################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbsI BcgI BciVI BclI BfaI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseRI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrDI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI DpnII DraI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HinfI HpaI KpnI MaeI MamI MboI MfeI MflI MluNI MscI MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI SacI SalI Sau3AI ScaI SexAI SfiI SfuI SnaBI SpeI SphI SspI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 19 TCCGGA T^CCGGA AflII 910 CTTAAG C^TTAAG AspHI 529 GTGCTC G[AT]GC[AT]^C BbrPI 384 CACGTG CAC^GTG BbvCI 489 CCTCAGC CCTCAGC(-5/-2) BfrI 910 CTTAAG C^TTAAG BpmI 598 CTGGAG CTGGAG(16/14) BsaWI 19 TCCGGA [AT]^CCGG[AT] BseAI 19 TCCGGA T^CCGGA BsiEI 797 CGGCCG CG[GA][CT]^CG BsiHKAI 529 GTGCTC G[AT]GC[AT]^C Bsp1407I 617 TGTACA T^GTACA BspEI 19 TCCGGA T^CCGGA BsrBI 136 CCGCTC CCGCTC(-3/-3) BsrGI 617 TGTACA T^GTACA BsrI 97 CCAGT ACTGG(1/-1) BssSI 130 CTCGTG CACGAG(-5/-1) BstXI 404 CCATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 220 CGGACCG CG^G[AT]CCG DdeI 490 CTCAG C^T[ACGT]AG DraIII 4 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 91 GACGGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 797 CGGCCG C^GGCCG EclXI 797 CGGCCG C^GGCCG Eco52I 797 CGGCCG C^GGCCG EcoNI 1170 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 776 CCAAGG C^C[AT][AT]GG HincII 352 GTCAAC GT[CT]^[GA]AC HindII 352 GTCAAC GT[CT]^[GA]AC HphI 550 TCACC GGTGA(8/7) MluI 29 ACGCGT A^CGCGT MroI 19 TCCGGA T^CCGGA MseI 911 TTAA T^TAA NaeI 1046 GCCGGC GCC^GGC NgoMIV 1046 GCCGGC G^CCGGC NspI 929 GCATGT [GA]CATG^[CT] PmaCI 384 CACGTG CAC^GTG PmlI 384 CACGTG CAC^GTG PpuMI 1099 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 838 CAGCTG CAG^CTG RsrII 220 CGGACCG CG^G[AT]CCG SbfI 732 CCTGCAGG CCTGCA^GG SgrAI 524 CGCCGGTG C[GA]^CCGG[CT]G SmlI 910 CTTAAG C^T[CT][GA]AG Sse8387I 732 CCTGCAGG CCTGCA^GG SspBI 617 TGTACA T^GTACA StyI 776 CCAAGG C^C[AT][AT]GG Tru9I 911 TTAA T^TAA XcmI 587 CCAGCCGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 29 ACGCGT A^C[GA][CT]GT 385 ACGTGT ApaI 828 GGGCCC GGGCC^C 864 GGGCCC AvaI 66 CCCGGG C^[CT]CG[GA]G 553 CCCGGG BbeI 76 GGCGCC GGCGC^C 763 GGCGCC BsaAI 384 CACGTG [CT]AC^GT[GA] 424 TACGTG BsaI 369 GGTCTC GGTCTC(1/5) 780 GGTCTC BsmAI 370 GTCTC GTCTC(1/5) 781 GTCTC BsoBI 66 CCCGGG C^[CT]CG[GA]G 553 CCCGGG BstF5I 360 CATCC GGATG(2/0) 834 CATCC EarI 139 CTCTTC CTCTTC(1/4) 811 CTCTTC FokI 360 CATCC GGATG(9/13) 834 CATCC KasI 76 GGCGCC G^GCGCC 763 GGCGCC Ksp632I 139 CTCTTC CTCTTC(1/4) 811 CTCTTC KspI 109 CCGCGG CCGC^GG 869 CCGCGG MslI 258 CACGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 405 CATGAGTGTG NarI 76 GGCGCC GG^CGCC 763 GGCGCC SacII 109 CCGCGG CCGC^GG 869 CCGCGG SapI 138 GCTCTTC GCTCTTC(1/4) 810 GCTCTTC SfoI 76 GGCGCC GGC^GCC 763 GGCGCC SmaI 66 CCCGGG CCC^GGG 553 CCCGGG StuI 578 AGGCCT AGG^CCT 995 AGGCCT XmaCI 66 CCCGGG C^CCGGG 553 CCCGGG XmaI 66 CCCGGG C^CCGGG 553 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AvaII BanI BanII BbvI BcnI BglI BmyI BsaHI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MaeII MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI RsaI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI