(Circular) (Six-base) MAPSORT of: pFN21AB9693.seq Check: 2530 from: 1 to: 5461 pFN21AB9693 5461 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2244 2454 2244 Size: 210 5251 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2804 1937 Size: 867 4594 Acc65I G'GTAC_C Cuts at: 2782 2782 Size: 5461 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3524 4908 5176 1632 Size: 1892 1384 268 1917 Fragments arranged by size: 1917 1892 1384 268 AfeI AGC'GCT Cuts at: 2561 2588 2561 Size: 27 5434 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5264 AflIII A'CryG_T Cuts at: 1203 4360 5337 1203 Size: 3157 977 1327 Fragments arranged by size: 3157 1327 977 AgeI A'CCGG_T Cuts at: 4292 4292 Size: 5461 AhdI GACnn_n'nnGTC Cuts at: 4190 4190 Size: 5461 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5461 AlwNI CAG_nnn'CTG Cuts at: 3204 4776 3204 Size: 1572 3889 ApaI G_GGCC'C Cuts at: 2114 2213 2114 Size: 99 5362 ApaLI G'TGCA_C Cuts at: 2078 3518 4674 2078 Size: 1440 1156 2865 Fragments arranged by size: 2865 1440 1156 ApoI r'AATT_y Cuts at: 1493 2770 2996 3148 5257 1493 Size: 1277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 226 152 AscI GG'CGCG_CC Cuts at: 2141 2141 Size: 5461 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2099 2162 2170 2206 2287 2338 2786 Size: 73 156 63 8 36 81 51 448 Cuts at: 2786 1870 Size: 4545 Fragments arranged by size: 4545 448 156 81 73 63 51 36 8 BamHI G'GATC_C Cuts at: 2791 3158 2791 Size: 367 5094 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2216 2227 2294 2354 Size: 325 217 627 282 147 11 67 60 Cuts at: 2354 2658 2666 2782 4237 618 Size: 304 8 116 1455 1842 Fragments arranged by size: 1842 1455 627 325 304 282 217 147 116 67 60 11 8 BanII G_rGCy'C Cuts at: 1391 2114 2213 2720 2780 1391 Size: 723 99 507 60 4072 Fragments arranged by size: 4072 723 507 99 60 BbeI G_GCGC'C Cuts at: 2073 2220 2231 2298 2358 2662 2073 Size: 147 11 67 60 304 4872 Fragments arranged by size: 4872 304 147 67 60 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4490 BbvCI CC'TCA_GC Cuts at: 2317 2317 Size: 5461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 1903 Size: 34 1715 34 3678 Fragments arranged by size: 3678 1715 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3353 4563 3353 Size: 1210 4251 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2103 2225 Size: 107 122 71 631 605 337 94 122 Cuts at: 2225 2634 4072 136 Size: 409 1438 1525 Fragments arranged by size: 1525 1438 631 605 409 337 122 122 107 94 71 BglII A'GATC_T Cuts at: 5456 5456 Size: 5461 BlpI GC'TnA_GC Cuts at: 2878 2878 Size: 5461 Bme1580I G_kGCm'C Cuts at: 1790 2082 2114 2213 2671 3522 4678 1790 Size: 292 32 99 458 851 1156 2573 Fragments arranged by size: 2573 1156 851 458 292 99 32 BmgBI CAC'GTC Cuts at: 1318 2047 1318 Size: 729 4732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3192 4150 442 Size: 416 53 83 399 24 1775 958 1753 Fragments arranged by size: 1775 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4121 1239 Size: 2882 2579 Bpu10I CC'TnA_GC Cuts at: 2317 2317 Size: 5461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3586 4451 4749 3586 Size: 865 298 4298 Fragments arranged by size: 4298 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4124 882 Size: 617 2625 2219 Fragments arranged by size: 2625 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3252 493 Size: 711 2048 2702 Fragments arranged by size: 2702 2048 711 BsaBI GATnn'nnATC Cuts at: 5455 5455 Size: 5461 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2217 2228 2295 Size: 53 83 186 936 537 147 11 67 Cuts at: 2295 2355 2659 3268 3650 4285 275 Size: 60 304 609 382 635 1451 Fragments arranged by size: 1451 936 635 609 537 382 304 186 147 83 67 60 53 11 BsaWI w'CCGG_w Cuts at: 2538 3894 4292 4566 4713 5124 2538 Size: 1356 398 274 147 411 2875 Fragments arranged by size: 2875 1356 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5236 Fragments arranged by size: 5236 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2299 2302 2305 1186 