(Circular) (Six-base) MAPSORT of: pFN21AE9550.seq Check: 9498 from: 1 to: 5899 pFN21AE9550 5899 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2672 2737 2672 Size: 65 5834 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3242 1937 Size: 1305 4594 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3962 5346 5614 1632 Size: 2330 1384 268 1917 Fragments arranged by size: 2330 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2915 820 Size: 197 1898 3804 Fragments arranged by size: 3804 1898 197 AflIII A'CryG_T Cuts at: 1203 2034 2390 4798 5775 1203 Size: 831 356 2408 977 1327 Fragments arranged by size: 2408 1327 977 831 356 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 2267 1166 Size: 1101 4798 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 2502 2752 2866 3642 5214 2502 Size: 250 114 776 1572 3187 Fragments arranged by size: 3187 1572 776 250 114 ApaI G_GGCC'C Cuts at: 2837 2873 2837 Size: 36 5863 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 3208 3434 3586 5695 1493 Size: 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 2071 2558 3224 1870 Size: 73 128 487 666 4545 Fragments arranged by size: 4545 666 487 128 73 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 2081 2768 2886 3220 Size: 325 217 627 260 34 687 118 334 Cuts at: 3220 4675 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 687 627 334 325 260 217 118 34 BanII G_rGCy'C Cuts at: 1391 2837 2873 2882 2929 2987 3218 1391 Size: 1446 36 9 47 58 231 4072 Fragments arranged by size: 4072 1446 231 58 47 36 9 BbeI G_GCGC'C Cuts at: 2085 2772 2085 Size: 687 5212 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4928 BbvCI CC'TCA_GC Cuts at: 2495 2495 Size: 5899 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4090 4124 1903 Size: 34 50 34 2069 34 3678 Fragments arranged by size: 3678 2069 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2255 2697 2777 Size: 107 122 71 631 605 583 442 80 Cuts at: 2777 2877 4510 136 Size: 100 1633 1525 Fragments arranged by size: 1633 1525 631 605 583 442 122 107 100 80 71 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 3316 3316 Size: 5899 Bme1580I G_kGCm'C Cuts at: 1790 2050 2837 2873 3960 5116 1790 Size: 260 787 36 1087 1156 2573 Fragments arranged by size: 2573 1156 1087 787 260 36 BmgBI CAC'GTC Cuts at: 1318 2269 1318 Size: 951 4948 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2096 3630 4588 Size: 416 53 83 399 24 679 1534 958 Cuts at: 4588 442 Size: 1753 Fragments arranged by size: 1753 1534 958 679 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 4559 1239 Size: 1385 1935 2579 Fragments arranged by size: 2579 1935 1385 Bpu10I CC'TnA_GC Cuts at: 2495 2495 Size: 5899 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4024 4889 5187 4024 Size: 865 298 4736 Fragments arranged by size: 4736 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2380 2791 4562 882 Size: 617 881 411 1771 2219 Fragments arranged by size: 2219 1771 881 617 411 BsaAI yAC'GTr Cuts at: 493 1204 2391 2431 3690 493 Size: 711 1187 40 1259 2702 Fragments arranged by size: 2702 1259 1187 711 40 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 2127 2769 3706 Size: 53 83 186 936 549 45 642 937 Cuts at: 3706 4088 4723 275 Size: 382 635 1451 Fragments arranged by size: 1451 937 936 642 635 549 382 186 83 53 45 BsaWI w'CCGG_w Cuts at: 2024 4332 4730 5004 5151 5562 2024 Size: 2308 398 274 147 411 2361 Fragments arranged by size: 2361 2308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2933 2963 1178 Size: 30 165 30 1530 30 4114 Fragments arranged by size: 4114 1530 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5899 BseYI C'CCAG_C Cuts at: 2167 2325 2332 2591 2829 2866 5102 5598 2167 Size: 158 7 259 238 37 2236 496 2468 Fragments arranged by size: 2468 2236 496 259 238 158 37 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 2441 2528 3077 1163 Size: 573 666 39 87 549 3985 Fragments arranged by size: 3985 666 573 549 87 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2805 