(Circular) (Six-base) MAPSORT of: pFN21AE5684.seq Check: 7643 from: 1 to: 6430 pFN21AE5684 6430 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3419 4240 278 Size: 53 83 186 936 1883 821 2468 Fragments arranged by size: 2468 1883 936 821 186 83 53 AccI GT'mk_AC Cuts at: 1937 3773 1937 Size: 1836 4594 Acc65I G'GTAC_C Cuts at: 2676 3751 2676 Size: 1075 5355 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 2409 2577 4493 5877 6145 1632 Size: 527 250 168 1916 1384 268 1917 Fragments arranged by size: 1917 1916 1384 527 268 250 168 AfeI AGC'GCT Cuts at: 3305 3305 Size: 6430 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6430 AlwNI CAG_nnn'CTG Cuts at: 2182 4173 5745 2182 Size: 1991 1572 2867 Fragments arranged by size: 2867 1991 1572 ApaI G_GGCC'C Cuts at: 2067 2225 3316 2067 Size: 158 1091 5181 Fragments arranged by size: 5181 1091 158 ApaLI G'TGCA_C Cuts at: 3110 3188 4487 5643 3110 Size: 78 1299 1156 3897 Fragments arranged by size: 3897 1299 1156 78 ApoI r'AATT_y Cuts at: 1493 3739 3965 4117 6226 1493 Size: 2246 226 152 2109 1697 Fragments arranged by size: 2246 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2585 2742 3115 3194 3307 3755 1870 Size: 73 642 157 373 79 113 448 4545 Fragments arranged by size: 4545 642 448 373 157 113 79 73 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2539 2573 2620 2676 2953 Size: 325 217 627 752 34 47 56 277 Cuts at: 2953 3125 3751 5206 618 Size: 172 626 1455 1842 Fragments arranged by size: 1842 1455 752 627 626 325 277 217 172 56 47 34 BanII G_rGCy'C Cuts at: 1391 2067 2225 2242 2310 2507 2585 3122 3251 Size: 676 158 17 68 197 78 537 129 Cuts at: 3251 3316 3749 1391 Size: 65 433 4072 Fragments arranged by size: 4072 676 537 433 197 158 129 78 68 65 17 BbeI G_GCGC'C Cuts at: 3129 3129 Size: 6430 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2649 928 Size: 971 750 4709 Fragments arranged by size: 4709 971 750 BbvCI CC'TCA_GC Cuts at: 3317 3317 Size: 6430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3550 3584 4621 4655 1903 Size: 34 1613 34 1037 34 3678 Fragments arranged by size: 3678 1613 1037 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2747 3105 5041 Size: 107 122 71 631 605 1075 358 1936 Cuts at: 5041 136 Size: 1525 Fragments arranged by size: 1936 1525 1075 631 605 358 122 107 71 BglII A'GATC_T Cuts at: 6425 6425 Size: 6430 BlpI GC'TnA_GC Cuts at: 2484 3300 3847 2484 Size: 816 547 5067 Fragments arranged by size: 5067 816 547 Bme1580I G_kGCm'C Cuts at: 1790 2067 2225 2578 3114 3192 3316 4491 5647 Size: 277 158 353 536 78 124 1175 1156 Cuts at: 5647 1790 Size: 2573 Fragments arranged by size: 2573 1175 1156 536 353 277 158 124 78 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6430 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4161 5119 442 Size: 416 53 83 399 24 2744 958 1753 Fragments arranged by size: 2744 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2494 2494 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2028 2552 5090 1239 Size: 789 524 2538 2579 Fragments arranged by size: 2579 2538 789 524 Bpu10I CC'TnA_GC Cuts at: 3317 3317 Size: 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2491 4555 5420 5718 2491 Size: 2064 865 298 3203 Fragments arranged by size: 3203 2064 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2582 2774 5093 882 Size: 617 1083 192 2319 2219 Fragments arranged by size: 2319 2219 1083 617 192 BsaAI yAC'GTr Cuts at: 493 1204 2998 4221 493 Size: 711 1794 1223 2702 Fragments arranged by size: 2702 1794 1223 711 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3015 3126 3416 4237 Size: 53 83 186 936 1482 111 290 821 Cuts at: 4237 4619 5254 275 Size: 382 635 1451 Fragments arranged by size: 1482 1451 936 821 635 382 290 186 111 83 53 BsaWI w'CCGG_w Cuts at: 4863 5261 5535 5682 6093 4863 Size: 398 274 147 411 5200 Fragments arranged by size: 5200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3146 3176 1178 Size: 30 165 30 1743 30 4432 Fragments arranged by size: 4432 1743 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3521 1186 Size: 2335 4095 BseYI C'CCAG_C Cuts at: 2341 2691 2784 3018 3095 3243 3493 5633 6129 Size: 350 93 234 77 148 250 2140 496 Cuts at: 6129 2341 Size: 2642 Fragments arranged by size: 2642 2140 496 350 250 234 148 93 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2207 3269 1163 Size: 573 471 1062 4324 Fragments arranged by size: 4324 1062 573 471 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2971 3880 4641 5669 1540 Size: 275 186 970 909 761 1028 2301 Fragments arranged by size: 2301 1028 970 909 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2507 3114 3192 4491 4576 5647 2507 Size: 607 78 1299 85 1071 3290 Fragments arranged by size: 3290 1299 1071 607 85 78 BsmI GAATG_Cn' Cuts at: 2369 3941 4034 2369 Size: 1572 93 4765 Fragments arranged by size: 4765 1572 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2528 2818 3395 2528 Size: 290 577 5563 Fragments arranged by size: 5563 577 290 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2225 2242 2310 2507 2578 2585 Size: 399 277 158 17 68 197 71 7 Cuts at: 2585 3114 3122 3192 3251 3316 3749 4491 4576 Size: 529 8 70 59 65 433 742 85 Cuts at: 4576 5647 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 529 433 399 277 197 158 85 71 70 68 65 59 17 8 7 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 4319 6049 4319 Size: 1730 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 3288 3785 844 Size: 1502 942 497 3489 Fragments arranged by size: 3489 1502 942 497 BsrBI CCG'CTC Cuts at: 1394 1823 4317 1394 Size: 429 2494 3507 Fragments arranged by size: 3507 2494 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2256 4925 5099 65 Size: 1183 1008 2669 174 1396 Fragments arranged by size: 2669 1396 1183 1008 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2950 3471 5074 5261 1700 Size: 110 1140 521 1603 187 2869 Fragments arranged by size: 2869 1603 1140 521 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 4490 5502 4490 Size: 1012 5418 BstAPI GCAn_nnn'nTGC Cuts at: 794 3522 794 Size: 2728 3702 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6430 BstXI CCAn_nnnn'nTGG Cuts at: 2056 2680 2695 3038 3103 3361 2056 Size: 624 15 343 65 258 5125 Fragments arranged by size: 5125 624 343 258 65 15 BstYI r'GATC_y Cuts at: 1972 3760 4127 4512 4529 5981 6067 6079 6425 Size: 1788 367 385 17 1452 86 12 346 Cuts at: 6425 1972 Size: 1977 Fragments arranged by size: 1977 1788 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2430 2430 Size: 6430 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 2269 2302 2751 2965 3535 Size: 552 939 45 220 33 449 214 570 Cuts at: 3535 513 Size: 3408 Fragments arranged by size: 3408 939 570 552 449 220 214 45 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3010 3256 3529 487 Size: 683 56 1784 246 273 3388 Fragments arranged by size: 3388 1784 683 273 246 56 BtsI GCAGTG_nn' Cuts at: 2353 3439 3946 4740 4760 2353 Size: 1086 507 794 20 4023 Fragments arranged by size: 4023 1086 794 507 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2810 5437 809 Size: 507 1494 2627 1802 Fragments arranged by size: 2627 1802 1494 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2326 2968 3040 Size: 54 1425 50 275 18 496 642 72 Cuts at: 3040 3571 3609 3877 4766 6395 8 Size: 531 38 268 889 1629 43 Fragments arranged by size: 1629 1425 889 642 531 496 275 268 72 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 2968 3877 1537 Size: 275 1156 909 4090 Fragments arranged by size: 4090 1156 909 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2385 2553 4360 1353 Size: 1032 168 1807 3423 Fragments arranged by size: 3423 1807 1032 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5017 5391 5537 387 Size: 1064 138 3428 374 146 1280 Fragments arranged by size: 3428 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2505 2505 Size: 6430 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 2159 2409 2552 2577 4493 5090 Size: 393 396 131 250 143 25 1916 597 Cuts at: 5090 5877 6145 1239 Size: 787 268 1524 Fragments arranged by size: 1916 1524 787 597 396 393 268 250 143 131 25 EcoNI CCTnn'n_nnAGG Cuts at: 2124 2124 Size: 6430 EcoO109I rG'GnC_Cy Cuts at: 2039 2063 2092 2116 2132 2434 2475 2767 3148 Size: 24 29 24 16 302 41 292 381 Cuts at: 3148 3214 3293 3312 3313 2039 Size: 66 79 19 1 5156 Fragments arranged by size: 5156 381 302 292 79 66 41 29 24 24 19 16 1 EcoRI G'AATT_C Cuts at: 3739 4117 3739 Size: 378 6052 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 3129 3307 5577 1678 Size: 1451 178 2270 2531 Fragments arranged by size: 2531 2270 1451 178 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2438 2470 2527 2559 3146 Size: 32 135 32 866 32 57 32 587 Cuts at: 3146 3178 3392 3424 5071 5103 5145 5177 1373 Size: 32 214 32 1647 32 42 32 2626 Fragments arranged by size: 2626 1647 866 587 214 135 57 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3774 4020 669 Size: 988 281 1836 246 3079 Fragments arranged by size: 3079 1836 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KasI G'GCGC_C Cuts at: 3125 3125 Size: 6430 KpnI G_GTAC'C Cuts at: 2680 3755 2680 Size: 1075 5355 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2129 2682 3189 5544 5728 1305 Size: 824 553 507 2355 184 2007 Fragments arranged by size: 2355 2007 824 553 507 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3042 3611 10 Size: 54 1425 343 1210 569 2829 Fragments arranged by size: 2829 1425 1210 569 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 2729 4388 4747 4906 518 Size: 648 912 651 1659 359 159 2042 Fragments arranged by size: 2042 1659 912 651 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2570 2695 2843 3392 3493 3683 3846 4523 Size: 964 125 148 549 101 190 163 677 Cuts at: 4523 5671 5916 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 964 677 549 245 190 163 148 125 101 NaeI GCC'GGC Cuts at: 2952 2952 Size: 6430 NarI GG'CG_CC Cuts at: 3126 3126 Size: 6430 NcoI C'CATG_G Cuts at: 513 1065 2004 2049 2269 2751 513 Size: 552 939 45 220 482 4192 Fragments arranged by size: 4192 939 552 482 220 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NgoMIV G'CCGG_C Cuts at: 2950 2950 Size: 6430 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 3788 5333 1132 Size: 97 2559 1545 2229 Fragments arranged by size: 2559 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2067 1348 Size: 719 5711 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 2570 1108 Size: 1167 295 4968 Fragments arranged by size: 4968 1167 295 PfoI T'CCnGG_A Cuts at: 2649 2649 Size: 6430 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2092 2116 2434 3148 2092 Size: 24 318 714 5374 Fragments arranged by size: 5374 714 318 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2063 2221 3312 2063 Size: 158 1091 5181 Fragments arranged by size: 5181 1091 158 PstI C_TGCA'G Cuts at: 830 3527 3782 830 Size: 2697 255 3478 Fragments arranged by size: 3478 2697 255 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2695 3493 3683 2695 Size: 798 190 5442 Fragments arranged by size: 5442 798 190 SacI G_AGCT'C Cuts at: 2507 2507 Size: 6430 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SanDI GG'GwC_CC Cuts at: 2116 2116 Size: 6430 SapI GCTCTTCn'nnn_ Cuts at: 2385 2553 2385 Size: 168 6262 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SexAI A'CCwGG_T Cuts at: 3726 3726 Size: 6430 SfcI C'TryA_G Cuts at: 826 1045 3061 3523 3778 4913 5594 5785 826 Size: 219 2016 462 255 1135 681 191 1471 Fragments arranged by size: 2016 1471 1135 681 462 255 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2747 2747 Size: 6430 SfoI GGC'GCC Cuts at: 3127 3127 Size: 6430 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6430 SmaI CCC'GGG Cuts at: 1872 2744 3757 1872 Size: 872 1013 4545 Fragments arranged by size: 4545 1013 872 SmlI C'TyrA_G Cuts at: 820 1017 1943 2506 3194 4534 5435 5697 820 Size: 197 926 563 688 1340 901 262 1553 Fragments arranged by size: 1553 1340 926 901 688 563 262 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 3047 3650 1517 Size: 1530 603 4297 Fragments arranged by size: 4297 1530 603 StyI C'CwwG_G Cuts at: 513 1065 2004 2049 2269 2751 3869 513 Size: 552 939 45 220 482 1118 3074 Fragments arranged by size: 3074 1118 939 552 482 220 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2455 2705 4457 4474 4627 4812 5257 Size: 1255 875 250 1752 17 153 185 445 Cuts at: 5257 325 Size: 1498 Fragments arranged by size: 1752 1498 1255 875 445 250 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4676 6388 96 Size: 274 80 33 51 1443 2699 1712 138 Fragments arranged by size: 2699 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6390 6422 6390 Size: 32 6398 Tth111I GACn'n_nGTC Cuts at: 1316 2657 2759 3406 1316 Size: 1341 102 647 4340 Fragments arranged by size: 4340 1341 647 102 XbaI T'CTAG_A Cuts at: 2398 3766 2398 Size: 1368 5062 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2621 2728 2797 3140 2621 Size: 107 69 343 5911 Fragments arranged by size: 5911 343 107 69 XhoI C'TCGA_G Cuts at: 1943 3194 1943 Size: 1251 5179 XmaI C'CCGG_G Cuts at: 1870 2742 3755 1870 Size: 872 1013 4545 Fragments arranged by size: 4545 1013 872 XmnI GAAnn'nnTTC Cuts at: 4559 4559 Size: 6430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3417 4238 276 Size: 53 83 186 936 1883 821 2468 Fragments arranged by size: 2468 1883 936 821 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SrfI SwaI