Size: 1113 3 3 4342 Fragments arranged by size: 4342 1113 3 3 BseYI C'CCAG_C Cuts at: 2684 2726 2738 2743 4664 5160 2684 Size: 42 12 5 1921 496 2985 Fragments arranged by size: 2985 1921 496 42 12 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4888 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 2911 3672 4700 1540 Size: 275 186 15 895 761 1028 2301 Fragments arranged by size: 2301 1028 895 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2052 2082 2582 3522 3607 4678 2052 Size: 30 500 940 85 1071 2835 Fragments arranged by size: 2835 1071 940 500 85 30 BsmI GAATG_Cn' Cuts at: 2972 3065 2972 Size: 93 5368 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2082 2114 2213 2582 2671 2720 Size: 399 262 30 32 99 369 89 49 Cuts at: 2720 2780 3522 3607 4678 1391 Size: 60 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 262 99 89 85 60 49 32 30 BspEI T'CCGG_A Cuts at: 5124 5124 Size: 5461 BspHI T'CATG_A Cuts at: 3350 5080 3350 Size: 1730 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2271 2454 2658 2816 844 Size: 1400 27 183 204 158 3489 Fragments arranged by size: 3489 1400 204 183 158 27 BsrBI CCG'CTC Cuts at: 1394 1823 2514 3348 1394 Size: 429 691 834 3507 Fragments arranged by size: 3507 834 691 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3956 4130 65 Size: 1183 2708 174 1396 Fragments arranged by size: 2708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 4105 4292 1700 Size: 110 310 1985 187 2869 Fragments arranged by size: 2869 1985 310 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5461 BssHII G'CGCG_C Cuts at: 1925 2134 2141 1925 Size: 209 7 5245 Fragments arranged by size: 5245 209 7 BssSI C'ACGA_G Cuts at: 3521 4533 3521 Size: 1012 4449 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5461 BstBI TT'CG_AA Cuts at: 4265 4265 Size: 5461 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5461 BstXI CCAn_nnnn'nTGG Cuts at: 2254 2254 Size: 5461 BstYI r'GATC_y Cuts at: 1972 2791 3158 3543 3560 5012 5098 5110 5456 Size: 819 367 385 17 1452 86 12 346 Cuts at: 5456 1972 Size: 1977 Fragments arranged by size: 1977 1452 819 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2016 2073 2083 2223 513 Size: 552 951 57 10 140 3751 Fragments arranged by size: 3751 951 552 140 57 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4722 Fragments arranged by size: 4722 683 56 BtsI GCAGTG_nn' Cuts at: 2605 2977 3771 3791 2605 Size: 372 794 20 4275 Fragments arranged by size: 4275 794 372 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5426 DraI TTT'AAA Cuts at: 2765 2765 Size: 5461 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2055 2241 Size: 54 1425 50 275 18 183 42 186 Cuts at: 2241 2256 2610 2709 2908 3797 5426 8 Size: 15 354 99 199 889 1629 43 Fragments arranged by size: 1629 1425 889 354 275 199 186 183 99 54 50 43 42 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2013 2908 1537 Size: 275 201 895 4090 Fragments arranged by size: 4090 895 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2068 3391 1353 Size: 715 1323 3423 Fragments arranged by size: 3423 1323 715 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2286 2356 4048 4422 4568 387 Size: 1064 138 697 70 1692 374 146 1280 Fragments arranged by size: 1692 1280 1064 697 374 146 138 70 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3524 4121 4908 5176 1239 Size: 393 1892 597 787 268 1524 Fragments arranged by size: 1892 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2111 2166 2210 2111 Size: 55 44 5362 Fragments arranged by size: 5362 55 44 EcoRI G'AATT_C Cuts at: 2770 3148 2770 Size: 378 5083 FspI TGC'GCA Cuts at: 3967 3967 Size: 5461 HaeII r_GCGC'y Cuts at: 1678 2073 2220 2231 2298 2358 2563 2590 2662 Size: 395 147 11 67 60 205 27 72 Cuts at: 2662 4608 1678 Size: 1946 2531 Fragments arranged by size: 2531 1946 395 205 147 72 67 60 27 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4102 4134 4176 4208 1373 Size: 32 135 32 2530 32 42 32 2626 Fragments arranged by size: 2626 2530 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3051 669 Size: 988 281 867 246 3079 Fragments arranged by size: 3079 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KasI G'GCGC_C Cuts at: 2069 2216 2227 2294 2354 2658 2069 Size: 147 11 67 60 304 4872 Fragments arranged by size: 4872 304 147 67 60 11 KpnI G_GTAC'C Cuts at: 2786 2786 Size: 5461 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2541 4575 4759 1305 Size: 1236 2034 184 2007 Fragments arranged by size: 2034 2007 1236 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 2612 10 Size: 54 1425 343 225 555 2859 Fragments arranged by size: 2859 1425 555 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 3419 3778 3937 518 Size: 648 2253 359 159 2042 Fragments arranged by size: 2253 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2225 2388 2877 3554 4702 4947 1606 Size: 479 140 163 489 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 489 479 245 163 140 NaeI GCC'GGC Cuts at: 2122 2122 Size: 5461 NarI GG'CG_CC Cuts at: 2070 2217 2228 2295 2355 2659 2070 Size: 147 11 67 60 304 4872 Fragments arranged by size: 4872 304 147 67 60 11 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4909 NdeI CA'TA_TG Cuts at: 387 387 Size: 5461 NgoMIV G'CCGG_C Cuts at: 2120 2120 Size: 5461 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2908 2908 Size: 5461 NspI r_CATG'y Cuts at: 1132 1229 2819 4364 1132 Size: 97 1590 1545 2229 Fragments arranged by size: 2229 1590 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5461 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 1108 Size: 1131 4330 PfoI T'CCnGG_A Cuts at: 2397 2397 Size: 5461 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2166 2166 Size: 5461 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PspOMI G'GGCC_C Cuts at: 2110 2209 2110 Size: 99 5362 PstI C_TGCA'G Cuts at: 830 2705 2813 830 Size: 1875 108 3478 Fragments arranged by size: 3478 1875 108 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 SacII CC_GC'GG Cuts at: 2086 2226 2086 Size: 140 5321 SalI G'TCGA_C Cuts at: 1936 2803 1936 Size: 867 4594 SanDI GG'GwC_CC Cuts at: 2166 2166 Size: 5461 SbfI CC_TGCA'GG Cuts at: 2813 2813 Size: 5461 ScaI AGT'ACT Cuts at: 3709 5421 3709 Size: 1712 3749 SfcI C'TryA_G Cuts at: 826 1045 2701 2809 3944 4625 4816 826 Size: 219 1656 108 1135 681 191 1471 Fragments arranged by size: 1656 1471 1135 681 219 191 108 SfoI GGC'GCC Cuts at: 2071 2218 2229 2296 2356 2660 2071 Size: 147 11 67 60 304 4872 Fragments arranged by size: 4872 304 147 67 60 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5461 SmaI CCC'GGG Cuts at: 1872 2164 2172 2208 2289 2788 1872 Size: 292 8 36 81 499 4545 Fragments arranged by size: 4545 499 292 81 36 8 SmlI C'TyrA_G Cuts at: 820 1017 1943 3565 4466 4728 820 Size: 197 926 1622 901 262 1553 Fragments arranged by size: 1622 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3252 493 Size: 2759 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2819 1132 Size: 1687 3774 SrfI GCCC'GGGC Cuts at: 2208 2208 Size: 5461 SspI AAT'ATT Cuts at: 5 52 3385 5 Size: 47 3333 2081 Fragments arranged by size: 3333 2081 47 StuI AGG'CCT Cuts at: 1517 2757 1517 Size: 1240 4221 StyI C'CwwG_G Cuts at: 513 1065 2900 513 Size: 552 1835 3074 Fragments arranged by size: 3074 1835 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3488 3505 3658 3843 4288 325 Size: 1255 1908 17 153 185 445 1498 Fragments arranged by size: 1908 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3707 5419 96 Size: 274 80 33 51 1443 1730 1712 138 Fragments arranged by size: 1730 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5421 5453 5421 Size: 32 5429 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5461 XbaI T'CTAG_A Cuts at: 2797 2797 Size: 5461 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5461 XmaI C'CCGG_G Cuts at: 1870 2162 2170 2206 2287 2786 1870 Size: 292 8 36 81 499 4545 Fragments arranged by size: 4545 499 292 81 36 8 XmnI GAAnn'nnTTC Cuts at: 2561 3590 2561 Size: 1029 4432 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SwaI XcmI