500 1024
Acc65I G'GTAC_C
Cuts at: 0 260 1322 1524
Size: 260 1062 202
Fragments arranged by size:
1062 260 202
AhdI GACnn_n'nnGTC
Cuts at: 0 56 959 1524
Size: 56 903 565
Fragments arranged by size:
903 565 56
AleI CACnn'nnGTG
Cuts at: 0 281 1524
Size: 281 1243
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 415 447 1524
Size: 415 32 1077
Fragments arranged by size:
1077 415 32
AlwNI CAG_nnn'CTG
Cuts at: 0 72 408 900 990 1382 1524
Size: 72 336 492 90 392 142
Fragments arranged by size:
492 392 336 142 90 72
ApaI G_GGCC'C
Cuts at: 0 147 1524
Size: 147 1377
ApaLI G'TGCA_C
Cuts at: 0 1048 1360 1524
Size: 1048 312 164
Fragments arranged by size:
1048 312 164
AvaI C'yCGr_G
Cuts at: 0 147 384 418 956 1180 1339 1439 1524
Size: 147 237 34 538 224 159 100 85
Fragments arranged by size:
538 237 224 159 147 100 85 34
BanI G'GyrC_C
Cuts at: 0 101 151 260 1176 1322 1459 1524
Size: 101 50 109 916 146 137 65
Fragments arranged by size:
916 146 137 109 101 65 50
BanII G_rGCy'C
Cuts at: 0 82 147 969 1272 1397 1523 1524
Size: 82 65 822 303 125 126 1
Fragments arranged by size:
822 303 126 125 82 65 1
BbeI G_GCGC'C
Cuts at: 0 1180 1524
Size: 1180 344
BbsI GAAGACnn'nnnn_
Cuts at: 0 1245 1524
Size: 1245 279
BglI GCCn_nnn'nGGC
Cuts at: 0 135 461 1524
Size: 135 326 1063
Fragments arranged by size:
1063 326 135
BglII A'GATC_T
Cuts at: 0 380 1524
Size: 380 1144
BlpI GC'TnA_GC
Cuts at: 0 352 983 1524
Size: 352 631 541
Fragments arranged by size:
631 541 352
Bme1580I G_kGCm'C
Cuts at: 0 147 154 1052 1364 1464 1524
Size: 147 7 898 312 100 60
Fragments arranged by size:
898 312 147 100 60 7
BmgBI CAC'GTC
Cuts at: 0 222 1365 1524
Size: 222 1143 159
Fragments arranged by size:
1143 222 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 150 1524
Size: 150 1374
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 351 1272 1524
Size: 351 921 252
Fragments arranged by size:
921 351 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1051 1245 1524
Size: 256 795 194 279
Fragments arranged by size:
795 279 256 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 1524
Size: 1134 390
BsaI GGTCTCn'nnnn_
Cuts at: 0 621 1086 1524
Size: 621 465 438
Fragments arranged by size:
621 465 438
BsaAI yAC'GTr
Cuts at: 0 921 1008 1524
Size: 921 87 516
Fragments arranged by size:
921 516 87
BsaHI Gr'CG_yC
Cuts at: 0 497 1177 1487 1524
Size: 497 680 310 37
Fragments arranged by size:
680 497 310 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1306 1336 1524
Size: 1306 30 188
Fragments arranged by size:
1306 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 676 679 1524
Size: 676 3 845
Fragments arranged by size:
845 676 3
BseYI C'CCAG_C
Cuts at: 0 140 307 1382 1464 1524
Size: 140 167 1075 82 60
Fragments arranged by size:
1075 167 140 82 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 597 874 988 1475 1524
Size: 502 95 277 114 487 49
Fragments arranged by size:
502 487 277 114 95 49
BsiEI CG_ry'CG
Cuts at: 0 659 836 1524
Size: 659 177 688
Fragments arranged by size:
688 659 177
BsiHKAI G_wGCw'C