3349 4110 5138 1540 Size: 275 186 804 544 761 1028 2301 Fragments arranged by size: 2301 1028 804 761 544 275 186 BsiHKAI G_wGCw'C Cuts at: 2538 3960 4045 5116 2538 Size: 1422 85 1071 3321 Fragments arranged by size: 3321 1422 1071 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2538 2837 2873 2882 2929 2987 Size: 399 260 488 299 36 9 47 58 Cuts at: 2987 3218 3960 4045 5116 1391 Size: 231 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 488 399 299 260 231 85 58 47 36 9 BspEI T'CCGG_A Cuts at: 2024 5562 2024 Size: 3538 2361 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2362 2637 2652 2672 2737 3254 844 Size: 1518 275 15 20 65 517 3489 Fragments arranged by size: 3489 1518 517 275 65 20 15 BsrBI CCG'CTC Cuts at: 1394 1823 2143 3786 1394 Size: 429 320 1643 3507 Fragments arranged by size: 3507 1643 429 320 BsrDI GCAATG_nn' Cuts at: 65 1248 4394 4568 65 Size: 1183 3146 174 1396 Fragments arranged by size: 3146 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2050 2530 3051 4543 4730 1700 Size: 110 240 480 521 1492 187 2869 Fragments arranged by size: 2869 1492 521 480 240 187 110 BsrGI T'GTAC_A Cuts at: 96 2622 96 Size: 2526 3373 BssHII G'CGCG_C Cuts at: 1925 2449 2566 2608 2761 3024 3158 1925 Size: 524 117 42 153 263 134 4666 Fragments arranged by size: 4666 524 263 153 134 117 42 BssSI C'ACGA_G Cuts at: 2135 3959 4971 2135 Size: 1824 1012 3063 Fragments arranged by size: 3063 1824 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5899 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstXI CCAn_nnnn'nTGG Cuts at: 2416 2416 Size: 5899 BstYI r'GATC_y Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894 Size: 1257 367 385 17 1452 86 12 346 Cuts at: 5894 1972 Size: 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2013 2114 2121 2262 2805 2874 513 Size: 552 948 101 7 141 543 69 3538 Fragments arranged by size: 3538 948 552 543 141 101 69 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2230 487 Size: 683 56 1004 4156 Fragments arranged by size: 4156 1004 683 56 BtsI GCAGTG_nn' Cuts at: 2584 2929 3151 3415 4209 4229 2584 Size: 345 222 264 794 20 4254 Fragments arranged by size: 4254 794 345 264 222 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DraIII CAC_nnn'GTG Cuts at: 2014 2014 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2102 4906 809 Size: 507 786 2804 1802 Fragments arranged by size: 2804 1802 786 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2259 2679 Size: 54 1425 50 275 18 222 207 420 Cuts at: 2679 2802 3153 3346 4235 5864 8 Size: 123 351 193 889 1629 43 Fragments arranged by size: 1629 1425 889 420 351 275 222 207 193 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2802 3346 1537 Size: 275 990 544 4090 Fragments arranged by size: 4090 990 544 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2150 2822 3829 1353 Size: 797 672 1007 3423 Fragments arranged by size: 3423 1007 797 672 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2996 4486 4860 5006 387 Size: 1064 138 1407 1490 374 146 1280 Fragments arranged by size: 1490 1407 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2624 3962 4559 5346 5614 1239 Size: 393 992 1338 597 787 268 1524 Fragments arranged by size: 1524 1338 992 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3179 3179 Size: 5899 EcoO109I rG'GnC_Cy Cuts at: 2833 2834 2870 3105 2833 Size: 1 36 235 5627 Fragments arranged by size: 5627 235 36 1 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 2085 2159 2483 2639 2736 2772 2867 5046 Size: 407 74 324 156 97 36 95 2179 Cuts at: 5046 1678 Size: 2531 Fragments arranged by size: 2531 2179 407 324 156 97 95 74 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2911 2943 4540 4572 4614 Size: 32 135 32 1339 32 1597 32 42 Cuts at: 4614 4646 1373 Size: 32 2626 Fragments arranged by size: 2626 1597 1339 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2359 3243 3489 669 Size: 988 281 421 884 246 3079 Fragments arranged by size: 3079 988 884 421 