Restriction map for
pFN21AE1750
(Circular) (Six-base) MAPSORT of: pFN21AE1750.seq Check: 8160 from: 1 to: 5464
pFN21AE1750 5464 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2546 2546
Size: 5464
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3527 4911 5179 1632
Size: 1895 1384 268 1917
Fragments arranged by size:
1917 1895 1384 268
AfeI AGC'GCT
Cuts at: 2579 2579
Size: 5464
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 2723 1166
Size: 1557 3907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AlwNI CAG_nnn'CTG
Cuts at: 3207 4779 3207
Size: 1572 3892
ApaI G_GGCC'C
Cuts at: 2387 2636 2387
Size: 249 5215
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2657 2674 2708 2789 1870
Size: 73 714 17 34 81 4545
Fragments arranged by size:
4545 714 81 73 34 17
AvrII C'CTAG_G
Cuts at: 2041 2041
Size: 5464
BamHI G'GATC_C
Cuts at: 2079 2794 3161 2079
Size: 715 367 4382
Fragments arranged by size:
4382 715 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 2431 2564 2785 4240
Size: 325 217 627 275 369 133 221 1455
Cuts at: 4240 618
Size: 1842
Fragments arranged by size:
1842 1455 627 369 325 275 221 217
133
BanII G_rGCy'C
Cuts at: 1391 2274 2387 2636 2681 2783 1391
Size: 883 113 249 45 102 4072
Fragments arranged by size:
4072 883 249 113 102 45
BbeI G_GCGC'C
Cuts at: 2066 2568 2066
Size: 502 4962
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3655 3689 1903
Size: 34 50 34 1634 34 3678
Fragments arranged by size:
3678 1634 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 4075 136
Size: 107 122 71 631 605 388 2015 1525
Fragments arranged by size:
2015 1525 631 605 388 122 107 71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2189 2881 2189
Size: 692 4772
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2387 2636 3525 4681 1790
Size: 520 77 249 889 1156 2573
Fragments arranged by size:
2573 1156 889 520 249 77
BmgBI CAC'GTC
Cuts at: 1318 2293 2428 2452 1318
Size: 975 135 24 4330
Fragments arranged by size:
4330 975 135 24
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2390 3195 4153
Size: 416 53 83 399 24 973 805 958
Cuts at: 4153 442
Size: 1753
Fragments arranged by size:
1753 973 958 805 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 4124 1239
Size: 989 1896 2579
Fragments arranged by size:
2579 1896 989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3589 4454 4752 3589
Size: 865 298 4301
Fragments arranged by size:
4301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4127 882
Size: 617 2628 2219
Fragments arranged by size:
2628 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2509 3255 493
Size: 711 1305 746 2702
Fragments arranged by size:
2702 1305 746 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 2126 2163 2565
Size: 53 83 186 936 530 63 37 402
Cuts at: 2565 3271 3653 4288 275
Size: 706 382 635 1451
Fragments arranged by size:
1451 936 706 635 530 402 382 186
83 63 53 37
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5239
Fragments arranged by size:
5239 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5464
BseYI C'CCAG_C
Cuts at: 2013 2460 4667 5163 2013
Size: 447 2207 496 2314
Fragments arranged by size:
2314 2207 496 447
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2252 1163
Size: 573 417 99 4375
Fragments arranged by size:
4375 573 417 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2220 2914 3675 4703 1540
Size: 275 186 51 168 694 761 1028 2301
Fragments arranged by size:
2301 1028 761 694 275 186 168 51
BsiHKAI G_wGCw'C
Cuts at: 2298 2514 2658 3525 3610 4681 2298
Size: 216 144 867 85 1071 3081
Fragments arranged by size:
3081 1071 867 216 144 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 2298 2310 2387 2514 2636 2658
Size: 399 484 24 12 77 127 122 22
Cuts at: 2658 2681 2783 3525 3610 4681 1391
Size: 23 102 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 399 127 122 102
85 77 24 23 22 12
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2224 2546 2819 844
Size: 1380 322 273 3489
Fragments arranged by size:
3489 1380 322 273
BsrBI CCG'CTC
Cuts at: 1394 1823 2048 3351 1394
Size: 429 225 1303 3507
Fragments arranged by size:
3507 1303 429 225
BsrDI GCAATG_nn'
Cuts at: 65 1248 3959 4133 65
Size: 1183 2711 174 1396
Fragments arranged by size:
2711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2456 2544 4108 4295 1700
Size: 110 646 88 1564 187 2869
Fragments arranged by size:
2869 1564 646 187 110 88
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 2145 2247 1925
Size: 220 102 5142
Fragments arranged by size:
5142 220 102
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2185 2509 794
Size: 1391 324 3749
Fragments arranged by size:
3749 1391 324
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 2668 1184
Size: 1484 3980
BstYI r'GATC_y
Cuts at: 1972 2079 2712 2794 3161 3546 3563 5015 5101
Size: 107 633 82 367 385 17 1452 86