Cuts at: 0 179 720 920 1052 1364 1524
Size: 179 541 200 132 312 160
Fragments arranged by size:
541 312 200 179 160 132
BsmBI CGTCTCn'nnnn_
Cuts at: 0 120 823 877 1479 1524
Size: 120 703 54 602 45
Fragments arranged by size:
703 602 120 54 45
Bsp1286I G_dGCh'C
Cuts at: 0 82 147 154 179 720 920 969 1052
Size: 82 65 7 25 541 200 49 83
Cuts at: 1052 1272 1364 1397 1464 1523 1524
Size: 220 92 33 67 59 1
Fragments arranged by size:
541 220 200 92 83 82 67 65
59 49 33 25 7 1
BspHI T'CATG_A
Cuts at: 0 839 1370 1524
Size: 839 531 154
Fragments arranged by size:
839 531 154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 704 1447 1524
Size: 704 743 77
Fragments arranged by size:
743 704 77
BsrBI CCG'CTC
Cuts at: 0 324 982 1134 1524
Size: 324 658 152 390
Fragments arranged by size:
658 390 324 152
BsrFI r'CCGG_y
Cuts at: 0 1154 1403 1524
Size: 1154 249 121
Fragments arranged by size:
1154 249 121
BsrGI T'GTAC_A
Cuts at: 0 902 1524
Size: 902 622
BssHII G'CGCG_C
Cuts at: 0 451 1524
Size: 451 1073
BssSI C'ACGA_G
Cuts at: 0 856 970 1524
Size: 856 114 554
Fragments arranged by size:
856 554 114
BstEII G'GTnAC_C
Cuts at: 0 225 1524
Size: 225 1299
BstXI CCAn_nnnn'nTGG
Cuts at: 0 283 1524
Size: 283 1241
BstYI r'GATC_y
Cuts at: 0 380 1524
Size: 380 1144
BtgI C'CryG_G
Cuts at: 0 54 364 372 1310 1524
Size: 54 310 8 938 214
Fragments arranged by size:
938 310 214 54 8
BtsI GCAGTG_nn'
Cuts at: 0 1458 1524
Size: 1458 66
ClaI AT'CG_AT
Cuts at: 0 542 1524
Size: 542 982
DraIII CAC_nnn'GTG
Cuts at: 0 158 1524
Size: 158 1366
EaeI y'GGCC_r
Cuts at: 0 489 528 656 671 779 833 1010 1061
Size: 489 39 128 15 108 54 177 51
Cuts at: 1061 1097 1121 1405 1424 1524
Size: 36 24 284 19 100
Fragments arranged by size:
489 284 177 128 108 100 54 51
39 36 24 19 15
EagI C'GGCC_G
Cuts at: 0 656 833 1524
Size: 656 177 691
Fragments arranged by size:
691 656 177
EarI CTCTTCn'nnn_
Cuts at: 0 21 1524
Size: 21 1503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1051 1245 1524
Size: 256 795 194 279
Fragments arranged by size:
795 279 256 194
EcoNI CCTnn'n_nnAGG
Cuts at: 0 44 892 1524
Size: 44 848 632
Fragments arranged by size:
848 632 44
EcoO109I rG'GnC_Cy
Cuts at: 0 50 144 330 1089 1354 1524
Size: 50 94 186 759 265 170
Fragments arranged by size:
759 265 186 170 94 50
HaeII r_GCGC'y
Cuts at: 0 141 316 608 1180 1524
Size: 141 175 292 572 344
Fragments arranged by size:
572 344 292 175 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 707 739 945 977 1524
Size: 42 32 633 32 206 32 547
Fragments arranged by size:
633 547 206 42 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1042 1524
Size: 1042 482
KasI G'GCGC_C
Cuts at: 0 1176 1524
Size: 1176 348
KpnI G_GTAC'C
Cuts at: 0 264 1326 1524
Size: 264 1062 198
Fragments arranged by size:
1062 264 198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1283 1524
Size: 1196 87 241
Fragments arranged by size:
1196 241 87
MscI TGG'CCA
Cuts at: 0 673 1099 1123 1524
Size: 673 426 24 401
Fragments arranged by size:
673 426 401 24
MslI CAynn'nnrTG
Cuts at: 0 281 574 910 1524