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KasI G'GCGC_C Cuts at: 2081 2768 2081 Size: 687 5212 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 2034 5775 2034 Size: 3741 2158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3019 5013 5197 1305 Size: 1714 1994 184 2007 Fragments arranged by size: 2007 1994 1714 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4077 Fragments arranged by size: 4077 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2414 3857 4216 4375 518 Size: 648 1101 147 1443 359 159 2042 Fragments arranged by size: 2042 1443 1101 648 359 159 147 MspA1I CmG'CkG Cuts at: 1606 2116 2317 2424 2653 2845 2876 2965 3315 Size: 510 201 107 229 192 31 89 350 Cuts at: 3315 3992 5140 5385 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 510 350 245 229 201 192 107 89 31 NaeI GCC'GGC Cuts at: 3053 3053 Size: 5899 NarI GG'CG_CC Cuts at: 2082 2769 2082 Size: 687 5212 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5347 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NgoMIV G'CCGG_C Cuts at: 3051 3051 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 2938 3257 4802 1132 Size: 97 1709 319 1545 2229 Fragments arranged by size: 2229 1709 1545 319 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5899 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5899 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PmlI CAC'GTG Cuts at: 2391 2391 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3105 3105 Size: 5899 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspOMI G'GGCC_C Cuts at: 2833 2869 2833 Size: 36 5863 PstI C_TGCA'G Cuts at: 830 2579 2742 2862 3251 830 Size: 1749 163 120 389 3478 Fragments arranged by size: 3478 1749 389 163 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 PvuII CAG'CTG Cuts at: 2845 2845 Size: 5899 RsrII CG'GwC_CG Cuts at: 2226 2226 Size: 5899 SacII CC_GC'GG Cuts at: 2117 2877 2117 Size: 760 5139 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SanDI GG'GwC_CC Cuts at: 3105 3105 Size: 5899 SapI GCTCTTCn'nnn_ Cuts at: 2150 2822 2150 Size: 672 5227 SbfI CC_TGCA'GG Cuts at: 2742 3251 2742 Size: 509 5390 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SfcI C'TryA_G Cuts at: 826 1045 2575 2738 2858 2941 3247 4382 5063 Size: 219 1530 163 120 83 306 1135 681 Cuts at: 5063 5254 826 Size: 191 1471 Fragments arranged by size: 1530 1471 1135 681 306 219 191 163 120 83 SfoI GGC'GCC Cuts at: 2083 2770 2083 Size: 687 5212 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 2530 1700 Size: 830 5069 SmaI CCC'GGG Cuts at: 1872 2073 2560 3226 1872 Size: 201 487 666 4545 Fragments arranged by size: 4545 666 487 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 2915 4003 4904 5166 820 Size: 197 926 972 1088 901 262 1553 Fragments arranged by size: 1553 1088 972 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SrfI GCCC'GGGC Cuts at: 2073 2073 Size: 5899 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 2585 3002 1517 Size: 1068 417 4414 Fragments arranged by size: 4414 1068 417 StyI C'CwwG_G Cuts at: 513 1065 2781 3338 513 Size: 552 1716 557 3074 Fragments arranged by size: 3074 1716 557 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2317 2904 3926 3943 4096 4281 4726 Size: 1255 737 587 1022 17 153 185 445 Cuts at: 4726 325 Size: 1498 Fragments arranged by size: 1498 1255 1022 737 587 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2622 4145 5857 Size: 274 80 33 51 1443 645 1523 1712 Cuts at: 5857 96 Size: 138 Fragments arranged by size: 1712 1523 1443 645 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2921 2953 5859 5891 2921 Size: 32 2906 32 2929 Fragments arranged by size: 2929 2906 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2599 2011 Size: 588 5311 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 2071 2558 3224 1870 Size: 201 487 666 4545 Fragments arranged by size: 4545 666 487 201 XmnI GAAnn'nnTTC Cuts at: 4028 4028 Size: 5899 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI SacI SexAI SfiI SwaI