################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AccI AccIII AclI AcsI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BbrPI BciVI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DraI DraIII Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MflI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1410 GACGTC GACGT^C Acc65I 671 GGTACC G^GTACC AlwNI 172 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 424 CCTGAGG CC^T[ACGT]AGG Aor51HI 1298 AGCGCT AGC^GCT Asp718I 671 GGTACC G^GTACC BbeI 1120 GGCGCC GGCGC^C BbsI 637 GAAGAC GAAGAC(2/6) BbvCI 1311 CCTCAGC CCTCAGC(-5/-2) BcgI 1556 GCATCCGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 637 GAAGAC GAAGAC(2/6) BsaAI 991 CACGTA [CT]AC^GT[GA] BseRI 1525 CTCCTC GAGGAG(10/8) BsiEI 963 CGGCCG CG[GA][CT]^CG BsmI 358 GAATGC GAATGC(1/-1) BsrDI 252 CATTGC GCAATG(2/0) BstAPI 1511 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 424 CCTGAGG CC^T[ACGT]AGG DrdI 799 GACCTGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 963 CGGCCG C^GGCCG EclXI 963 CGGCCG C^GGCCG Eco47III 1298 AGCGCT AGC^GCT Eco52I 963 CGGCCG C^GGCCG Eco81I 424 CCTGAGG CC^T[ACGT]AGG EcoNI 115 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG KasI 1120 GGCGCC G^GCGCC KpnI 671 GGTACC GGTAC^C NaeI 945 GCCGGC GCC^GGC NarI 1120 GGCGCC GG^CGCC NgoMIV 945 GCCGGC G^CCGGC PaeR7I 1189 CTCGAG C^TCGAG PstI 1518 CTGCAG CTGCA^G SacI 498 GAGCTC GAGCT^C SfiI 735 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1120 GGCGCC GGC^GCC SmaI 737 CCCGGG CCC^GGG TfiI 442 GATTC G^A[AT]TC Tsp509I 15 AATT ^AATT XbaI 393 TCTAGA T^CTAGA XhoI 1189 CTCGAG C^TCGAG XmaCI 737 CCCGGG C^CCGGG XmaI 737 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AfaI 672 GTAC GT^AC 1719 GTAC Alw44I 1105 GTGCAC G^TGCAC 1183 GTGCAC AlwI 23 GATCC GGATC(4/5) 821 GGATC ApaLI 1105 GTGCAC G^TGCAC 1183 GTGCAC BalI 1035 TGGCCA TGG^CCA 1604 TGGCCA BfaI 394 CTAG C^TAG 541 CTAG BglI 736 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1094 GCCAGGTGGGC BlpI 478 GCTGAGC GC^T[ACGT]AGC 1294 GCTGAGC BpmI 38 CTCCAG CTGGAG(16/14) 526 CTGGAG Bpu1102I 478 GCTGAGC GC^T[ACGT]AGC 1294 GCTGAGC BsaI 583 GAGACC GGTCTC(1/5) 775 GAGACC BsgI 1279 CTGCAC GTGCAG(16/14) 181 GTGCAG BspMI 332 ACCTGC ACCTGC(4/8) 1274 ACCTGC BsrFI 945 GCCGGC [GA]^CCGG[CT] 1466 ACCGGC CelII 478 GCTGAGC GC^T[ACGT]AGC 1294 GCTGAGC Cfr10I 945 GCCGGC [GA]^CCGG[CT] 1466 ACCGGC EarI 385 GAAGAG CTCTTC(1/4) 553 GAAGAG HaeII 1120 GGCGCC [GA]GCGC^[CT] 1298 AGCGCT Ksp632I 385 GAAGAG CTCTTC(1/4) 553 GAAGAG MaeI 394 CTAG C^TAG 541 CTAG MaeII 992 ACGT A^CGT 1411 ACGT MluNI 1035 TGGCCA TGG^CCA 1604 TGGCCA MscI 1035 TGGCCA TGG^CCA 1604 TGGCCA MslI 69 CATTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 720 CACGCGCATG PflMI 264 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 559 CCACCCGCTGG RsaI 672 GTAC GT^AC 1719 GTAC SapI 385 GAAGAGC GCTCTTC(1/4) 553 GAAGAGC SfcI 1056 CTACAG C^T[GA][CT]AG 1518 CTGCAG SmlI 501 CTCAAG C^T[CT][GA]AG 1189 CTCGAG StuI 1040 AGGCCT AGG^CCT 1643 AGGCCT TaqI 1190 TCGA T^CGA 1628 TCGA TthHB8I 1190 TCGA T^CGA 1628 TCGA Van91I 264 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 559 CCACCCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AspHI AspI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflFI PleI PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI Tth111I XcmI