Restriction map for
pFN21AB8883
(Circular) (Six-base) MAPSORT of: pFN21AB8883.seq Check: 385 from: 1 to: 5257
pFN21AB8883 5257 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2209 2209
Size: 5257
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3067 278
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2600 1937
Size: 663 4594
Acc65I G'GTAC_C
Cuts at: 2578 2578
Size: 5257
AclI AA'CG_TT
Cuts at: 1563 3384 3757 1563
Size: 1821 373 3063
Fragments arranged by size:
3063 1821 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3320 4704 4972 1632
Size: 1688 1384 268 1917
Fragments arranged by size:
1917 1688 1384 268
AfeI AGC'GCT
Cuts at: 2319 2319
Size: 5257
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5060
AflIII A'CryG_T
Cuts at: 1203 4156 5133 1203
Size: 2953 977 1327
Fragments arranged by size:
2953 1327 977
AgeI A'CCGG_T
Cuts at: 4088 4088
Size: 5257
AhdI GACnn_n'nnGTC
Cuts at: 3986 3986
Size: 5257
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 2123
Size: 32 5225
AlwNI CAG_nnn'CTG
Cuts at: 2533 2544 3000 4572 2533
Size: 11 456 1572 3218
Fragments arranged by size:
3218 1572 456 11
ApaI G_GGCC'C
Cuts at: 2375 2417 2423 2375
Size: 42 6 5209
Fragments arranged by size:
5209 42 6
ApaLI G'TGCA_C
Cuts at: 2195 3314 4470 2195
Size: 1119 1156 2982
Fragments arranged by size:
2982 1156 1119
ApoI r'AATT_y
Cuts at: 1493 2566 2792 2944 5053 1493
Size: 1073 226 152 2109 1697
Fragments arranged by size:
2109 1697 1073 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3811 160
Size: 872 2779 1606
Fragments arranged by size:
2779 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2278 2306 2416 2422 2582 1870
Size: 73 74 261 28 110 6 160 4545
Fragments arranged by size:
4545 261 160 110 74 73 28 6
BamHI G'GATC_C
Cuts at: 2587 2954 2587
Size: 367 4890
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2282 2578 4033 618
Size: 325 217 627 463 32 296 1455 1842
Fragments arranged by size:
1842 1455 627 463 325 296 217 32
BanII G_rGCy'C
Cuts at: 1391 2018 2364 2375 2417 2423 2576 1391
Size: 627 346 11 42 6 153 4072
Fragments arranged by size:
4072 627 346 153 42 11 6
BbeI G_GCGC'C
Cuts at: 2254 2254
Size: 5257
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3448 3482 1903
Size: 34 1511 34 3678
Fragments arranged by size:
3678 1511 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3149 4359 3149
Size: 1210 4047
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5257
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2412 3868 136
Size: 107 122 71 631 605 740 1456 1525
Fragments arranged by size:
1525 1456 740 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5252 5252
Size: 5257
BlpI GC'TnA_GC
Cuts at: 2674 2674
Size: 5257
Bme1580I G_kGCm'C
Cuts at: 1790 2199 2375 2417 2423 3318 4474 1790
Size: 409 176 42 6 895 1156 2573
Fragments arranged by size:
2573 1156 895 409 176 42 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5257
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2988 3946 442
Size: 416 53 83 399 24 1571 958 1753
Fragments arranged by size:
1753 1571 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 5257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2288 2378 2507 3917 1239
Size: 911 138 90 129 1410 2579
Fragments arranged by size:
2579 1410 911 138 129 90
Bpu10I CC'TnA_GC
Cuts at: 2012 2012
Size: 5257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3382 4247 4545 3382
Size: 865 298 4094
Fragments arranged by size:
4094 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3920 882
Size: 617 2421 2219
Fragments arranged by size:
2421 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3048 493
Size: 711 1844 2702
Fragments arranged by size:
2702 1844 711
BsaBI GATnn'nnATC
Cuts at: 5251 5251
Size: 5257
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2251 2495 3064 3446
Size: 53 83 186 936 718 244 569 382
Cuts at: 3446 4081 275
Size: 635 1451
Fragments arranged by size:
1451 936 718 635 569 382 244 186
83 53
BsaWI w'CCGG_w
Cuts at: 2085 3690 4088 4362 4509 4920 2085
Size: 1605 398 274 147 411 2422
Fragments arranged by size:
2422 1605 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 2351 2381 2480
Size: 30 165 30 720 30 198 30 99
Cuts at: 2480 2510 1178
Size: 30 3925
