(Linear) (Six-base) MAPSORT of: KIEE1750.seq Check: 4476 from: 1 to: 759 KIEE1750.orf With 182 enzymes: SgfI * July 15, 2010 18:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 541 759 Size: 541 218 AfeI AGC'GCT Cuts at: 0 574 759 Size: 574 185 AleI CACnn'nnGTG Cuts at: 0 718 759 Size: 718 41 ApaI G_GGCC'C Cuts at: 0 382 631 759 Size: 382 249 128 Fragments arranged by size: 382 249 128 AvaI C'yCGr_G Cuts at: 0 652 669 703 759 Size: 652 17 34 56 Fragments arranged by size: 652 56 34 17 AvrII C'CTAG_G Cuts at: 0 36 759 Size: 36 723 BamHI G'GATC_C Cuts at: 0 74 759 Size: 74 685 BanI G'GyrC_C Cuts at: 0 57 426 559 759 Size: 57 369 133 200 Fragments arranged by size: 369 200 133 57 BanII G_rGCy'C Cuts at: 0 269 382 631 676 759 Size: 269 113 249 45 83 Fragments arranged by size: 269 249 113 83 45 BbeI G_GCGC'C Cuts at: 0 61 563 759 Size: 61 502 196 Fragments arranged by size: 502 196 61 BglI GCCn_nnn'nGGC Cuts at: 0 55 759 Size: 55 704 BlpI GC'TnA_GC Cuts at: 0 184 759 Size: 184 575 Bme1580I G_kGCm'C Cuts at: 0 305 382 631 759 Size: 305 77 249 128 Fragments arranged by size: 305 249 128 77 BmgBI CAC'GTC Cuts at: 0 288 423 447 759 Size: 288 135 24 312 Fragments arranged by size: 312 288 135 24 BmrI ACTGGGnnnn_n' Cuts at: 0 385 759 Size: 385 374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 759 Size: 223 536 BsaAI yAC'GTr Cuts at: 0 504 759 Size: 504 255 BsaHI Gr'CG_yC Cuts at: 0 58 121 158 560 759 Size: 58 63 37 402 199 Fragments arranged by size: 402 199 63 58 37 BseYI C'CCAG_C Cuts at: 0 8 455 759 Size: 8 447 304 Fragments arranged by size: 447 304 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 247 759 Size: 148 99 512 Fragments arranged by size: 512 148 99 BsiEI CG_ry'CG Cuts at: 0 47 215 759 Size: 47 168 544 Fragments arranged by size: 544 168 47 BsiHKAI G_wGCw'C Cuts at: 0 293 509 653 759 Size: 293 216 144 106 Fragments arranged by size: 293 216 144 106 Bsp1286I G_dGCh'C Cuts at: 0 269 293 305 382 509 631 653 676 Size: 269 24 12 77 127 122 22 23 Cuts at: 676 759 Size: 83 Fragments arranged by size: 269 127 122 83 77 24 23 22 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 219 541 759 Size: 219 322 218 Fragments arranged by size: 322 219 218 BsrBI CCG'CTC Cuts at: 0 43 759 Size: 43 716 BsrFI r'CCGG_y Cuts at: 0 451 539 759 Size: 451 88 220 Fragments arranged by size: 451 220 88 BssHII G'CGCG_C Cuts at: 0 140 242 759 Size: 140 102 517 Fragments arranged by size: 517 140 102 BstAPI GCAn_nnn'nTGC Cuts at: 0 180 504 759 Size: 180 324 255 Fragments arranged by size: 324 255 180 BstEII G'GTnAC_C Cuts at: 0 663 759 Size: 663 96 BstYI r'GATC_y Cuts at: 0 74 707 759 Size: 74 633 52 Fragments arranged by size: 633 74 52 BtgI C'CryG_G Cuts at: 0 66 759 Size: 66 693 BtsI GCAGTG_nn' Cuts at: 0 181 195 305 759 Size: 181 14 110 454 Fragments arranged by size: 454 181 110 14 EaeI y'GGCC_r Cuts at: 0 44 212 449 759 Size: 44 168 237 310 Fragments arranged by size: 310 237 168 44 EagI C'GGCC_G Cuts at: 0 44 212 759 Size: 44 168 547 Fragments arranged by size: 547 168 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 184 759 Size: 184 575 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 759 Size: 223 536 EcoO109I rG'GnC_Cy Cuts at: 0 379 627 759 Size: 379 248 132 Fragments arranged by size: 379 248 132 HaeII r_GCGC'y Cuts at: 0 18 61 279 563 576 759 Size: 18 43 218 284 13 183 Fragments arranged by size: 284 218 183 43 18 13 HincII GTy'rAC Cuts at: 0 468 759 Size: 468 291 KasI G'GCGC_C Cuts at: 0 57 559 759 Size: 57 502 200 Fragments arranged by size: 502 200 57 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 52 341 759 Size: 52 289 418 Fragments arranged by size: 418 289 52 MslI CAynn'nnrTG Cuts at: 0 718 759 Size: 718 41 MspA1I CmG'CkG Cuts at: 0 8 132 357 608 633 759 Size: 8 124 225 251 25 126 Fragments arranged by size: 251 225 126 124 25 8 NaeI GCC'GGC Cuts at: 0 453 759 Size: 453 306 NarI GG'CG_CC Cuts at: 0 58 560 759 Size: 58 502 199 Fragments arranged by size: 502 199 58 NgoMIV G'CCGG_C Cuts at: 0 451 759 Size: 451 308 NotI GC'GGCC_GC Cuts at: 0 44 759 Size: 44 715 PmlI CAC'GTG Cuts at: 0 504 759 Size: 504 255 PspOMI G'GGCC_C Cuts at: 0 378 627 759 Size: 378 249 132 Fragments arranged by size: 378 249 132 PspXI vC'TCGA_Gb Cuts at: 0 652 759 Size: 652 107 PstI C_TGCA'G Cuts at: 0 500 759 Size: 500 259 PvuII CAG'CTG Cuts at: 0 8 132 357 608 759 Size: 8 124 225 251 151 Fragments arranged by size: 251 225 151 124 8 SfcI C'TryA_G Cuts at: 0 352 496 759 Size: 352 144 263 Fragments arranged by size: 352 263 144 SfoI GGC'GCC Cuts at: 0 59 561 759 Size: 59 502 198 Fragments arranged by size: 502 198 59 SmaI CCC'GGG Cuts at: 0 705 759 Size: 705 54 SmlI C'TyrA_G Cuts at: 0 652 759 Size: 652 107 StyI C'CwwG_G Cuts at: 0 36 759 Size: 36 723 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 168 759 Size: 168 591 Tth111I GACn'n_nGTC Cuts at: 0 748 759 Size: 748 11 XhoI C'TCGA_G Cuts at: 0 652 759 Size: 652 107 XmaI C'CCGG_G Cuts at: 0 703 759 Size: 703 56 Enzymes that do cut: AarI AfeI AleI ApaI AvaI AvrII BamHI BanI BanII BbeI BglI BlpI Bme1580I BmgBI BmrI BpmI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII BstYI BtgI BtsI EaeI EagI EciI Eco57MI EcoO109I HaeII HincII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NotI PmlI PspOMI PspXI PstI PvuII SfcI SfoI SmaI SmlI StyI TaqII Tth111I XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI BaeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI XcmI XmnI ZraI