(Linear) (Six-base) MAPSORT of: KIEE5684.seq Check: 8998 from: 1 to: 1725 KIEE5684.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AatII G_ACGT'C Cuts at: 0 1414 1725 Size: 1414 311 Acc65I G'GTAC_C Cuts at: 0 671 1725 Size: 671 1054 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 404 572 1725 Size: 154 250 168 1153 Fragments arranged by size: 1153 250 168 154 AfeI AGC'GCT Cuts at: 0 1300 1725 Size: 1300 425 AlwNI CAG_nnn'CTG Cuts at: 0 177 1725 Size: 177 1548 ApaI G_GGCC'C Cuts at: 0 62 220 1311 1725 Size: 62 158 1091 414 Fragments arranged by size: 1091 414 158 62 ApaLI G'TGCA_C Cuts at: 0 1105 1183 1725 Size: 1105 78 542 Fragments arranged by size: 1105 542 78 AvaI C'yCGr_G Cuts at: 0 580 737 1110 1189 1302 1725 Size: 580 157 373 79 113 423 Fragments arranged by size: 580 423 373 157 113 79 BanI G'GyrC_C Cuts at: 0 534 568 615 671 948 1120 1725 Size: 534 34 47 56 277 172 605 Fragments arranged by size: 605 534 277 172 56 47 34 BanII G_rGCy'C Cuts at: 0 62 220 237 305 502 580 1117 1246 Size: 62 158 17 68 197 78 537 129 Cuts at: 1246 1311 1725 Size: 65 414 Fragments arranged by size: 537 414 197 158 129 78 68 65 62 17 BbeI G_GCGC'C Cuts at: 0 1124 1725 Size: 1124 601 BbsI GAAGACnn'nnnn_ Cuts at: 0 644 1725 Size: 644 1081 BbvCI CC'TCA_GC Cuts at: 0 1312 1725 Size: 1312 413 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1545 1579 1725 Size: 1545 34 146 Fragments arranged by size: 1545 146 34 BglI GCCn_nnn'nGGC Cuts at: 0 742 1100 1725 Size: 742 358 625 Fragments arranged by size: 742 625 358 BlpI GC'TnA_GC Cuts at: 0 479 1295 1725 Size: 479 816 430 Fragments arranged by size: 816 479 430 Bme1580I G_kGCm'C Cuts at: 0 62 220 573 1109 1187 1311 1725 Size: 62 158 353 536 78 124 414 Fragments arranged by size: 536 414 353 158 124 78 62 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 489 1725 Size: 489 1236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 23 547 1725 Size: 23 524 1178 Fragments arranged by size: 1178 524 23 Bpu10I CC'TnA_GC Cuts at: 0 1312 1725 Size: 1312 413 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 486 1725 Size: 486 1239 BsaI GGTCTCn'nnnn_ Cuts at: 0 577 769 1725 Size: 577 192 956 Fragments arranged by size: 956 577 192 BsaAI yAC'GTr Cuts at: 0 993 1725 Size: 993 732 BsaHI Gr'CG_yC Cuts at: 0 1010 1121 1411 1725 Size: 1010 111 290 314 Fragments arranged by size: 1010 314 290 111 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1141 1171 1725 Size: 1141 30 554 Fragments arranged by size: 1141 554 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1516 1725 Size: 1516 209 BseYI C'CCAG_C Cuts at: 0 336 686 779 1013 1090 1238 1488 1725 Size: 336 350 93 234 77 148 250 237 Fragments arranged by size: 350 336 250 237 234 148 93 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 1264 1725 Size: 202 1062 461 Fragments arranged by size: 1062 461 202 BsiEI CG_ry'CG Cuts at: 0 966 1725 Size: 966 759 BsiHKAI G_wGCw'C Cuts at: 0 502 1109 1187 1725 Size: 502 607 78 538 Fragments arranged by size: 607 538 502 78 BsmI GAATG_Cn' Cuts at: 0 364 1725 Size: 364 1361 BsmBI CGTCTCn'nnnn_ Cuts at: 0 523 813 1390 1725 Size: 523 290 577 335 Fragments arranged by size: 577 523 335 290 Bsp1286I G_dGCh'C Cuts at: 0 62 220 237 305 502 573 580 1109 Size: 62 158 17 68 197 71 7 529 Cuts at: 1109 1117 1187 1246 1311 1725 Size: 8 70 59 65 414 Fragments arranged by size: 529 414 197 158 71 70 68 65 62 59 17 8 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 341 1283 1725 Size: 341 942 442 Fragments arranged by size: 942 442 341 BsrDI GCAATG_nn' Cuts at: 0 251 1725 Size: 251 1474 BsrFI r'CCGG_y Cuts at: 0 945 1466 1725 Size: 945 521 259 Fragments arranged by size: 945 521 259 BstAPI GCAn_nnn'nTGC Cuts at: 0 1517 1725 Size: 1517 208 BstXI CCAn_nnnn'nTGG Cuts at: 0 51 675 690 1033 1098 1356 1725 Size: 51 624 15 343 65 258 369 Fragments arranged by size: 624 369 343 258 65 51 15 Bsu36I CC'TnA_GG Cuts at: 0 425 1725 Size: 425 1300 BtgI C'CryG_G Cuts at: 0 44 264 297 746 960 1530 1725 Size: 44 220 33 449 214 570 195 Fragments arranged by size: 570 449 220 214 195 44 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1005 1251 1524 1725 Size: 1005 246 273 201 Fragments arranged by size: 1005 273 246 201 BtsI GCAGTG_nn' Cuts at: 0 348 1434 1725 Size: 348 1086 291 Fragments arranged by