Fragments arranged by size:
3925 720 198 165 99 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2192 2504 1186
Size: 892 114 312 3939
Fragments arranged by size:
3939 892 312 114
BseYI C'CCAG_C
Cuts at: 2314 4460 4956 2314
Size: 2146 496 2615
Fragments arranged by size:
2615 2146 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2105 2237 1163
Size: 573 369 132 4183
Fragments arranged by size:
4183 573 369 132
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2388 2707 3468 4496 1540
Size: 275 186 387 319 761 1028 2301
Fragments arranged by size:
2301 1028 761 387 319 275 186
BsiHKAI G_wGCw'C
Cuts at: 2199 3318 3403 4474 2199
Size: 1119 85 1071 2982
Fragments arranged by size:
2982 1119 1071 85
BsmI GAATG_Cn'
Cuts at: 2768 2861 2768
Size: 93 5164
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2199 2364 2375 2417 2423 2576
Size: 399 228 181 165 11 42 6 153
Cuts at: 2576 3318 3403 4474 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 228 181 165 153
85 42 11 6
BspEI T'CCGG_A
Cuts at: 4920 4920
Size: 5257
BspHI T'CATG_A
Cuts at: 3146 4876 3146
Size: 1730 3527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2209 2612 844
Size: 1365 403 3489
Fragments arranged by size:
3489 1365 403
BsrBI CCG'CTC
Cuts at: 1394 1823 2134 2263 3144 1394
Size: 429 311 129 881 3507
Fragments arranged by size:
3507 881 429 311 129
BsrDI GCAATG_nn'
Cuts at: 65 1248 3752 3926 65
Size: 1183 2504 174 1396
Fragments arranged by size:
2504 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 2383 3901 4088 1700
Size: 110 219 354 1518 187 2869
Fragments arranged by size:
2869 1518 354 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 2166 96
Size: 2070 3187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5257
BssSI C'ACGA_G
Cuts at: 3317 4329 3317
Size: 1012 4245
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5257
BstBI TT'CG_AA
Cuts at: 4061 4061
Size: 5257
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5257
BstYI r'GATC_y
Cuts at: 1972 2587 2954 3339 3356 4808 4894 4906 5252
Size: 615 367 385 17 1452 86 12 346
Cuts at: 5252 1972
Size: 1977
Fragments arranged by size:
1977 1452 615 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2205 2205
Size: 5257
BtgI C'CryG_G
Cuts at: 513 1065 2286 513
Size: 552 1221 3484
Fragments arranged by size:
3484 1221 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4518
Fragments arranged by size:
4518 683 56
BtsI GCAGTG_nn'
Cuts at: 2773 3567 3587 2773
Size: 794 20 4443
Fragments arranged by size:
4443 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5222
DraI TTT'AAA
Cuts at: 2561 2561
Size: 5257
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4264 809
Size: 507 2948 1802
Fragments arranged by size:
2948 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2340 2385 2511
Size: 54 1425 50 275 18 510 45 126
Cuts at: 2511 2546 2704 3593 5222 8
Size: 35 158 889 1629 43
Fragments arranged by size:
1629 1425 889 510 275 158 126 54
50 45 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2385 2704 1537
Size: 275 573 319 4090
Fragments arranged by size:
4090 573 319 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3187 1353
Size: 1834 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3844 4218 4364 387
Size: 1064 138 2255 374 146 1280
Fragments arranged by size:
2255 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2288 2378 2507 3320 3917 4704
Size: 393 518 138 90 129 813 597 787
Cuts at: 4704 4972 1239
Size: 268 1524
Fragments arranged by size:
1524 813 787 597 518 393 268 138
129 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2204 2489 2204
Size: 285 4972
EcoO109I rG'GnC_Cy
Cuts at: 2068 2311 2372 2541 2068
Size: 243 61 169 4784
Fragments arranged by size:
4784 243 169 61
EcoRI G'AATT_C
Cuts at: 2566 2944 2566
Size: 378 4879
FspI TGC'GCA
Cuts at: 3763 3763
Size: 5257
HaeII r_GCGC'y
Cuts at: 1678 2254 2321 4404 1678
Size: 576 67 2083 2531
Fragments arranged by size:
2531 2083 576 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2480 2512 3898 3930 3972
Size: 32 135 32 908 32 1386 32 42
Cuts at: 3972 4004 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 908 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2601 2847 669
Size: 988 281 663 246 3079
Fragments arranged by size:
