Restriction map for
pFN21AE1763
(Circular) (Six-base) MAPSORT of: pFN21AE1763.seq Check: 1463 from: 1 to: 5512
pFN21AE1763 5512 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2112 2112
Size: 5512
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3322 278
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2855 1937
Size: 918 4594
Acc65I G'GTAC_C
Cuts at: 2833 2833
Size: 5512
AclI AA'CG_TT
Cuts at: 1563 3639 4012 1563
Size: 2076 373 3063
Fragments arranged by size:
3063 2076 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 2607 3575 4959 5227 1632
Size: 872 103 968 1384 268 1917
Fragments arranged by size:
1917 1384 968 872 268 103
AfeI AGC'GCT
Cuts at: 2135 2135
Size: 5512
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5315
AflIII A'CryG_T
Cuts at: 1203 2409 4411 5388 1203
Size: 1206 2002 977 1327
Fragments arranged by size:
2002 1327 1206 977
AgeI A'CCGG_T
Cuts at: 4343 4343
Size: 5512
AhdI GACnn_n'nnGTC
Cuts at: 4241 4241
Size: 5512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5512
AlwNI CAG_nnn'CTG
Cuts at: 2746 3255 4827 2746
Size: 509 1572 3431
Fragments arranged by size:
3431 1572 509
ApaI G_GGCC'C
Cuts at: 2314 2314
Size: 5512
ApaLI G'TGCA_C
Cuts at: 3569 4725 3569
Size: 1156 4356
ApoI r'AATT_y
Cuts at: 1493 2821 3047 3199 5308 1493
Size: 1328 226 152 2109 1697
Fragments arranged by size:
2109 1697 1328 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4066 160
Size: 872 3034 1606
Fragments arranged by size:
3034 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
AvaI C'yCGr_G
Cuts at: 1870 1943 2837 1870
Size: 73 894 4545
Fragments arranged by size:
4545 894 73
BamHI G'GATC_C
Cuts at: 2397 2842 3209 2397
Size: 445 367 4700
Fragments arranged by size:
4700 445 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2245 2365 2833 4288
Size: 325 217 627 284 174 120 468 1455
Cuts at: 4288 618
Size: 1842
Fragments arranged by size:
1842 1455 627 468 325 284 217 174
120
BanII G_rGCy'C
Cuts at: 1391 2314 2831 1391
Size: 923 517 4072
Fragments arranged by size:
4072 923 517
BbeI G_GCGC'C
Cuts at: 2075 2249 2075
Size: 174 5338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2281 928
Size: 971 382 4159
Fragments arranged by size:
4159 971 382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2203 2237 3703 3737 1903
Size: 34 266 34 1466 34 3678
Fragments arranged by size:
3678 1466 266 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3404 4614 3404
Size: 1210 4302
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4123 136
Size: 107 122 71 631 605 2451 1525
Fragments arranged by size:
2451 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5507 5507
Size: 5512
BlpI GC'TnA_GC
Cuts at: 2929 2929
Size: 5512
Bme1580I G_kGCm'C
Cuts at: 1790 2314 3573 4729 1790
Size: 524 1259 1156 2573
Fragments arranged by size:
2573 1259 1156 524
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2317 3243 4201
Size: 416 53 83 399 24 900 926 958
Cuts at: 4201 442
Size: 1753
Fragments arranged by size:
1753 958 926 900 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2589 2664 4172 1239
Size: 980 370 75 1508 2579
Fragments arranged by size:
2579 1508 980 370 75
Bpu10I CC'TnA_GC
Cuts at: 2763 2763
Size: 5512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3637 4502 4800 3637
Size: 865 298 4349
Fragments arranged by size:
4349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 2423 2607 4175 882
Size: 617 636 288 184 1568 2219
Fragments arranged by size:
2219 1568 636 617 288 184
BsaAI yAC'GTr
Cuts at: 493 1204 2412 3303 493
Size: 711 1208 891 2702
Fragments arranged by size:
2702 1208 891 711
BsaBI GATnn'nnATC
Cuts at: 5506 5506
Size: 5512
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2072 2246 2286 2496
Size: 53 83 186 936 539 174 40 210
Cuts at: 2496 3319 3701 4336 275
Size: 823 382 635 1451
Fragments arranged by size:
1451 936 823 635 539 382 210 186
174 83 53 40
BsaWI w'CCGG_w
Cuts at: 3945 4343 4617 4764 5175 3945
Size: 398 274 147 411 4282
Fragments arranged by size:
4282 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2504 2534 1178
Size: 30 165 30 1101 30 4156
Fragments arranged by size:
4156 1101 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2167 2441 1186
Size: 981 274 4257
Fragments arranged by size:
4257 981 274
BseYI C'CCAG_C
Cuts at: 2015 2136 2777 4715 5211 2015
Size: 121 641 1938 496 2316
Fragments arranged by size:
2316 1938 641 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 1163
Size: 573 522 4417
Fragments arranged by size:
4417 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2962 3723 4751 1540
Size: 275 186 961 761 1028 2301
Fragments arranged by size:
2301 1028 961 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3573 3658 4729 3573
Size: 85 1071 4356
Fragments arranged by size:
4356 1071 85
BsmI GAATG_Cn'
Cuts at: 3023 3116 3023
Size: 93 5419
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2314 2831 3573 3658 4729 1391
Size: 399 524 517 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 524 517 399 85
BspEI T'CCGG_A
Cuts at: 5175 5175
Size: 5512
BspHI T'CATG_A
Cuts at: 3401 5131 3401
Size: 1730 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2867 844
Size: 1268 755 3489
Fragments arranged by size:
3489 1268 755
BsrBI CCG'CTC
Cuts at: 1394 1823 3399 1394
Size: 429 1576 3507
Fragments arranged by size:
3507 1576 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2284 4007 4181 65
Size: 1183 1036 1723 174 1396
Fragments arranged by size:
1723 1396 1183 1036 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2122 4156 4343 1700
Size: 110 312 2034 187 2869
Fragments arranged by size:
2869 2034 312 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5512
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5512
BssSI C'ACGA_G
Cuts at: 3572 4584 3572
Size: 1012 4500
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2746 794
Size: 1952 3560
BstBI TT'CG_AA
Cuts at: 4316 4316
Size: 5512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5512
BstXI CCAn_nnnn'nTGG
Cuts at: 2785 2785
Size: 5512
BstYI r'GATC_y
Cuts at: 1972 2397 2842 3209 3594 3611 5063 5149 5161
Size: 425 445 367 385 17 1452 86 12
Cuts at: 5161 5507 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 445 425 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2076 2315 2596 513
Size: 552 1011 239 281 3429
Fragments arranged by size:
3429 1011 552 281 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2206 487
Size: 683 56 980 3793
Fragments arranged by size:
3793 980 683 56
BtsI GCAGTG_nn'
Cuts at: 2747 2763 3028 3822 3842 2747
Size: 16 265 794 20 4417
Fragments arranged by size:
4417 794 265 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5512
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5477
DraI TTT'AAA
Cuts at: 2816 2816
Size: 5512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4519 809
Size: 507 3203 1802
Fragments arranged by size:
3203 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2217 2959
Size: 54 1425 50 275 18 333 54 742
Cuts at: 2959 3848 5477 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 742 333 275 54 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2959 1537
Size: 275 1147 4090
Fragments arranged by size:
4090 1147 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3442 1353
Size: 2089 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4099 4473 4619 387
Size: 1064 138 2510 374 146 1280
Fragments arranged by size:
2510 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2504 2589 2607 2664 3575 4172
Size: 393 587 285 85 18 57 911 597
Cuts at: 4172 4959 5227 1239
Size: 787 268 1524
Fragments arranged by size:
1524 911 787 597 587 393 285 268
85 57 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2354
Size: 5512
EcoO109I rG'GnC_Cy
Cuts at: 2194 2311 2669 2194
Size: 117 358 5037
Fragments arranged by size:
5037 358 117
EcoRI G'AATT_C
Cuts at: 2821 3199 2821
Size: 378 5134
FspI TGC'GCA
Cuts at: 2108 4018 2108
Size: 1910 3602
HaeII r_GCGC'y
Cuts at: 1678 2013 2075 2137 2249 4659 1678
Size: 335 62 62 112 2410 2531
Fragments arranged by size:
2531 2410 335 112 62 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4153 4185 4227 4259 1373
Size: 32 135 32 2581 32 42 32 2626
Fragments arranged by size:
2626 2581 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2856 3102 669
Size: 988 281 918 246 3079
Fragments arranged by size:
3079 988 918 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5512
HpaI GTT'AAC
Cuts at: 3102 3102
Size: 5512