size: 1086 348 291 DrdI GACnn_nn'nnGTC Cuts at: 0 805 1725 Size: 805 920 EaeI y'GGCC_r Cuts at: 0 321 963 1035 1566 1604 1725 Size: 321 642 72 531 38 121 Fragments arranged by size: 642 531 321 121 72 38 EagI C'GGCC_G Cuts at: 0 963 1725 Size: 963 762 EarI CTCTTCn'nnn_ Cuts at: 0 380 548 1725 Size: 380 168 1177 Fragments arranged by size: 1177 380 168 EcoICRI GAG'CTC Cuts at: 0 500 1725 Size: 500 1225 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 23 154 404 547 572 1725 Size: 23 131 250 143 25 1153 Fragments arranged by size: 1153 250 143 131 25 23 EcoNI CCTnn'n_nnAGG Cuts at: 0 119 1725 Size: 119 1606 EcoO109I rG'GnC_Cy Cuts at: 0 34 58 87 111 127 429 470 762 Size: 34 24 29 24 16 302 41 292 Cuts at: 762 1143 1209 1288 1307 1308 1725 Size: 381 66 79 19 1 417 Fragments arranged by size: 417 381 302 292 79 66 41 34 29 24 24 19 16 1 HaeII r_GCGC'y Cuts at: 0 1124 1302 1725 Size: 1124 178 423 Fragments arranged by size: 1124 423 178 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 433 465 522 554 1141 1173 1387 1419 Size: 433 32 57 32 587 32 214 32 Cuts at: 1419 1725 Size: 306 Fragments arranged by size: 587 433 306 214 57 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1120 1725 Size: 1120 605 KpnI G_GTAC'C Cuts at: 0 675 1725 Size: 675 1050 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 124 677 1184 1725 Size: 124 553 507 541 Fragments arranged by size: 553 541 507 124 MscI TGG'CCA Cuts at: 0 1037 1606 1725 Size: 1037 569 119 Fragments arranged by size: 1037 569 119 MslI CAynn'nnrTG Cuts at: 0 73 724 1725 Size: 73 651 1001 Fragments arranged by size: 1001 651 73 MspA1I CmG'CkG Cuts at: 0 565 690 838 1387 1488 1678 1725 Size: 565 125 148 549 101 190 47 Fragments arranged by size: 565 549 190 148 125 101 47 NaeI GCC'GGC Cuts at: 0 947 1725 Size: 947 778 NarI GG'CG_CC Cuts at: 0 1121 1725 Size: 1121 604 NcoI C'CATG_G Cuts at: 0 44 264 746 1725 Size: 44 220 482 979 Fragments arranged by size: 979 482 220 44 NgoMIV G'CCGG_C Cuts at: 0 945 1725 Size: 945 780 PasI CC'CwG_GG Cuts at: 0 62 1725 Size: 62 1663 PflMI CCAn_nnn'nTGG Cuts at: 0 270 565 1725 Size: 270 295 1160 Fragments arranged by size: 1160 295 270 PfoI T'CCnGG_A Cuts at: 0 644 1725 Size: 644 1081 PpuMI rG'GwC_Cy Cuts at: 0 87 111 429 1143 1725 Size: 87 24 318 714 582 Fragments arranged by size: 714 582 318 87 24 PspOMI G'GGCC_C Cuts at: 0 58 216 1307 1725 Size: 58 158 1091 418 Fragments arranged by size: 1091 418 158 58 PstI C_TGCA'G Cuts at: 0 1522 1725 Size: 1522 203 PvuII CAG'CTG Cuts at: 0 690 1488 1678 1725 Size: 690 798 190 47 Fragments arranged by size: 798 690 190 47 SacI G_AGCT'C Cuts at: 0 502 1725 Size: 502 1223 SanDI GG'GwC_CC Cuts at: 0 111 1725 Size: 111 1614 SapI GCTCTTCn'nnn_ Cuts at: 0 380 548 1725 Size: 380 168 1177 Fragments arranged by size: 1177 380 168 SfcI C'TryA_G Cuts at: 0 1056 1518 1725 Size: 1056 462 207 Fragments arranged by size: 1056 462 207 SfiI GGCCn_nnn'nGGCC Cuts at: 0 742 1725 Size: 742 983 SfoI GGC'GCC Cuts at: 0 1122 1725 Size: 1122 603 SmaI CCC'GGG Cuts at: 0 739 1725 Size: 739 986 SmlI C'TyrA_G Cuts at: 0 501 1189 1725 Size: 501 688 536 Fragments arranged by size: 688 536 501 StuI AGG'CCT Cuts at: 0 1042 1645 1725 Size: 1042 603 80 Fragments arranged by size: 1042 603 80 StyI C'CwwG_G Cuts at: 0 44 264 746 1725 Size: 44 220 482 979 Fragments arranged by size: 979 482 220 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 450 700 1725 Size: 450 250 1025 Fragments arranged by size: 1025 450 250 Tth111I GACn'n_nGTC Cuts at: 0 652 754 1401 1725 Size: 652 102 647 324 Fragments arranged by size: 652 647 324 102 XbaI T'CTAG_A Cuts at: 0 393 1725 Size: 393 1332 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 616 723 792 1135 1725 Size: 616 107 69 343 590 Fragments arranged by size: 616 590 343 107 69 XhoI C'TCGA_G Cuts at: 0 1189 1725 Size: 1189 536 XmaI C'CCGG_G Cuts at: 0 737 1725 Size: 737 988 ZraI GAC'GTC Cuts at: 0 1412 1725 Size: 1412 313 Enzymes that do cut: AatII Acc65I AcuI AfeI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SapI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AarI AccI AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BsaBI BsaWI BsiWI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DraIII EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XmnI