3079 988 663 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5257
HpaI GTT'AAC
Cuts at: 2847 2847
Size: 5257
KasI G'GCGC_C
Cuts at: 2250 2250
Size: 5257
KpnI G_GTAC'C
Cuts at: 2582 2582
Size: 5257
MfeI C'AATT_G
Cuts at: 2856 2856
Size: 5257
MluI A'CGCG_T
Cuts at: 5133 5133
Size: 5257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4371 4555 1305
Size: 3066 184 2007
Fragments arranged by size:
3066 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 10
Size: 54 1425 343 681 2754
Fragments arranged by size:
2754 1425 681 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3215 3574 3733 518
Size: 648 2049 359 159 2042
Fragments arranged by size:
2049 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2128 2673 3350 4498 4743 1606
Size: 513 9 545 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 545 513 245 9
NaeI GCC'GGC
Cuts at: 2031 2385 2031
Size: 354 4903
NarI GG'CG_CC
Cuts at: 2251 2251
Size: 5257
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4705
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5257
NgoMIV G'CCGG_C
Cuts at: 2029 2383 2029
Size: 354 4903
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5257
NotI GC'GGCC_GC
Cuts at: 2704 2704
Size: 5257
NspI r_CATG'y
Cuts at: 1132 1229 2525 2615 4160 1132
Size: 97 1296 90 1545 2229
Fragments arranged by size:
2229 1545 1296 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5257
PciI A'CATG_T
Cuts at: 4156 4156
Size: 5257
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5257
PmeI GTTT'AAAC
Cuts at: 2561 2561
Size: 5257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2123 2155 3326 3358 4839 4871 2123
Size: 32 1171 32 1481 32 2509
Fragments arranged by size:
2509 1481 1171 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5257
PsiI TTA'TAA
Cuts at: 2827 2827
Size: 5257
PspOMI G'GGCC_C
Cuts at: 2371 2413 2419 2371
Size: 42 6 5209
Fragments arranged by size:
5209 42 6
PstI C_TGCA'G
Cuts at: 830 2609 830
Size: 1779 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5257
PvuII CAG'CTG
Cuts at: 2119 2128 2119
Size: 9 5248
RsrII CG'GwC_CG
Cuts at: 2378 2378
Size: 5257
SalI G'TCGA_C
Cuts at: 1936 2599 1936
Size: 663 4594
SbfI CC_TGCA'GG
Cuts at: 2609 2609
Size: 5257
ScaI AGT'ACT
Cuts at: 3505 5217 3505
Size: 1712 3545
SfcI C'TryA_G
Cuts at: 826 1045 2605 3740 4421 4612 826
Size: 219 1560 1135 681 191 1471
Fragments arranged by size:
1560 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2252 2252
Size: 5257
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5257
SmaI CCC'GGG
Cuts at: 1872 2019 2418 2424 2584 1872
Size: 147 399 6 160 4545
Fragments arranged by size:
4545 399 160 147 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3361 4262 4524 820
Size: 197 926 1418 901 262 1553
Fragments arranged by size:
1553 1418 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3048 493
Size: 2555 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5257
SphI G_CATG'C
Cuts at: 1132 2615 1132
Size: 1483 3774
SrfI GCCC'GGGC
Cuts at: 2019 2418 2019
Size: 399 4858
SspI AAT'ATT
Cuts at: 5 52 3181 5
Size: 47 3129 2081
Fragments arranged by size:
3129 2081 47
StuI AGG'CCT
Cuts at: 1517 2147 2397 1517
Size: 630 250 4377
Fragments arranged by size:
4377 630 250
StyI C'CwwG_G
Cuts at: 513 1065 2190 2514 2696 513
Size: 552 1125 324 182 3074
Fragments arranged by size:
3074 1125 552 324 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3284 3301 3454 3639 4084 325
Size: 1255 1704 17 153 185 445 1498
Fragments arranged by size:
1704 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2166 3503 5215
Size: 274 80 33 51 1443 189 1337 1712
Cuts at: 5215 96
Size: 138
Fragments arranged by size:
1712 1443 1337 274 189 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5217 5249 5217
Size: 32 5225
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5257
XbaI T'CTAG_A
Cuts at: 2593 2593
Size: 5257
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5257
XmaI C'CCGG_G
Cuts at: 1870 2017 2416 2422 2582 1870
Size: 147 399 6 160 4545
Fragments arranged by size:
4545 399 160 147 6
XmnI GAAnn'nnTTC
Cuts at: 3386 3386
Size: 5257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3065 276
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacI
SacII SanDI SapI SexAI SfiI SwaI XcmI