Size: 281 293 336 614
Fragments arranged by size:
614 336 293 281
MspA1I CmG'CkG
Cuts at: 0 307 408 1379 1524
Size: 307 101 971 145
Fragments arranged by size:
971 307 145 101
NaeI GCC'GGC
Cuts at: 0 1156 1405 1524
Size: 1156 249 119
Fragments arranged by size:
1156 249 119
NarI GG'CG_CC
Cuts at: 0 1177 1524
Size: 1177 347
NdeI CA'TA_TG
Cuts at: 0 1260 1524
Size: 1260 264
NgoMIV G'CCGG_C
Cuts at: 0 1154 1403 1524
Size: 1154 249 121
Fragments arranged by size:
1154 249 121
PfoI T'CCnGG_A
Cuts at: 0 233 821 1524
Size: 233 588 703
Fragments arranged by size:
703 588 233
PmlI CAC'GTG
Cuts at: 0 921 1008 1524
Size: 921 87 516
Fragments arranged by size:
921 516 87
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 1524
Size: 538 32 954
Fragments arranged by size:
954 538 32
PpuMI rG'GwC_Cy
Cuts at: 0 50 1089 1524
Size: 50 1039 435
Fragments arranged by size:
1039 435 50
PshAI GACnn'nnGTC
Cuts at: 0 835 1524
Size: 835 689
PspOMI G'GGCC_C
Cuts at: 0 143 1524
Size: 143 1381
PstI C_TGCA'G
Cuts at: 0 413 701 755 995 1524
Size: 413 288 54 240 529
Fragments arranged by size:
529 413 288 240 54
PvuII CAG'CTG
Cuts at: 0 307 408 1379 1524
Size: 307 101 971 145
Fragments arranged by size:
971 307 145 101
SanDI GG'GwC_CC
Cuts at: 0 50 1524
Size: 50 1474
SbfI CC_TGCA'GG
Cuts at: 0 755 1524
Size: 755 769
SexAI A'CCwGG_T
Cuts at: 0 863 935 1091 1524
Size: 863 72 156 433
Fragments arranged by size:
863 433 156 72
SfcI C'TryA_G
Cuts at: 0 409 697 751 991 1524
Size: 409 288 54 240 533
Fragments arranged by size:
533 409 288 240 54
SfoI GGC'GCC
Cuts at: 0 1178 1524
Size: 1178 346
SmaI CCC'GGG
Cuts at: 0 149 958 1441 1524
Size: 149 809 483 83
Fragments arranged by size:
809 483 149 83
SmlI C'TyrA_G
Cuts at: 0 1149 1524
Size: 1149 375
StuI AGG'CCT
Cuts at: 0 241 817 1524
Size: 241 576 707
Fragments arranged by size:
707 576 241
StyI C'CwwG_G
Cuts at: 0 1100 1524
Size: 1100 424
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 169 1524
Size: 169 1355
TatI w'GTAC_w
Cuts at: 0 902 1524
Size: 902 622
Tth111I GACn'n_nGTC
Cuts at: 0 223 469 651 1524
Size: 223 246 182 873
Fragments arranged by size:
873 246 223 182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 137 1524
Size: 137 1387
XmaI C'CCGG_G
Cuts at: 0 147 956 1439 1524
Size: 147 809 483 85
Fragments arranged by size:
809 483 147 85
ZraI GAC'GTC
Cuts at: 0 498 1524
Size: 498 1026
Enzymes that do cut:
AarI AatII Acc65I AhdI AleI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstEII BstXI BstYI BtgI BtsI ClaI DraIII
EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV PfoI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuII SanDI SbfI SexAI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI
XmaI ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AflIII AgeI AlfI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BmtI Bpu10I BsaBI BsaWI
BsiWI BsmI BspEI BsrDI BstAPI BstBI BstZ17I Bsu36I
BtgZI CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI MfeI MluI
NcoI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XhoI XmnI