Cuts at: 5101 5113 5459 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 633 385 367 346 107 86
82 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2071 513
Size: 552 1006 3906
Fragments arranged by size:
3906 1006 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2186 2200 2310 2980 3774 3794 2186
Size: 14 110 670 794 20 3856
Fragments arranged by size:
3856 794 670 110 20 14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2217 2454
Size: 54 1425 50 275 18 219 168 237
Cuts at: 2454 2760 2911 3800 5429 8
Size: 306 151 889 1629 43
Fragments arranged by size:
1629 1425 889 306 275 237 219 168
151 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2049 2217 2911 1537
Size: 275 237 168 694 4090
Fragments arranged by size:
4090 694 275 237 168
EarI CTCTTCn'nnn_
Cuts at: 1353 3394 1353
Size: 2041 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2189 4051 4425 4571 387
Size: 1064 138 600 1862 374 146 1280
Fragments arranged by size:
1862 1280 1064 600 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 3527 4124 4911 5179 1239
Size: 393 596 1299 597 787 268 1524
Fragments arranged by size:
1524 1299 787 597 596 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2384 2632 2384
Size: 248 5216
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 2023 2066 2284 2568 2581 4611 1678
Size: 345 43 218 284 13 2030 2531
Fragments arranged by size:
2531 2030 345 284 218 43 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4105 4137 4179 4211 1373
Size: 32 135 32 2533 32 42 32 2626
Fragments arranged by size:
2626 2533 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 2808 3054 669
Size: 988 281 535 335 246 3079
Fragments arranged by size:
3079 988 535 335 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KasI G'GCGC_C
Cuts at: 2062 2564 2062
Size: 502 4962
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2057 2346 4578 4762 1305
Size: 752 289 2232 184 2007
Fragments arranged by size:
2232 2007 752 289 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3642
Fragments arranged by size:
3642 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2723 3422 3781 3940 518
Size: 648 1557 699 359 159 2042
Fragments arranged by size:
2042 1557 699 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2137 2362 2613 2638 2880 3557 4705
Size: 407 124 225 251 25 242 677 1148
Cuts at: 4705 4950 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 407 251 245 242 225
124 25
NaeI GCC'GGC
Cuts at: 2458 2458
Size: 5464
NarI GG'CG_CC
Cuts at: 2063 2565 2063
Size: 502 4962
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4912
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NgoMIV G'CCGG_C
Cuts at: 2456 2456
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2049 2911 2049
Size: 862 4602
NspI r_CATG'y
Cuts at: 1132 1229 2822 4367 1132
Size: 97 1593 1545 2229
Fragments arranged by size:
2229 1593 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5464
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PmlI CAC'GTG
Cuts at: 2509 2509
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PspOMI G'GGCC_C
Cuts at: 2383 2632 2383
Size: 249 5215
PspXI vC'TCGA_Gb
Cuts at: 2657 2657
Size: 5464
PstI C_TGCA'G
Cuts at: 830 2505 2816 830
Size: 1675 311 3478
Fragments arranged by size:
3478 1675 311
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
PvuII CAG'CTG
Cuts at: 2013 2137 2362 2613 2013
Size: 124 225 251 4864
Fragments arranged by size:
4864 251 225 124
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2357 2501 2812 3947 4628 4819 826
Size: 219 1312 144 311 1135 681 191 1471
Fragments arranged by size:
1471 1312 1135 681 311 219 191 144
SfoI GGC'GCC
Cuts at: 2064 2566 2064
Size: 502 4962
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2710 2791 1872
Size: 838 81 4545
Fragments arranged by size:
4545 838 81
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 3568 4469 4731 820
Size: 197 926 714 911 901 262 1553
Fragments arranged by size:
1553 926 911 901 714 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5464
StyI C'CwwG_G
Cuts at: 513 1065 2041 2903 513
Size: 552 976 862 3074
Fragments arranged by size:
3074 976 862 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 3491 3508 3661 3846 4291 325
Size: 1255 593 1318 17 153 185 445 1498
Fragments arranged by size:
1498 1318 1255 593 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3710 5422 96
Size: 274 80 33 51 1443 1733 1712 138
Fragments arranged by size:
1733 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5424 5456 5424
Size: 32 5432
Tth111I GACn'n_nGTC
Cuts at: 1316 2753 1316
Size: 1437 4027
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XhoI C'TCGA_G
Cuts at: 1943 2657 1943
Size: 714 4750
XmaI C'CCGG_G
Cuts at: 1870 2708 2789 1870
Size: 838 81 4545
Fragments arranged by size:
4545 838 81
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PpuMI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI