################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI DpnII DraI DraIII DrdI Eam1105I EarI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MseI MslI MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 208 CGCG CG^CG AfaI 162 GTAC GT^AC Alw44I 190 GTGCAC G^TGCAC AocI 199 CCTGAGG CC^T[ACGT]AGG Aor51HI 312 AGCGCT AGC^GCT ApaLI 190 GTGCAC G^TGCAC AspHI 190 GTGCAC G[AT]GC[AT]^C AvaII 373 GGACC G^G[AT]CC BalI 506 TGGCCA TGG^CCA BbeI 245 GGCGCC GGCGC^C BglI 401 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 80 ACCGGA [AT]^CCGG[AT] BsiEI 380 CGGCCG CG[GA][CT]^CG BsiHKAI 190 GTGCAC G[AT]GC[AT]^C BsmAI 294 GAGAC GTCTC(1/5) BsmFI 492 GTCCC GGGAC(10/14) Bsp1407I 161 TGTACA T^GTACA BspMI 213 GCAGGT ACCTGC(4/8) BsrGI 161 TGTACA T^GTACA BstF5I 156 GGATG GGATG(2/0) BstUI 208 CGCG CG^CG Bsu36I 199 CCTGAGG CC^T[ACGT]AGG CpoI 372 CGGACCG CG^G[AT]CCG DsaI 281 CCGTGG C^C[GA][CT]GG EagI 380 CGGCCG C^GGCCG EclXI 380 CGGCCG C^GGCCG Eco47III 312 AGCGCT AGC^GCT Eco52I 380 CGGCCG C^GGCCG Eco81I 199 CCTGAGG CC^T[ACGT]AGG FokI 156 GGATG GGATG(9/13) HphI 101 TCACC GGTGA(8/7) KasI 245 GGCGCC G^GCGCC MboII 72 GAAGA GAAGA(8/7) MluNI 506 TGGCCA TGG^CCA MscI 506 TGGCCA TGG^CCA MvnI 208 CGCG CG^CG NarI 245 GGCGCC GG^CGCC NspI 516 ACATGC [GA]CATG^[CT] RsaI 162 GTAC GT^AC RsrII 372 CGGACCG CG^G[AT]CCG SfoI 245 GGCGCC GGC^GCC SspBI 161 TGTACA T^GTACA TaqI 137 TCGA T^CGA TthHB8I 137 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 245 GGCGCC G[GA]^CG[CT]C 489 GGCGTC AlwNI 523 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 534 CAGGGCCTG BanI 245 GGCGCC G^G[CT][GA]CC 277 GGTGCC BsaHI 245 GGCGCC G[GA]^CG[CT]C 489 GGCGTC BsgI 115 CTGCAC GTGCAG(16/14) 211 GTGCAG BsrBI 127 GAGCGG CCGCTC(-3/-3) 256 GAGCGG BsrFI 24 GCCGGC [GA]^CCGG[CT] 378 GCCGGC Cfr10I 24 GCCGGC [GA]^CCGG[CT] 378 GCCGGC DdeI 7 CTGAG C^T[ACGT]AG 200 CTGAG EcoNI 195 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 480 CCTGGAGGAGG EcoT14I 185 CCAAGG C^C[AT][AT]GG 509 CCAAGG HaeII 245 GGCGCC [GA]GCGC^[CT] 312 AGCGCT HgaI 430 GCGTC GACGC(5/10) 490 GCGTC Hin1I 245 GGCGCC G[GA]^CG[CT]C 489 GGCGTC HinfI 265 GAGTC G^A[ACGT]TC 296 GACTC MspA1I 112 CAGCTG C[AC]G^C[GT]G 121 CAGCTG NaeI 24 GCCGGC GCC^GGC 378 GCCGGC NgoMIV 24 GCCGGC G^CCGGC 378 GCCGGC PleI 296 GACTC GAGTC(4/5) 265 GAGTC PvuII 112 CAGCTG CAG^CTG 121 CAGCTG StuI 140 AGGCCT AGG^CCT 390 AGGCCT StyI 185 CCAAGG C^C[AT][AT]GG 509 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI ApaI AvaI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsoBI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SmaI TseI XmaCI XmaI