KasI G'GCGC_C
Cuts at: 2071 2245 2071
Size: 174 5338
KpnI G_GTAC'C
Cuts at: 2837 2837
Size: 5512
MfeI C'AATT_G
Cuts at: 3111 3111
Size: 5512
MluI A'CGCG_T
Cuts at: 5388 5388
Size: 5512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4626 4810 1305
Size: 3321 184 2007
Fragments arranged by size:
3321 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2219 10
Size: 54 1425 343 387 3303
Fragments arranged by size:
3303 1425 387 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2391 2564 3470 3829 3988 518
Size: 648 1225 173 906 359 159 2042
Fragments arranged by size:
2042 1225 906 648 359 173 159
MspA1I CmG'CkG
Cuts at: 1606 2078 2093 2296 2928 3605 4753 4998 1606
Size: 472 15 203 632 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 632 472 245 203 15
NarI GG'CG_CC
Cuts at: 2072 2246 2072
Size: 174 5338
NcoI C'CATG_G
Cuts at: 513 1065 2596 513
Size: 552 1531 3429
Fragments arranged by size:
3429 1531 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5512
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5512
NotI GC'GGCC_GC
Cuts at: 2959 2959
Size: 5512
NspI r_CATG'y
Cuts at: 1132 1229 2870 4415 1132
Size: 97 1641 1545 2229
Fragments arranged by size:
2229 1641 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2685 1348
Size: 1337 4175
PciI A'CATG_T
Cuts at: 4411 4411
Size: 5512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5512
PfoI T'CCnGG_A
Cuts at: 2196 2301 2605 2196
Size: 105 304 5103
Fragments arranged by size:
5103 304 105
PmeI GTTT'AAAC
Cuts at: 2816 2816
Size: 5512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 5094 5126 3581
Size: 32 1481 32 3967
Fragments arranged by size:
3967 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2669 2194
Size: 475 5037
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5512
PsiI TTA'TAA
Cuts at: 3082 3082
Size: 5512
PspOMI G'GGCC_C
Cuts at: 2310 2310
Size: 5512
PstI C_TGCA'G
Cuts at: 830 2054 2864 830
Size: 1224 810 3478
Fragments arranged by size:
3478 1224 810
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5512
SacII CC_GC'GG
Cuts at: 2079 2079
Size: 5512
SalI G'TCGA_C
Cuts at: 1936 2854 1936
Size: 918 4594
SanDI GG'GwC_CC
Cuts at: 2669 2669
Size: 5512
SbfI CC_TGCA'GG
Cuts at: 2864 2864
Size: 5512
ScaI AGT'ACT
Cuts at: 2549 3760 5472 2549
Size: 1211 1712 2589
Fragments arranged by size:
2589 1712 1211
SfcI C'TryA_G
Cuts at: 826 1045 2050 2860 3995 4676 4867 826
Size: 219 1005 810 1135 681 191 1471
Fragments arranged by size:
1471 1135 1005 810 681 219 191
SfoI GGC'GCC
Cuts at: 2073 2247 2073
Size: 174 5338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5512
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5512
SmaI CCC'GGG
Cuts at: 1872 2839 1872
Size: 967 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3616 4517 4779 820
Size: 197 926 1673 901 262 1553
Fragments arranged by size:
1673 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3303 493
Size: 2810 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5512
SphI G_CATG'C
Cuts at: 1132 2870 1132
Size: 1738 3774
SspI AAT'ATT
Cuts at: 5 52 3436 5
Size: 47 3384 2081
Fragments arranged by size:
3384 2081 47
StuI AGG'CCT
Cuts at: 1517 2349 1517
Size: 832 4680
StyI C'CwwG_G
Cuts at: 513 1065 2596 2617 2951 513
Size: 552 1531 21 334 3074
Fragments arranged by size:
3074 1531 552 334 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2383 2677 3539 3556 3709 3894 4339
Size: 1255 803 294 862 17 153 185 445
Cuts at: 4339 325
Size: 1498
Fragments arranged by size:
1498 1255 862 803 445 294 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 3758 5470
Size: 274 80 33 51 1443 570 1211 1712
Cuts at: 5470 96
Size: 138
Fragments arranged by size:
1712 1443 1211 570 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5472 5504 5472
Size: 32 5480
Tth111I GACn'n_nGTC
Cuts at: 1316 2285 1316
Size: 969 4543
XbaI T'CTAG_A
Cuts at: 2848 2848
Size: 5512
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5512
XmaI C'CCGG_G
Cuts at: 1870 2837 1870
Size: 967 4545
XmnI GAAnn'nnTTC
Cuts at: 3641 3641
Size: 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3320 276
Size: 53 83 186 936 1786 2468
Fragments arranged by size:
2468 1786 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI
SwaI XcmI