################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AlwNI AocI ApoI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MboI MfeI MflI MluI MroI MseI MslI MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AfaI 671 GTAC GT^AC AluI 518 AGCT AG^CT Alw44I 73 GTGCAC G^TGCAC ApaLI 73 GTGCAC G^TGCAC AscI 135 GGCGCGCC GG^CGCGCC Asp700I 552 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbvCI 311 CCTCAGC CCTCAGC(-5/-2) BsaWI 533 ACCGGA [AT]^CCGG[AT] BsiEI 8 CGGCCG CG[GA][CT]^CG BsrBI 507 CCGCTC CCGCTC(-3/-3) BsrFI 115 GCCGGC [GA]^CCGG[CT] BsrI 479 ACTGG ACTGG(1/-1) BstXI 242 CCAGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 115 GCCGGC [GA]^CCGG[CT] DdeI 312 CTCAG C^T[ACGT]AG EagI 8 CGGCCG C^GGCCG EarI 57 CTCTTC CTCTTC(1/4) EclXI 8 CGGCCG C^GGCCG Eco52I 8 CGGCCG C^GGCCG HinfI 685 GACTC G^A[ACGT]TC Ksp632I 57 CTCTTC CTCTTC(1/4) MaeII 41 ACGT A^CGT MboII 58 TCTTC GAAGA(8/7) NaeI 115 GCCGGC GCC^GGC NgoMIV 115 GCCGGC G^CCGGC NlaIII 48 CATG CATG^ PflMI 228 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 685 GACTC GAGTC(4/5) PpuMI 160 GGGACCC [GA]G^G[AT]CC[CT] PstI 696 CTGCAG CTGCA^G RsaI 671 GTAC GT^AC SfaNI 245 GCATC GCATC(5/9) SfcI 696 CTGCAG C^T[GA][CT]AG StuI 750 AGGCCT AGG^CCT TspRI 594 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 228 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 552 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### Aor51HI 554 AGCGCT AGC^GCT 581 AGCGCT ApaI 105 GGGCCC GGGCC^C 204 GGGCCC BalI 50 TGGCCA TGG^CCA 605 TGGCCA BssHII 129 GCGCGC G^CGCGC 136 GCGCGC BstF5I 246 CATCC GGATG(2/0) 123 GGATG Eco47III 554 AGCGCT AGC^GCT 581 AGCGCT FokI 246 CATCC GGATG(9/13) 123 GGATG HgaI 447 GACGC GACGC(5/10) 537 GACGC HphI 154 TCACC GGTGA(8/7) 587 TCACC KspI 78 CCGCGG CCGC^GG 218 CCGCGG MaeIII 476 GTGAC ^GT[ACGT]AC 596 GTGAC MluNI 50 TGGCCA TGG^CCA 605 TGGCCA MscI 50 TGGCCA TGG^CCA 605 TGGCCA SacII 78 CCGCGG CCGC^GG 218 CCGCGG Tsp45I 476 GTGAC ^GT[GC]AC 596 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AcyI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV Sau96I ScrFI SfoI SmaI TseI XmaCI XmaI