(Linear) (Six-base) MAPSORT of: KIEE6186.seq Check: 3032 from: 1 to: 504 KIEE6186.orf With 182 enzymes: SgfI * December 5, 2014 13:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 129 159 504 Size: 129 30 345 Fragments arranged by size: 345 129 30 AflIII A'CryG_T Cuts at: 0 368 504 Size: 368 136 AleI CACnn'nnGTG Cuts at: 0 56 139 504 Size: 56 83 365 Fragments arranged by size: 365 83 56 AlwNI CAG_nnn'CTG Cuts at: 0 237 504 Size: 237 267 AvaI C'yCGr_G Cuts at: 0 27 504 Size: 27 477 BanI G'GyrC_C Cuts at: 0 154 274 504 Size: 154 120 230 Fragments arranged by size: 230 154 120 BglI GCCn_nnn'nGGC Cuts at: 0 85 352 448 481 504 Size: 85 267 96 33 23 Fragments arranged by size: 267 96 85 33 23 Bme1580I G_kGCm'C Cuts at: 0 182 224 504 Size: 182 42 280 Fragments arranged by size: 280 182 42 BmgBI CAC'GTC Cuts at: 0 177 504 Size: 177 327 BsaAI yAC'GTr Cuts at: 0 219 504 Size: 219 285 BsaBI GATnn'nnATC Cuts at: 0 460 466 504 Size: 460 6 38 Fragments arranged by size: 460 38 6 BsaWI w'CCGG_w Cuts at: 0 190 504 Size: 190 314 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 355 504 Size: 355 149 BsiEI CG_ry'CG Cuts at: 0 244 504 Size: 244 260 Bsp1286I G_dGCh'C Cuts at: 0 182 224 504 Size: 182 42 280 Fragments arranged by size: 280 182 42 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 129 159 222 504 Size: 129 30 63 282 Fragments arranged by size: 282 129 63 30 BstAPI GCAn_nnn'nTGC Cuts at: 0 237 504 Size: 237 267 BstYI r'GATC_y Cuts at: 0 455 461 504 Size: 455 6 43 Fragments arranged by size: 455 43 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 294 504 Size: 294 210 BtsI GCAGTG_nn' Cuts at: 0 320 504 Size: 320 184 EaeI y'GGCC_r Cuts at: 0 36 241 344 504 Size: 36 205 103 160 Fragments arranged by size: 205 160 103 36 EagI C'GGCC_G Cuts at: 0 241 504 Size: 241 263 EarI CTCTTCn'nnn_ Cuts at: 0 333 344 504 Size: 333 11 160 Fragments arranged by size: 333 160 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 10 37 430 504 Size: 10 27 393 74 Fragments arranged by size: 393 74 27 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 490 504 Size: 490 14 EcoO109I rG'GnC_Cy Cuts at: 0 234 483 495 504 Size: 234 249 12 9 Fragments arranged by size: 249 234 12 9 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 414 504 Size: 414 90 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 353 504 Size: 353 151 MslI CAynn'nnrTG Cuts at: 0 56 139 313 504 Size: 56 83 174 191 Fragments arranged by size: 191 174 83 56 MspA1I CmG'CkG Cuts at: 0 302 433 504 Size: 302 131 71 Fragments arranged by size: 302 131 71 NspI r_CATG'y Cuts at: 0 265 372 504 Size: 265 107 132 Fragments arranged by size: 265 132 107 PciI A'CATG_T Cuts at: 0 368 504 Size: 368 136 PmlI CAC'GTG Cuts at: 0 219 504 Size: 219 285 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 288 310 320 342 504 Size: 288 22 10 22 162 Fragments arranged by size: 288 162 22 22 10 PpuMI rG'GwC_Cy Cuts at: 0 234 504 Size: 234 270 PstI C_TGCA'G Cuts at: 0 233 504 Size: 233 271 PvuII CAG'CTG Cuts at: 0 433 504 Size: 433 71 SfcI C'TryA_G Cuts at: 0 229 504 Size: 229 275 SfiI GGCCn_nnn'nGGCC Cuts at: 0 352 481 504 Size: 352 129 23 Fragments arranged by size: 352 129 23 SmaI CCC'GGG Cuts at: 0 29 504 Size: 29 475 SphI G_CATG'C Cuts at: 0 265 504 Size: 265 239 StyI C'CwwG_G Cuts at: 0 389 504 Size: 389 115 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 396 483 504 Size: 396 87 21 Fragments arranged by size: 396 87 21 XmaI C'CCGG_G Cuts at: 0 27 504 Size: 27 477 Enzymes that do cut: AarI AflIII AleI AlwNI AvaI BanI BglI Bme1580I BmgBI BsaAI BsaBI BsaWI BsgI BsiEI Bsp1286I BspMI BstAPI BstYI BtgZI BtsI EaeI EagI EarI EciI EcoNI EcoO109I FalI MmeI MslI MspA1I NspI PciI PmlI PpiI PpuMI PstI PvuII SfcI SfiI SmaI SphI StyI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EcoICRI Eco57MI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI